Effect of iodide on total antioxidant status of human serum

Abstract

Free radicals and subsequent lipid peroxidation have been implicated in the pathogenesis of several degenerative and chronic diseases which are also treated frequently in spas. There are some data arising from previous studies which support an antioxidant or scavenging effect of iodide, being the essential ingredient of a therapeutically used local brine. The aim of the study was to test the antioxidant capacity of iodide in human serum. For this reason we measured the so-called Total Antioxidant Status determined by a colorimetric method, which reflects the protection against the attack of reactive oxygen species, including enzymic and non-enzymic antioxidants. Exogenous iodide applied as NaI, shows a significantly increased antioxidant capacity in comparison with NaCl at a concentration of 15 microM, which is quite comparable to the upper range of serum iodide levels achieved through balneo-therapeutical intervention. This result is in accordance with previous results from in vitro depolymerization experiments with hyaluronic acid. The antioxidant effect of 15 microM NaI has been found to be approaching the physiologically relevant concentration of ascorbic acid (50 microM).

The Extrathyronine Actions of Iodine as Antioxidant, Apoptotic, and Differentiation Factor in Various Tissues

“…Berking et al (37) demonstrated the antioxidant action of Iin polyps of the jellyfish Aurelia aurita, whereas Elstner and co-workers found this antioxidant effect in the rabbit eye (38). Micromolar amounts of Idecrease damage by free oxygen radicals, increase the total antioxidant status in human serum (39,40), and defend brain cells in rats from lipid peroxidation (41). Thyroxine and other iodothyronines act as antioxidants and inhibitors of lipid peroxidation after they are oxidized by hemoglobin and their iodine is released (42,43).…”

Iodine—A Potential Antioxidant and the Role of Iodine/Iodide in Health and Disease

“…It could be shown that iodide inhibits the free radical-induced depolymerisation of hyaluronic acid measured by its decrease of in viscosity. This antiradical effect has been shown significantly down to concentrations In vitro studies with human serum showed an increase in the total antioxidant status (TAS) by a colorimetric method at a concentration beginning with 0.7 µM iodide [9], as well as an increase in peroxidase activity [10]. Accordingly, there was a decrease in the peroxide level in this concentration range (Figure 3).…”

Anti‐peroxidation effect of guava (Psidium guajava Linn.) leaf soluble solids in vitro and in streptozotocin/ nicotinamide‐induced diabetic rats

“…The TAS assay has proven to be a relatively simple and useful method for estimating antioxidant capacity in serum 42. The assay is based on the scavenging effect of antioxidants on 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) radicals (ABTS + ) 43.…”