Shallot is an important crop as a condiment as well as a source of income for smallholder farmers in Ethiopia. However, the yield of the crop is constrained by a number of factors among which inappropriate plant spacing and lack of improved varieties are the bottlenecks of its productivity in the study area. Therefore, a field experiment was conducted at Densa, Eastern Amhara, and Ethiopia to evaluate the growth and yield response of shallot varieties to intra-row spacing. The treatments consisted of four intra-row spacings (5, 10, 15 and 20 cm) and three shallot varieties (Dz-sht-157-1B, Dz-sht-91-2B, and Yheras). These were laid out in randomized complete block design replicated three times. Data on growth and yield parameters were recorded and subjected to analysis of variance (ANOVA). Results indicated that varying intra-row spacings from 5 to 20 cm increased significantly (P <0.01) all yield characters assessed in all the traits except cured bulb yield (t ha-1) which decreased as a result of increasing intra-row spacing from 5 to 20 cm. Highest total bulb yield (26.20 t ha-1) and marketable bulb yields (25.24 t ha-1) were recorded at the closest intra-row spacing (5 cm). Dz-sht-157-1B variety was superior in terms of leaf number per plant (35.82), average bulb weight (69.08 g), marketable yield (24.46 t ha-1) and total bulb yield (25.17 t ha-1). Hence, based on the result, Dz-sht-91-2B grown at 10 cm intra-row spacing should be adopted by farmers in study area. However, further investigations on multiplications and over years should be made to come up with complete recommendations. Contribution/Originality: This study contributes to the existing literature by conducting a field experiment at Densa, Eastern Amhara, and Ethiopia to evaluate the growth and yield response of shallot varieties to intra-row spacing. 1. INTRODUCTION Shallot (Allium cepa var. aggregatum), belongs to the family Alliaceae, is an onion like plant that is originated from Western Asia [1]. The crop is grown in more than 170 countries in the world that China and India are the world's largest producers [2]. It is one of the most widely cultivated bulb crops with wide range of climatic and soil adaptation in Ethiopia [3]. The crop is produced traditionally by small farmers in Hararge, Shoa, Arsi, Gojjam, etc. as cash crops and income generating spices for flavoring local dishes. Shallot is also preferred for its shorter growth cycle, better tolerance to disease and drought stresses and longer storage life than the common onion and for its distinct flavor that persists after cooking [4].