Effect of Ischemia and Infarction on Regional Content of Adenine Nucleotides and Derivatives in Canine Left Ventricle¹

Abstract

Samples of myocardium from four areas of ischemic and infarcted canine ventricle were examined over a 20-day period for content of the three adenine nucleotides as well as inosine, hypoxanthine, adenosine, and inosine monophosphate. The adenosine triphosphate (ATP) content of central and peripheral areas within the infarct fell to 11% and 8% of control, respectively, 1 day after coronary occlusion. The total adenine nucleotide (TAN) content in these areas fell to 17% of control and showed no significant recovery during the period of study. In the functional myocardium immediately surrounding the infarct the ATP content was depressed to 58% of control after 1 day, and the TAN content was also depressed. In the healthy myocardium near the apex, the ATP content was significantly depressed only at the 3-day sample period. Adenine nucleotide derivatives were detected only at 30 min in the central ischemic area. The absence of the nucleoside and nucleobase compounds formed during adenine nucleotide degradation is attributed to their high membrane diffusibility. Loss of these compounds is considered a contributing factor in the prolonged depression of adenine nucleotide content in both ischemic and non-ischemic regions.

Effect of Inosine on Ventricular Regional Perfusion and Infarct Size after Coronary Occlusion

“…Consequently, there is a transient but substantial increase in myocardial content of nucleotide derivatives (14,15,25). Studies have demonstrated important effects o f these derivatives on the myocardium and coronary vasculature (28,30).…”