Effect of iron oxide nanoparticles on the permeability properties of Sf21 cells

Principles and advances of cell cryopreservation

“…并进一步将温度传感器集成到微通道内, 实现了 细胞尺度温度的精确控制, 有效降低了温差带来的参 数测定误差(图3A) [50] . 本团队随后将该方法应用于猪 髂动脉内皮细胞 [52] 、猪脂肪间充质干细胞 [53] 、SF21 细胞 [54] 、HepG2细胞等 [55] 多种不同类型细胞的渗透 性研究. 2017年, 本团队 [56] 提出了一种双层三明治结 构微灌流腔设计方法, 不仅实现了挡板结构对人卵母 细胞自然限位, 而且通过对微通道内部混合过程的建 模实现了对细胞外浓度场和细胞尺度浓度场(细胞膜 表面瞬态浓度分布)的同步精确刻画, 建立了人卵母细 胞渗透性研究的通用工具(图3A).…”

Current research on ecotoxicity of metal-based nanoparticles: from exposure pathways, ecotoxicological effects to toxicity mechanisms

“…The cell membrane is a barrier for the exchange of substances inside and outside the cell, preventing harmful substances from entering the cell and protecting the internal structure of the cell. Studies have shown that metal-based NPs may cause direct damage to the cell membrane, resulting in altered cell membrane permeability ( 93 ), the disruption of cell membrane integrity ( 94 ), and the alteration of cell membrane structure ( 95 ), among others. For example, zinc oxide NPs induce toxicity by affecting cell wall integrity pathways, mitochondrial function, and lipid homeostasis in Saccharomyces cerevisiae ( 96 ).…”

Applying nanotechnology to cryopreservation studies: Status and future

“…At slow cooling rates below the optimal rate, cell injury results from "solution effects". At high cooling rates above the optimal rate, the formation of intracellular ice leads to lethal cell injury 粒) [38] ; (b) 不同温度下, 纳米颗粒对PIECs细胞的水渗透系数Lp的影响 [38] ; (c) 不同温度下, 不同浓度纳米颗粒对Sf21细胞的水渗透系数Lp的影 响 [19] Figure 3 (Color online) Effect of nanoparticles on cell volume response and membrane hydraulic permeability parameters. (a) The osmotic volume responses of PIECs (an osmoticshift from PBS solution to 3×PBS+0.01% HA-nanoparticles) [38] ; (b) effect of different temperature and nanoparticles on cell membrane hydraulic conductivity (Lp) of PIECs [38] ; (c) effect of different temperature and concentrations of nanoparticles on Lp values of Sf21 cells [19] 图 4 纳米颗粒的种类及载液量(η=2%(a)及20%(b))对细胞降温速率响应的影响 [41]…”