Effect of isolation temperature on the characteristics of extracellular proteases produced by Antarctic bacteria

Vázquez, Susana; Cormack, Walter P. Mac

doi:10.3402/polar.v21i1.6474

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…When the 105 strains were cultured in the liquid screening medium, as many as 85 strains did not produce enough proteases for inhibition analysis. Similar difficulties applying this screening technique to Antarctic bacteria have been previously described [9]. Therefore, only 20 strains were subjected to inhibition analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Investigating the microbial communities and their functions in coastal sediments is vital to elucidating the biogeochemical cycles in the Southern Ocean. There have been some reports aimed at the proteases or protease-producing bacteria in Antarctic marine sediment [7-9]; however, the diversity of protease-producing bacteria in these permanently low-temperature sediments has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Both the Cultivable Protease-Producing Bacteria and Bacterial Extracellular Proteases in the Coastal Sediments of King George Island, Antarctica

Zhou

Wang

et al. 2013

PLoS ONE

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Protease-producing bacteria play a vital role in degrading sedimentary organic nitrogen. However, the diversity of these bacteria and their extracellular proteases in most regions remain unknown. In this paper, the diversity of the cultivable protease-producing bacteria and of bacterial extracellular proteases in the sediments of Maxwell Bay, King George Island, Antarctica was investigated. The cultivable protease-producing bacteria reached 105 cells/g in all 8 sediment samples. The cultivated protease-producing bacteria were mainly affiliated with the phyla Actinobacteria, Firmicutes, Bacteroidetes, and Proteobacteria, and the predominant genera were Bacillus (22.9%), Flavobacterium (21.0%) and Lacinutrix (16.2%). Among these strains, Pseudoalteromonas and Flavobacteria showed relatively high protease production. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria were serine proteases or metalloproteases. These results begin to address the diversity of protease-producing bacteria and bacterial extracellular proteases in the sediments of the Antarctic Sea.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of Both the Cultivable Protease-Producing Bacteria and Bacterial Extracellular Proteases in the Coastal Sediments of King George Island, Antarctica

Zhou

Wang

et al. 2013

PLoS ONE

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“…Enzyme analysis was qualitatively conducted using an agar (Himedia-RM026, India) medium, with a suitable substrate for each enzyme. A protease was determined on skim milk powder (Himedia-GRM1254, India) agar media as suggested by Vazquez & Mac Cormack,(2002), lipase activity was determined on tributyrin agar (Himedia-M157, FD081, India) media as described by Veerapagu et al, (2013), gelatinase activity was determined on gelatinase agar (Merck-M9512jh70, Germany) as described by Sharma et al, (2015) and colloidal chitin agar was used to determine the chitinase activity as suggested by Jha et al, (2016). The cultures' enzyme activity was reported as mentioned by (Tennalli, 2012).…”

Section: Qualitative Extra-cellular Enzyme Analysismentioning

confidence: 99%

The characterization of chitinolytic soil bacterial isolates for their antagonistic activity against root-knot nematode Meloidogyne incognita: An effort towards developing 'green' nematicidal agents.

Pawar¹,

Doshi²,

Dandekar³

et al. 2022

Preprint

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Root-knot nematodes (Meloidogyne spp.) are one of the most infective pests for a wide range of cultivated plants. The study was aimed to isolate chitinolytic soil bacteria, characterizing their properties, and to determine their in vitro antagonism against root-knot nematode eggs and juveniles. Out of the Ninety-five isolated strains, twenty-five gram-positive and non-hemolytic strains were selected and screened for production of extra-cellular enzymes, such as chitinase, protease, lipase, gelatinase, etc., as well as for biofilm formation, anti-fungal activity, and in vitro antagonism against root-knot nematodes. Eleven strains demonstrated broad anti-fungal activity against common plant pathogenic fungi, like Fusarium oxysporum and Alternaria alternata. Among the twenty-five strains, ten strains exhibited statistically significant (P˂0.05) second stage juvenile (J2s) percent mortality (>60 %) and inhibited egg hatching. The in-vitro activity of these ten strains was found to be correlated with their biofilm formation, enzyme production, and anti-fungal activity. Further, out of these ten strains, nine strains were assigned to the Bacilli group (99-100% similarity), and one was identified as Paenibacillus xylanilyticus (96 % similarity) after sequencing the gyrB gene. Overall, the present in vitro study suggested that the chitinolytic bacterial strains showing biofilm formation, enzyme production, and anti-fungal activity demonstrate an important trait to exhibit antagonism against root-knot nematodes.

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“…Several authors [176,177] suggested the dependence of isolation temperature on the proteases and, more generally, on cold-enzyme (i.e., cellulase and pectate lyase) characteristics and functions, without any dependence on the taxonomic affiliation of producer nor the chemical nature of enzyme. These findings therefore highlight the importance of the screening strategies chosen for detecting psychrotolerant bacteria able to produce strong or weak cold adapted enzymes.…”

Section: Cold-enzyme Producers From Abiotic Matricesmentioning

confidence: 99%

The Variety and Inscrutability of Polar Environments as a Resource of Biotechnologically Relevant Molecules

Rizzo

Giudice

2020

Microorganisms

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The application of an ever-increasing number of methodological approaches and tools is positively contributing to the development and yield of bioprospecting procedures. In this context, cold-adapted bacteria from polar environments are becoming more and more intriguing as valuable sources of novel biomolecules, with peculiar properties to be exploited in a number of biotechnological fields. This review aims at highlighting the biotechnological potentialities of bacteria from Arctic and Antarctic habitats, both biotic and abiotic. In addition to cold-enzymes, which have been intensively analysed, relevance is given to recent advances in the search for less investigated biomolecules, such as biosurfactants, exopolysaccharides and antibiotics.

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Effect of isolation temperature on the characteristics of extracellular proteases produced by Antarctic bacteria

Cited by 9 publications

References 33 publications

Diversity of Both the Cultivable Protease-Producing Bacteria and Bacterial Extracellular Proteases in the Coastal Sediments of King George Island, Antarctica

Diversity of Both the Cultivable Protease-Producing Bacteria and Bacterial Extracellular Proteases in the Coastal Sediments of King George Island, Antarctica

The characterization of chitinolytic soil bacterial isolates for their antagonistic activity against root-knot nematode Meloidogyne incognita: An effort towards developing 'green' nematicidal agents.

The Variety and Inscrutability of Polar Environments as a Resource of Biotechnologically Relevant Molecules

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