Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis

Stähli, Christoph; James-Bhasin, Mark; Hoppe, Alexander; Boccaccını, Aldo R.; Nazhat, Showan N.

doi:10.1016/j.actbio.2015.03.009

Cited by 81 publications

(44 citation statements)

References 59 publications

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“…Significant increase in vasculogenesis in all doped BrCs at 8 weeks post implantation in comparison to 4 weeks proves the positive effect of Si and its concentration on vasculogenesis. Although the complete mechanism governing the blood vessel formation in calcium phosphates are yet to be understood, it has been shown that Si upregulates the nitric oxide synthase and increases the VEGF production in bioglasses and calcium silicate materials in dose dependent manner through enhancement of vascular endothelial growth factor receptor (VEGFR-2) and basic fibroblast growth factor receptor (bFGF) in endothelial cells [41,42,43]. In addition, upregulation of endothelial gene markers by human dental pulp stromal cells (HDPSCs) shows the efficacy of bioglasses in regeneration of vascularized bone substitutes in combination of HDPSCs [44].…”

Section: Discussionmentioning

confidence: 99%

Effects of silicon on osteoclast cell mediated degradation, in vivo osteogenesis and vasculogenesis of brushite cement

2015

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Calcium phosphate cements (CPCs) are being widely used for treating small scale bone defects. Among the various CPCs, brushite (dicalcium phosphate dihydrate, DCPD) cement is widely used due to its superior solubility and ability to form new bone. In the present study, we have studied the physical, mechanical, osteoclast-like-cells differentiation and in vivo osteogenic and vasculogenic properties of silicon (Si) doped brushite cements. Addition of Si did not alter the phase composition of final product and regardless of Si level, all samples included β-tricalcium phosphate (β-TCP) and DCPD. 1.1 wt. % Si addition increased the compressive strength of undoped brushite cement from 4.78±0.21 MPa to 5.53±0.53 MPa, significantly. Cellular activity was studied using receptor activator of nuclear factor κβ ligand (RANKL) supplemented osteoclast-like-cells precursor RAW 264.7 cell. Phenotypic expressions of the cells confirmed successful differentiation of RAW264.7 monocytes to osteoclast-like-cells on undoped and doped brushite cements. An increased activity of osteoclast-like cells was noticed due to Si doping in the brushite cement. An excellent new bone formation was found in all cement compositions, with significant increase in Si doped brushite samples as early as 4 weeks post implantation in rat femoral model. After 4 weeks of implantation, no significant difference was found in blood vessel formation between the undoped and doped cements, however, a significant increase in vasculgenesis was found in 0.8 and 1.1 wt. % Si doped brushite cements after 8 weeks. These results show the influence of Si dopant on physical, mechanical, in vitro osteoclastogenesis and in vivo osteogenic and vasculogenic properties of brushite cements.

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Section: Discussionmentioning

confidence: 99%

Effects of silicon on osteoclast cell mediated degradation, in vivo osteogenesis and vasculogenesis of brushite cement

2015

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“…It has been confirmed that Cu is able to promote angiogenesis by stabilizing the expression of hypoxia-inducible factor (HIF-1α) and secretion of VEGF of bone marrow stromal cells, thus artificially mimicking hypoxia, which plays an important role in recruitment and differentiation of cells and in blood vessel formation [11][12][13]. Besides, Cu ions may also be involved in extracellular matrix (ECM) degradation and remodeling [14].…”

Section: Introductionmentioning

confidence: 98%

Copper-containing mesoporous bioactive glass promotes angiogenesis in an in vivo zebrafish model

et al. 2018

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Mesoporous bioactive glasses (MBGs) with high specific surface area, well-ordered pores, large pore volumes and controllable amount of ions are interesting to develop controlled drug delivery systems for bone tissue regeneration. Copper (Cu) incorporation to the basic SiO-CaO-PO composition has attracted high interest due to its multifunctional biological properties. Promotion of angiogenesis is one of these properties, which can be integrated to the biomaterial with lower cost and higher stability when compared with growth factors. This work reports the synthesis and characterization of Cu-containing MBG evaluating its angiogenic properties in the subintestinal vessel zebrafish assay. This transgenic in vivo assay is merging as an alternative model providing short-time consuming protocols and facilities during pro-angiogenic drug screenings. The report shows that the ionic products of this MBG material delivered to the zebrafish incubation media significantly enhance angiogenesis in comparison with control groups. Besides, results indicate Cu ions may exhibit a synergic effect with Si, Ca, and P ions in angiogenesis stimulation both in vitro and in vivo. To our knowledge, this is the first time that zebrafish in vivo assays are used to evaluate angiogenic activity of ionic dissolution products from MBG materials.

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“…DMEM is usually used as a mimetic cell culture environment to assess the degradability of materials, since dissolution products in DMEM could be utilized directly to evaluate the biocompatibility and osteogenic activity of materials. 50 However, DMEM intrinsically contains abundant levels of potassium, calcium, and amino acids, leading to possible interaction with the tested material over time. 51 In this study, the assessment medium (DMEMWater mixture) contained Ca 2+ ions at a concentration of $6.5 mg mL À1 .…”

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confidence: 99%

Timing of calcium nitrate addition affects morphology, dispersity and composition of bioactive glass nanoparticles

et al. 2016

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Bioactive glass nanoparticles (BGN) are promising materials for a number of biomedical applications. Many parameters related to the synthesis of BGN using sol-gel methods can affect their characteristics. In this study, the influence of timing of calcium nitrate (calcium precursor) addition during processing on BGN characteristics was investigated. The results showed that the addition timing could affect the morphology, dispersity and composition of BGN. With delayed addition of calcium nitrate, larger, more regular and better dispersed BGN could be synthesized while the gap between nominal and actual compositions of BGN was widened. However, the addition timing had no significant influence on structural characteristics, as BGN with different addition-timing of calcium nitrate exhibited similar infrared spectra and amorphous nature. The results also suggested that monodispersed BGN could be synthesized by carefully controlling the addition of calcium nitrate. The synthesized monodispersed BGN could release Si and Ca ions continuously for up to at least 14 days. They also showed in vitro bioactivity and non-cytotoxicity towards rat bone marrow-derived mesenchymal stem cells (rBMSCs). In conclusion, the timing of calcium precursor addition is an essential parameter to be considered when producing BGN which should exhibit monodisperse characteristics for biomedical applications.

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Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis

Cited by 81 publications

References 59 publications

Effects of silicon on osteoclast cell mediated degradation, in vivo osteogenesis and vasculogenesis of brushite cement

Effects of silicon on osteoclast cell mediated degradation, in vivo osteogenesis and vasculogenesis of brushite cement

Copper-containing mesoporous bioactive glass promotes angiogenesis in an in vivo zebrafish model

Timing of calcium nitrate addition affects morphology, dispersity and composition of bioactive glass nanoparticles

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