Effect of ions on Mohr method for chloride determination

Sheen, H. T.; Kähler, Hendrik

doi:10.1021/ac50127a004

Cited by 60 publications

(36 citation statements)

References 2 publications

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“…For comparison, synthetic medium AS100 (Vonshak, 1986) modified with the addition of 0.2 M NaCl was also used to determine the microalgae optimal growth. The initial concentration of seawater was determined by Mohr method (Sheen and Kahler, 1938), which uses chromate ions as an indicator in the titration of chloride ions with silver nitrate standard solution.…”

Section: Methodsmentioning

confidence: 99%

Modeling Growth of Microalgae <i>Dunaliella Salina</i> under Different Nutritional Conditions

Jesús¹

2010

American Journal of Biochemistry and Biotechnology

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Problem statement:The aim of this study was to find the empirical model that describes the growth kinetics of Dunaliella salina, with low production cost and to estimate parameters of this model. Approach: In this study the strain of D. salina UTEX 200 was cultivated in seawater (0.5 M NaCl) at room temperature with agitation of 150 rpm and luminous intensity of 60 mmols.m-2.s-1. The synthetic medium AS100 (0.2 M NaCl) was used in this study for comparison purposes and in order to determine the optimal growth of the microalgae. Kinetics of growth and β-carotene production was determined in a period of 15 days. Results: After the analysis of the behavior graphic, an unstructured model was used for describing the cell growth (logistic model). It was observed that the model was well adjusted to experimental data for the two conditions of analysis. It was observed that alga produces carotenoids under conditions of stress, in which the cell division are retarded. In the case of cells grown in seawater (higher salt concentration), the cell growth was lower but the concentration of β-carotene was higher. Conclusion: In general, these results suggest that D. salina presents higher potential for β-carotene accumulation and that high salinity decreases cellular concentration (measured by the parameter Xm of the kinetic model proposed); however there is an increase in β-carotene production.

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Section: Methodsmentioning

confidence: 99%

Modeling Growth of Microalgae <i>Dunaliella Salina</i> under Different Nutritional Conditions

Jesús¹

2010

American Journal of Biochemistry and Biotechnology

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“…The salt concentration of the cheese crackers was determined by the Mohr method [8] with slight modification; titration with silver nitrate. The cheese cracker samples were evaluated at week 1.…”

Section: Salt Concentration Determinationmentioning

confidence: 99%

“…The solution was then titrated with 0.1 N silver nitrate until the solution changed color (bright red). The percentage of salt was determined using the equation provided by Sheen and Kahler [8].…”

Section: Salt Concentration Determinationmentioning

confidence: 99%

Influence of Nano-Spray Dried Sodium Chloride on the Physicochemical Characteristics of Surface-Salted Cheese Crackers

Moncada¹,

Astete²,

Sabliov³

et al. 2017

FNS

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Particle size reduction of salt to submicron range increases its surface area resulting in increased saltiness perception. The objective was to evaluate the incorporation of nano-spray dried sodium chloride on the physicochemical characteristics of surface-salted cheese crackers. The sodium chloride solution (3% w/w) was sprayed through a 4-μm nozzle with 125 L/min air flow, 38 kPa pressure, 95˚C head temperature, and 90% spray to form the smallest submicrosalt particles. The cheese cracker treatments consisted of 3 different salt sizes (regular, microsalt and nano-spray dried salt) and 3 different concentrations (2%, 1.5% and 1%). The 9 (3 sizes × 3 concentrations) cheese cracker treatments were tested for salt concentration and sodium content at week 1. Water activity ( w a ), texture-fracturability, and color were determined at week 1 and 4 months of storage. The w a and the * L , * b , * C and * h values in all treatments increased from 1 week to 4 months. The use of nano-spray dried salt on surface-salted cheese crackers allowed for a reduction of 25% -50% of salt content without affecting the physicochemical attributes.

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“…By extracting the liquid phase of the saturation paste under partial vacuum estimates of solution concentrations of Na + , K + , Ca 2+ , Mg 2+ , Cl -, HBO 3 , NO 3 -, SO 4 2-, Mn 2+ , SeO 4 2-, HCO 3 -, CO 3 2-can be determined. The contents of various ions were measured Cl -by titration with AgNO 3 and Mg 2+ by atomic absorption spectrophotometer, Na + by flame spectrophotometry and Ca 2+ by complexometry method using EDTA [15] are summarized in Table 1.…”

Section: International Letters Of Natural Sciences Vol 60 39mentioning

confidence: 99%

An Improved Procedure of the Metagenomic DNA Extraction from Saline Soil, Sediment and Salt

Kashi

2016

ILNS

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Abstract.A new modified protocol has been developed for extracting pure community inhibitorsfree DNA from saline soils, sediments and salts. Amplification of DNA from soil and sediment is often inhibited by copurified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to saline samples. Using a widely used a newly modified direct DNA extraction method proposed in this report, DNA was extracted from samples of Urmia Lake in diverse geological location in Iran and quantity of the DNA were examined. We developed an improved method to extract DNA include the combination of physical, chemical and mechanical lysis methods from saline samples. In the earlier reports, skim milk as an adsorption competitor was added to buffer DNA extract. In current study, we added skim milk to buffer DNA extraction. The results showed that skim milk was useful as an additive for extract DNA from saline samples. This method is applicable to molecular community analysis of saline samples which strongly adsorb DNA. The methods appear to have wide applicability in investigating molecular diversity and exploring functional genes from the total DNA. The extracted DNA was used to successfully amplify 16SrRNA region and functional genes. The amplicons were suitable for further applications such as diversity based analysis by denaturing gradient gel electrophoresis (DGGE) and cloning library.

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Effect of ions on Mohr method for chloride determination

Cited by 60 publications

References 2 publications

Modeling Growth of Microalgae <i>Dunaliella Salina</i> under Different Nutritional Conditions

Modeling Growth of Microalgae <i>Dunaliella Salina</i> under Different Nutritional Conditions

Influence of Nano-Spray Dried Sodium Chloride on the Physicochemical Characteristics of Surface-Salted Cheese Crackers

An Improved Procedure of the Metagenomic DNA Extraction from Saline Soil, Sediment and Salt

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