Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon

Guarino, Michele Pier Luca; Altomare, Annamaria; Barera, Simone; Locato, Vittoria; Cocca, Silvia; Franchin, Cinzia; Arrigoni, Giorgio; Vannini, Candida; Grossi, Sarah; Campomenosi, Paola; Pasqualetti, Valentina; Bracale, Marcella; Alloni, Rossana; Gara, Laura De; Cicala, Michele

doi:10.1371/journal.pone.0169481

Cited by 19 publications

(14 citation statements)

References 51 publications

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“…Data from a recent study confirm the protective effect of inulin on LPS-induced colonic mucosal oxidative stress and muscle impairment. Using iTRAQ analysis, it was demonstrated that inulin restored the level of some important protective proteins involved in inflammatory processes, and it was able to avoid smooth muscle contraction impairment, preventing the LPS-dependent modification of some proteins involved in intestinal smooth muscle contraction [45]. Some of the most significant effects of inulin-type prebiotics are summarized in Table 1.…”

Section: Fructansmentioning

confidence: 99%

Mechanisms of Action of Prebiotics and Their Effects on Gastro-Intestinal Disorders in Adults

et al. 2020

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In recent years, research has focused on the use of dietary fibers and prebiotics, since many of these polysaccharides can be metabolized by intestinal microbiota, leading to the production of short-chain fatty acids. The metabolites of prebiotic fermentation also show anti-inflammatory and immunomodulatory capabilities, suggesting an interesting role in the treatment of several pathological conditions. Galacto-oligosaccharide and short-and long-chain fructans (Fructo-oligosaccharides and inulin) are the most studied prebiotics, even if other dietary compounds seem to show the same features. There is an increasing interest in dietary strategies to modulate microbiota. The aim of this review is to explore the mechanisms of action of prebiotics and their effects on the principal gastro-intestinal disorders in adults, with a special focus on Galacto-oligosaccharides, Fructo-oligosaccharides, lactulose and new emerging substances which currently have evidence of prebiotics effects, such as xilooligosaccharides, soybean oligosaccharides, isomaltooligosaccharides, lactobionic acid, resistant starch and polyphenols.

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Section: Fructansmentioning

confidence: 99%

Mechanisms of Action of Prebiotics and Their Effects on Gastro-Intestinal Disorders in Adults

et al. 2020

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“…On the other hand, incorporating inulin in a diet might exert beneficial systemic effects, despite the lack of desirable changes in the large intestinal environment and microbial activity [ 8 , 9 , 33 , 34 ]. As for the colon, recent findings have demonstrated that inulin alleviated negative effects of oxidative stress induced by lipopolysaccharide [ 11 , 12 ]. In the current study, no challenge was applied, which could increase the production of reactive oxygen species in the colon tissue of piglets above the basal level.…”

Section: Discussionmentioning

confidence: 99%

“…These results are consistent with former findings indicating decreased concentration of thiobarbituric acid-reactive substances, a biomarker of lipid peroxidation, in the liver of piglets fed inulin-supplemented diets [ 9 ]. The results obtained so far have indicated that, in the case of oxidative stress induced in the colon, inulin restored the level of DNA-(apurinic or apyrimidinic site) lyase [ 12 ], which is the DNA base excision repair protein and redox signalling factor maintaining transcription factors in an active reduced state [ 36 ]. This finding suggests that decreased excision activities in piglets resulted from the reduced level of DNA adducts and not from the decreased expression of genes encoding DNA repair enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…This polysaccharide is fermented by intestinal microbiota to short-chain fatty acids [ 3 ] and was thought to act primarily as prebiotic improving host’s health by stimulating the growth of beneficial bacteria in the intestine [ 4 ]. Recently, it has been shown that inulin is an antioxidant exerting desirable effects in the liver of piglets at the level of 1–3% [ 9 , 10 ], in vitro [ 11 ] and ex vivo in human colonic mucosa exposed to lipopolysaccharide [ 12 ]. This activity of inulin may be of crucial importance for piglets during peri-weaning period, which is associated with health problems, such as intestinal bacteria overgrowth and diarrhoea [ 13 ], but also with oxidative stress [ 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

