Effect of Intracellular ATP Levels on the Light-Induced H&lt;sup&gt;+&lt;/sup&gt; Efflux from Intact Cells of &lt;italic&gt;Cyanidium caldarium&lt;/italic&gt;&lt;xref ref-type="fn" rid="fn1"&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/xref&gt;

Enami, Isao; Kura-Hotta, Mariko

doi:10.1093/oxfordjournals.pcp.a076817

Cited by 4 publications

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“…In addition, cyanidialean red algae (C. merolae, Cyanidium spp., and Galdieria spp.) inhabit acidic environments, and a previous study of Cyanidium caldarium suggested that ATP is consumed by the plasma membrane ATPase to pump out H+ and prevents acidification of the cytosol (Enami & Kura-Hotta, 1984).…”

Section: Establishment and Property Of Heterotrophic C Merolae Celmentioning

confidence: 99%

Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae

et al. 2019

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The unicellular thermoacidophilic red alga Cyanidioschyzon merolae is an emerging model organism of photosynthetic eukaryotes. Its relatively simple genome (16.5 Mbp) with very low‐genetic redundancy and its cellular structure possessing one chloroplast, mitochondrion, peroxisome, and other organelles have facilitated studies. In addition, this alga is genetically tractable, and the nuclear and chloroplast genomes can be modified by integration of transgenes via homologous recombination. Recent studies have attempted to clarify the structure and function of the photosystems of this alga. However, it is difficult to obtain photosynthesis‐defective mutants for molecular genetic studies because this organism is an obligate autotroph. To overcome this issue in C. merolae , we expressed a plasma membrane sugar transporter, Gs SPT 1, from Galdieria sulphuraria , which is an evolutionary relative of C. merolae and capable of heterotrophic growth. The heterologously expressed GsSPT1 localized at the plasma membrane. GsSPT1 enabled C. merolae to grow mixotrophically and heterotrophically, in which cells grew in the dark with glucose or in the light with a photosynthetic inhibitor 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea ( DCMU ) and glucose. When the GsSPT1 transgene multiplied on the C. merolae chromosome via the URA Cm‐Gs selection marker, which can multiply itself and its flanking transgene, GsSPT1 protein level increased and the heterotrophic and mixotrophic growth of the transformant accelerated. We also found that Gs SPT 1 overexpressing C. merolae efficiently formed colonies on solidified medium under light with glucose and DCMU . Thus, Gs SPT 1 overexpresser will facilitate single colony isolation and analyses of photosynthesis‐deficient mutants produced either by random or site‐directed mutagenesis. In addition, our results yielded evidence supporting that the presence or absence of plasma membrane sugar transporters is a major cause of difference in trophic properties between C. merolae and G. sulphuraria .

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Section: Establishment and Property Of Heterotrophic C Merolae Celmentioning

confidence: 99%

Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae

et al. 2019

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Cloning and sequencing of the gene encoding the plasma membrane H+-ATPase from an acidophilic red alga, Cyanidium caldarium1The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank nucleotide sequence databases with the following accession number D88424.1

Ohta¹,

Shirakawa²,

Uchida³

et al. 1997

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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A cDNA containing an open reading frame encoding the putative plasma membrane H(+)-ATPase in an acidophilic red alga, Cyanidium caldarium, was cloned and sequenced by means of PCR and Southern hybridization based on homologous sequences of P-type ATPases found in other organisms. The cloned cDNA is 3300 bp in length, containing a 2865 bp open reading frame encoding a polypeptide of 955 amino acids which has a predicted molecular mass of 105,371. The deduced amino acid sequence was found to be more homologous to those of P-type H(+)-ATPases from higher plants than that from the green alga Dunaliella bioculata.

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Groß¹

2000

Hydrobiologia

148

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Effect of Intracellular ATP Levels on the Light-Induced H<sup>+</sup> Efflux from Intact Cells of <italic>Cyanidium caldarium</italic><xref ref-type="fn" rid="fn1"><sup>1</sup></xref>

Cited by 4 publications

References 0 publications

Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae

Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae

Cloning and sequencing of the gene encoding the plasma membrane H+-ATPase from an acidophilic red alga, Cyanidium caldarium1The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank nucleotide sequence databases with the following accession number D88424.1

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