Effect of isoflavone from Flemingia vestita (Fabaceae) on the Ca2+ homeostasis in Raillietina echinobothrida, the cestode of domestic fowl

Das, Bidyadhar; Tandon, Veena; Saha, Nirmalendu

doi:10.1016/j.parint.2005.08.002

Cited by 17 publications

(17 citation statements)

References 25 publications

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“…In corroboration with the decrease of Ca 2+ concentration in the tissue, there was also a significant increase of Ca 2+ concentration in the incubation medium, indicating thereby an increased efflux of Ca 2+ into the medium during the treatments. Similar types of observations were also reported by Prichard et al (1982), Martinez et al (1992), Das et al (2006), and Dasgupta et al (2013) in helminth parasites. Simultaneously, our results also showed an increase of Na 2+ and K + concentration in the medium as also observed by Lalchhandama et al (2007) in R. echinobothrida.…”

Section: Discussionsupporting

confidence: 89%

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Sennaleaf extracts induced Ca⁺²homeostasis in a zoonotic tapewormHymenolepis diminuta

Roy

Kundu

Lyndem

2016

Pharmaceutical Biology

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Context Plants and plant products have been used in traditional medicine as anthelmintic agents in human and veterinary medicine. Three species of Senna plant, S. alata (L), S. alexandrina (M) and S. occidentalis (L.) Link (Fabaceae) have been shown to have a vermicidal/vermifugal effect on a zoonotic tapeworm Hymenolepis diminuta (Rudolphi) (Cyclophyllidean). Objective The present study validates the mode of action of these Senna plants on the parasite. The alcoholic leaf extract was determined to obtain information on the intracellular free calcium concentration level. Materials and methods Hymenolepis diminuta was maintained in Sprague-Dawley rat model for 2 months. Live parasites collected from infected rat intestine were exposed to 40 mg/mL concentration of each plant extracts prepared in phosphate buffer saline at 37 C, till parasite gets paralyzed. The rate of efflux of calcium from the parasite tissue to the medium and the level of intracellular Ca 2+ concentration were determined by an atomic absorption spectroscopy. Results This study revealed that exposure of the worms to the plant extract leads to disruption in intracellular calcium homeostasis. A significant increase (44.6% and 25%) of efflux in Ca 2+ from the tissue to the incubated medium was observed. Senna alata showed high rate of efflux (5.32 mg/g) followed by S. alexandria and S. occidentalis (both 4.6 mg/g) compared with control (3.68 mg/g). Discussion and conclusion These results suggest that leaf extracts caused membrane permeability to Ca 2+ after vacuolization of the tegument under stress and the extracts may contain compound that can be used as a chemotherapeutic agent. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 89%

“…These observations were also reported by Das et al (2006). This may be due to the fact that all these trace elements which are responsible for muscular contraction may have followed the same pattern.…”

Section: Discussionsupporting

confidence: 77%

Sennaleaf extracts induced Ca⁺²homeostasis in a zoonotic tapewormHymenolepis diminuta

Roy

Kundu

Lyndem

2016

Pharmaceutical Biology

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“…[All scale bar= 100 μm] junction by inhibiting the AChE (Bryant and Behm 1989). Flaccid paralysis of R. echinobothrida due to exposure to the phytochemicals as observed in the present study may be a result of neuromuscular blockage and a persistent muscle contraction as also observed earlier in R. echinobothrida exposed to genistein, an active principle of Flemingia vestita (Das et al 2006). Anthelmintic drugs levamisole, pyrantel, and morantel are revealed to be nicotinic receptor agonists, which elicit spastic muscle paralysis due to prolonged activation of the excitatory nicotinic acetylcholine receptors on the muscles of body wall (Martin et al 2005).…”

Section: Discussionsupporting

confidence: 83%

“…A change in Ca 2+ concentration or their capacitative entry to cells in response to agonists and shear stress is key for several endothelial cell functions (Tran et al 2000). Das et al (2006) observed occurrence of flaccid paralysis due to rapid muscle contractions and changes in the Ca 2+ homeostasis in R. echinobothrida treated with crude peel extract of F. vestita by the supplement action of elevated cGMP level.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol- and α-viniferin-induced alterations of acetylcholinesterase and nitric oxide synthase in Raillietina echinobothrida