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The effect of dietary level of two inulin types differing in chain length on biogenic amine concentration, oxidant-antioxidant balance and DNA repair in the colon of piglets

et al. 2018

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The effect of dietary level of two types of inulin on amine concentration, redox status and DNA glycosylase activity in the colon of piglets was investigated. Seven groups of piglets were fed diets without inulin addition (control) or with 1%, 2% or 3% inulin with an average degree of polymerisation of 10 (IN10) or 23 (IN23) for 40 days. The 2% and 3% IN10 diets increased tryptamine concentration in the proximal colon, while methylamine concentration in the distal colon was increased by the 1% and 3% IN10 diets. The 1% and 2% IN23 diets increased phenylethylamine and methylamine concentration in the proximal colon, respectively, while 1,7-diaminoheptane content was increased by both diets. Its concentration in the middle and distal colon was increased by the 1% and 2% IN23 diet, respectively. There was no improvement in the oxidant-antioxidant balance in colonic digesta of piglets fed IN10 and IN23 diets. Piglets fed IN10 diets had lower 1,N6-etheno-2’-deoxyadenosine excision activity in each colon segment, as compared with the control group. It was also reduced by the 2% and 3% IN23 diets in the proximal colon, while in the middle and distal colon by all IN23 diets. Feeding all IN10 and IN23 diets reduced 3,N4-etheno-2’-deoxycytidine and 8-oxo-deoxyguanosine excision activities in each colon segment. Feeding IN10 and IN23 diets neither decreased amine concentrations nor improved the oxidant-antioxidant balance in colonic digesta of piglets. However, both types of inulin efficiently reduced the activity of DNA repair enzymes.

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“…Inulin is non-digestible; therefore, passes throughout the small intestine and isfermented in the colon. Inulin may be able to feed and stimulate the growth and propagation of Bifi dobacteria species and other probiotics in the colon (Guarino et al 2017, Lightowler et al 2017, Lopes et al 2017, Valdés-Varela et al 2017, Vandeputte et al 2017.…”

Section: Introductionmentioning

confidence: 99%

Effects of inulin and fat percentage on the viability of Bifi dobacterium lactis Bb12 in chocolate milk

Pourjafar¹,

Ayareh²,

Karim³

et al. 2017

Biosci. Biotech. Res. Comm

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Bifi dobacterium genus has several benefi cial effectsas a probiotic bacterium. It has been demonstrated that Inulin and fat percentage of the probiotic product may modify the viability of this bacterium during storage time. The aim of this study was fi nding the best formulation of the chocolate milk regarding bacterial viability and sensory properties of the fi nal product. Four types of probiotic chocolate milk, with and without inulin in 1% fat samples, with and without inulin in 2% fat samples, were manufactured in duplicate. The count of probiotic cells during 12 days of storage in the refrigerator was assessed. In all samples, the viable cells of Bifi dobacterium lactis increased except 1% fat samples without inulin in which bacterial count decreased (not signifi cantly). The rate of change in the bacterial count was signifi cantly p<0.05 higher in the 2% fat chocolate milk both in with and without inulin samples (0.6 and 0.8) compared with 1% fat samples (0.5 and-0.4). Inulin inoculation had some effects on biochemical and organoleptic changes. After 12 days storage, the acceptability of 2% fat milk with probiotic and inuline was the highest. The results illustrated that the most signifi cant Bifi dobacterium lactis viability was in 2% fat chocolate milk. Adding inulin improved the viability of bacteria in 1% fat milk; however, ithad not any signifi cant effects on the viability of probiotic bacteria in 2% fat milk. In conclusion, 2% fat chocolate milk with inuline could be recommended to be the best group.

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Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon

Cited by 19 publications

References 51 publications

Mechanisms of Action of Prebiotics and Their Effects on Gastro-Intestinal Disorders in Adults

Mechanisms of Action of Prebiotics and Their Effects on Gastro-Intestinal Disorders in Adults

The effect of dietary level of two inulin types differing in chain length on biogenic amine concentration, oxidant-antioxidant balance and DNA repair in the colon of piglets

Effects of inulin and fat percentage on the viability of Bifi dobacterium lactis Bb12 in chocolate milk

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