Effect of irreversible electroporation on cancer cells

Sundararajan, Raji; Rajendran, Ramya Lakshmi; Shahid, Sajan S.; Santosh, D. K.; Radhakrishnan, Snehalatha; Priyadarshan, K.; Varsha, S.; Kumar, Umesh; Ramachandran, Raja Prabu; Kannan, Sankaranarayanan

doi:10.1109/ceidp.2011.6232622

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…Sankaranarayanan et al report irreversible electroporation in chick embryo fibroblast cells exposed to 1 and 8 pulses at 1.2 kV/cm with 100 μsPEF. 44 Hanna et al show reduced cell viability in the Chinese hamster DC-3F cell line after exposure to 1 pulse of 100 μsPEF at 1.4–2.0 kV/cm, although they show that human amniotic mesenchymal stromal stem cell (haMSC) mortality is not reduced after exposure to a similar, single 100 μsPEF at 2.0 kV/cm. 14 Thus, different cell types are expected to respond differently to a given pulse parameter set of μsPEF exposure.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Cell Viability and Migration of Primary Bovine Annular Fibrosus Fibroblast-like Cells Induced by Microsecond Pulsed Electric Field Exposure

Atsu,

Mowen,

Thompson

2023

ACS Omega

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This study is the first to report the enhancement of cell migration and proliferation induced by in vitro microsecond pulsed electric field (μsPEF) exposure of primary bovine annulus fibrosus (AF) fibroblast-like cells. AF primary cells isolated from fresh bovine intervertebral disks (IVDs) are exposed to 10 and 100 μsPEFs with different numbers of pulses and applied electric field strengths. The results indicate that 10 μs-duration pulses induce reversible electroporation, while 100 μs pulses induce irreversible electroporation of the cells. Additionally, μsPEF exposure increased AF cell proliferation up to 150% while increasing the average migration speed by 0.08 μm/min over 24 h. The findings suggest that the effects of PEF exposure on cells are multifactorial� depending on the duration, intensity, and number of pulses used in the stimulation. This highlights the importance of optimizing the μsPEF parameters for specific cell types and applications. For instance, if the goal is to induce cell death for cancer treatment, then high numbers of pulses can be used to maximize the lethal effects. On the other hand, if the goal is to enhance cell proliferation, a combination of the number of pulses and the applied electric field strength can be tuned to achieve the desired outcome. The information gleaned from this study can be applied in the future to in vitro cell culture expansion and tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Enhanced Cell Viability and Migration of Primary Bovine Annular Fibrosus Fibroblast-like Cells Induced by Microsecond Pulsed Electric Field Exposure

Atsu,

Mowen,

Thompson

2023

ACS Omega

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“…The A549 cell line was fi rst developed in 1972 by Giard et al, through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58-year-old Caucasian male [21]. They are human alveolar basal epithelial cells, are squamous in nature and responsible for the diffusion of substances, such as water and electrolytes, across the alveoli of lungs.…”

Section: Lung Adenocarcinoma (A549)mentioning

confidence: 99%

“…After electroporation, cell viability was determined by Trypan Blue Exclusion assay, as explained in previous publications [19,21,23]. In the case of CEFs, the viability was found using phase contrast microscopy.…”

Section: Viability Calculationsmentioning

confidence: 99%

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Irreversible electroporation: a drug-free cancer treatment

Sundararajan¹,

Salameh²,

Camarillo³

et al. 2014

Electroporation-Based Therapies for Cancer

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“…e effects during electroporation could be reversible or irreversible depending on the treatment intensity that causes damage to the membrane of a living cell [14][15][16][17][18]. In the irreversible electroporation, the cell loses its homeostasis and eventually dies if and only if the strength of the electric field applied is sufficiently intense [19].…”

Section: Introductionmentioning

confidence: 99%

Compact High-Voltage Pulse Generator for Pulsed Electric Field Applications: Lab-Scale Development

Kasri

Piah

Adzis

2020

Journal of Electrical and Computer Engineering

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Square wave pulses have been identified as more lethal compared to exponential decay pulses in PEF applications. This is because of the on-time which is longer causes a formidable impact on the microorganisms in the food media. To have a reliable high-voltage pulse generator, a technique of capacitor discharge was employed. Four units of capacitor rated 100 μF 1.2 kV were connected in series to produce 25 μF 4.8 kV which were used to store the energy of approximately 200 J. The energy stored was discharged via HTS 181-01-C to the load in the range of nano to microseconds of pulse duration. The maximum voltage applied was limited to 4 kV because it is a lab-scale project. The electrical circuit diagram and the development procedure, as well as experimental results, are presented.

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Effect of irreversible electroporation on cancer cells

Cited by 6 publications

References 10 publications

Enhanced Cell Viability and Migration of Primary Bovine Annular Fibrosus Fibroblast-like Cells Induced by Microsecond Pulsed Electric Field Exposure

Enhanced Cell Viability and Migration of Primary Bovine Annular Fibrosus Fibroblast-like Cells Induced by Microsecond Pulsed Electric Field Exposure

Irreversible electroporation: a drug-free cancer treatment

Compact High-Voltage Pulse Generator for Pulsed Electric Field Applications: Lab-Scale Development

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