Giri

Roy

2015

Parasitol Res

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Phytostilbenes, like resveratrol and α-viniferin, which occur mainly in the plants and belong to the families Cyperaceae, Vitaceae, and Gnetaceae are extensively popular for their medicinal and nutritional properties. In Northeast India, the Jaintia tribes consume these phytochemicals through aqueous extract of the medicinal plant Carex baccans to control helminthiasis. The present study aimed to investigate the inhibitory effect of the phytochemicals on neurotransmitters and its related enzymes in helminth parasite Raillietina echinobothrida. Viability of the parasites exposed to the phytostilbenes and extent of inhibition of cholinergic and nitrergic enzymes were evaluated in comparison to reference anthelmintic drug praziquantel and two known enzyme inhibitors, namely Nω-nitro-L-arginine and pyridostigmine. On exposure to resveratrol, α-viniferin, and reference drug praziquantel, the parasites ceased movement at 9.37, 11.38, and 0.24 h followed by death at 23.65, 34.13, and 1.87 h, respectively. Exposed parasites also showed a significant decrease in the activity of acetylcholinesterase (46.101, 65.935, and 63.645%) and nitric oxide synthase (61.241, 55.046, and 29.618%) in comparison to the controls. In addition, a decreased trend in nitric oxide (NO) level was also detected in the tissue of different phytochemical-exposed parasites compared to control. The present study suggests that anthelmintic potential of both the phytochemicals is mediated through inhibition of two vital enzymes which play diverse role in intracellular communications through neuromuscular system.

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“…As revealed by earlier studies, the concentration of Mg 2 + in R. echinobothrida tissue is about 1400 μg/g dry tissue weight while Mn 2 + is below the level of detection (Das et al 2006). Although the concentration of Mg 2 + is much higher than that of Mn 2 + in the tissue of M. dubius and H. diminuta, there was no activity observed when Mg 2 + was used Wilkes et al 1981).…”

Section: Discussionmentioning

confidence: 78%

Purification and characterization of phosphoenolpyruvate carboxykinase fromRaillietina echinobothrida, a cestode parasite of the domestic fowl

et al. 2012

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Phosphoenolpyruvate carboxykinase (PEPCK, EC 4.1.1.32) is an essential regulatory enzyme of glycolysis in helminths in contrast to its role in gluconeogenesis in their host. Previously we have reported that phytochemicals from Flemingia vestita (Family: Fabaceae), genistein in particular, have vermifugal action and are known to affect carbohydrate metabolism in the cestode, Raillietina echinobothrida. In order to determine the functional differences of PEPCK from the parasite and its avian host (Gallus domesticus), we purified the parasite enzyme apparently to homogeneity, and characterized it. The native PEPCK is a monomer with a subunit molecular weight of 65 kDa. The purified enzyme displayed standard Michaelis-Menten kinetics with Km value of 42·52 μM for its substrate PEP. The Ki for the competitive inhibitors GTP, GMP, ITP and IMP for the carboxylation reaction were determined and discussed. In order to identify putative modulators from plant sources, phytochemicals from F. vestita and Stephania glabra were tested on the purified PEPCK, which resulted in alteration of its activity. From our results, we hypothesize that PEPCK may be a potential target site for anthelmintic action.

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Effect of isoflavone from Flemingia vestita (Fabaceae) on the Ca2+ homeostasis in Raillietina echinobothrida, the cestode of domestic fowl

Cited by 17 publications

References 25 publications

Sennaleaf extracts induced Ca⁺²homeostasis in a zoonotic tapewormHymenolepis diminuta

Sennaleaf extracts induced Ca⁺²homeostasis in a zoonotic tapewormHymenolepis diminuta

Resveratrol- and α-viniferin-induced alterations of acetylcholinesterase and nitric oxide synthase in Raillietina echinobothrida

Purification and characterization of phosphoenolpyruvate carboxykinase fromRaillietina echinobothrida, a cestode parasite of the domestic fowl

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Effect of isoflavone from Flemingia vestita (Fabaceae) on the Ca2+ homeostasis in Raillietina echinobothrida, the cestode of domestic fowl

Cited by 17 publications

References 25 publications

Sennaleaf extracts induced Ca+2homeostasis in a zoonotic tapewormHymenolepis diminuta

Sennaleaf extracts induced Ca+2homeostasis in a zoonotic tapewormHymenolepis diminuta

Resveratrol- and α-viniferin-induced alterations of acetylcholinesterase and nitric oxide synthase in Raillietina echinobothrida

Purification and characterization of phosphoenolpyruvate carboxykinase fromRaillietina echinobothrida, a cestode parasite of the domestic fowl

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Sennaleaf extracts induced Ca⁺²homeostasis in a zoonotic tapewormHymenolepis diminuta

Sennaleaf extracts induced Ca⁺²homeostasis in a zoonotic tapewormHymenolepis diminuta