Effect of Iron Oxide Nanoparticle Shape on Doxorubicin Drug Delivery Toward LNCaP and PC-3 Cell Lines

Nizamov, Timur R.; Garanina, Anastasiia S.; Grebennikov, I. S.; Zhironkina, Oxana A.; Strelkova, Olga; Alieva, Irina B.; Kireev, Igor I.; Abakumov, Maxim; Savchenko, Alexander; Majouga, Alexander G.

doi:10.1007/s12668-018-0502-y

Cited by 12 publications

(6 citation statements)

References 39 publications

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“…Indeed, the percentage of cell survival is 17.2 ± 4.3% for free DOX, while DOX-loaded nanoparticles show cell survival of 37.1 ± 9.5% in the studied range of concentration. This difference could be explained by the different behavior of the free and loaded DOX inside the cells [ 56 , 57 ]. DOX shows antiproliferative activity against many tumor cells and has the potential to permeate through the cell membrane very easily thanks to its hydrophobic nature.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine Modified Superparamagnetic Iron Oxide Nanoparticles as Multifunctional Nanocarrier for Targeted Prostate Cancer Treatment

et al. 2019

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Polydopamine (pDA)-modified iron oxide core-shell nanoparticles (IONPs) are developed and designed as nanovectors of drugs. Reactive quinone of pDA enhances the binding efficiency of various biomolecules for targeted delivery. Glutathione disulfide (GSSG), an abundant thiol species in the cytoplasm, was immobilized on the pDA-IONP surface. It serves as a cellular trigger to release the drug from the nanoparticles providing an efficient platform for the drug delivery system. Additionally, GSSG on the surface was further modified to form S-nitrosoglutathione that can act as nitric oxide (NO) donors. These NPs were fully characterized using a transmission electronic microscopy (TEM), thermogravimetric analysis (TGA), dynamic light scattering (DLS), zeta potential, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR) and UV-vis spectroscopies. Doxorubicin (DOX) and docetaxel (DTX) are two anticancer drugs, which were loaded onto nanoparticles with respective loading efficiencies of 243 and 223 µmol/g of IONPs, calculated using TGA measurements. DOX release study, using UV-vis spectroscopy, showed a pH responsive behavior, making the elaborated nanocarrier a potential drug delivery system. (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium (MTS) and apoptosis assays were performed on PC3 cell lines to evaluate the efficiency of the developed nanocarriers. These nanoparticles thus can prove their worth in cancer treatment on account of their easy access to the site and release of drug in response to changes to internal parameters such as pH, chemicals, etc.

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Section: Resultsmentioning

confidence: 99%

Polydopamine Modified Superparamagnetic Iron Oxide Nanoparticles as Multifunctional Nanocarrier for Targeted Prostate Cancer Treatment

et al. 2019

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“…The lack of fluorescence is due to the quenching of DOX on the AuNP surface due to the closely arranged or π-stacked DOX molecules [ 50 ]. In addition, the proximity of DOX to the AuNP core is another possible reason for its fluorescence quenching through energy transfer from DOX to the gold core [ 51 , 52 ]. Acidification to a pH of 4.5 caused the DOX–hydrazone bond to be cleaved, and an initial rapid release of DOX was observed at 15 min ( Figure 5 A).…”

Section: Resultsmentioning

confidence: 99%

Targeted Doxorubicin-Loaded Dendronized Gold Nanoparticles

Dockery

Daniel

2023

Pharmaceutics

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Dendronized nanoparticles, also called nanoparticle-cored dendrimers, combine the advantages of nanoparticles and dendrimers. These very stable and polyvalent nanoparticles can be used for diverse applications. One such application is drug delivery, because the dendrons can enhance the density of the payload. In this report, we describe the design of multifunctional gold nanoparticles (AuNPs) coated with poly(propylene imine) (PPI) dendrons that contain both prostate cancer active targeting and chemotherapeutic drugs. The PPI dendron is a good candidate for the design of drug delivery vehicles because of its ability to induce a proton sponge effect that will enhance lysosomal escape and intracellular therapeutic delivery. The chemotherapeutic drug used is doxorubicin (DOX), and it was linked to the dendron through a hydrazone acid-sensitive bond. Subsequent acidification of the AuNP system to a pH of 4–5 resulted in the release of 140 DOX drugs per nanoparticles. In addition, the PPI dendron was conjugated via “click” chemistry to an EphA2-targeting antibody fragment that has been shown to target prostate cancer cells. In vitro cell viability assays revealed an IC50 of 0.9 nM for the targeted DOX-bearing AuNPs after 48 h incubation with PC3 cells. These results are very promising upon optimization of the system.

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“…PC-3 and LNCaP viability ratios were also significantly different from each other. Though there have been numerous articles on these cell lines and combination studies using radiation and/or chemotherapeutic drugs along with hyperthermia, none of the studies used MNPs as the heat mediator in vitro [61][62][63][64][65][66][67][68][69]. Instead, they employed different methods to reach hyperthermic levels, such as using an incubator or hot water [70,71].…”

Section: Magnetic Fluid Hyperthermiamentioning

confidence: 99%

Development of handheld induction heaters for magnetic fluid hyperthermia applications and in-vitro evaluation on ovarian and prostate cancer cell lines

Castro-Torres

Mendéz²,

Torres‐Lugo³

et al. 2023

Biomed. Phys. Eng. Express

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Abstract— Objective: Magnetic fluid hyperthermia (MFH) is a still experimental technique found to have a potential application in the treatment of cancer. The method aims to reach around 41–47°C in the tumor site by exciting magnetic nanoparticles with an externally applied alternating magnetic field (AMF), where cell death is expected to occur. Applying AMFs with high spatial resolution is still a challenge. The AMFs from current and prospective MFH applicators cover relatively large areas; being not suitable for patients having metallic implants near the treatment area. Thus, there will be a clinical need for smaller magnetic field applicators. To this end, a laparoscopic induction heater (LIH) and a transrectal induction heater (TRIH) were developed. Methods: Miniature “pancake” coils were wound and inserted into 3D printed enclosures. Ovarian (SKOV-3, A2780) and prostate (PC-3, LNCaP) cancer cell lines were used to evaluate the instruments’ capabilities in killing cancer cells in vitro, using Synomag®-D nanoparticles as the heat mediators. NIH3T3 normal cell lines were also used with both devices to observe if these cells tolerated the conditions applied. Results: Magnetic field intensities reached by the LIH and TRIH were 42.6 kA/m at 326 kHz and 26.3 kA/m at 303 kHz, respectively. Temperatures reached in the samples were 41°C by the LIH and 43°C by the TRIH. Both instruments successfully accomplished killing cancer cells, with minimal effects on normal cells. Conclusion: This work presents the first line of handheld medical induction heaters and have the potential to be a complement to existing cancer therapies. Significance: These instruments could enable the development of MFH modalities that will facilitate the clinical translation of this thermal treatment.

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Effect of Iron Oxide Nanoparticle Shape on Doxorubicin Drug Delivery Toward LNCaP and PC-3 Cell Lines

Cited by 12 publications

References 39 publications

Polydopamine Modified Superparamagnetic Iron Oxide Nanoparticles as Multifunctional Nanocarrier for Targeted Prostate Cancer Treatment

Polydopamine Modified Superparamagnetic Iron Oxide Nanoparticles as Multifunctional Nanocarrier for Targeted Prostate Cancer Treatment

Targeted Doxorubicin-Loaded Dendronized Gold Nanoparticles

Development of handheld induction heaters for magnetic fluid hyperthermia applications and in-vitro evaluation on ovarian and prostate cancer cell lines

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