Effect of isoprenaline on the secretion of sialoproteins from rat salivary glands

Byrt, Pauline N.; Glanvill, S.

doi:10.1016/0304-4165(67)90296-6

Cited by 33 publications

(4 citation statements)

References 10 publications

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“…In addition, acinar cells of the submandibular gland were degranulated, but depletion was less pronounced than in the parotid gland. Similarly, studies on salivary gland function with isoproterenol indicated a relatively higher increase in protein concentration in parotid than in submandibular saliva (Byrt & Glanvill 1967;Abe & Dawes 1978). Granular convoluted tubule cells remained unaffected.…”

Section: Discussionmentioning

confidence: 89%

Degranulation of Rat Salivary Glands Following Treatment With Receptor‐selective Agonists

Peter

Vanwaarde

Vissink

et al. 1995

Clin Exp Pharma Physio

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1. The aim of this study was to find a drug that induces an almost complete degranulation of secretory cells in rat parotid and submandibular glands. 2. Phenylephrine (alpha-adrenergic), isoproterenol (beta-adrenergic) and mecholine (muscarinic cholinergic) were tested. Time and degree of maximal depletion of acinar and granular convoluted tubule cells were determined morphologically. 3. Following phenylephrine-injection (5 mg/kg or 10.2 mg/kg, i.p.), no effect on the acinar granulation level was observed in either of the glands, while about 50-60% granular convoluted tubules were degranulated for at least 120-180 min post-injection. 4. With isoproterenol (5, 10, 40, 70 or 100 mg/kg, i.p.), degranulation of 100% of the acinar cells in the parotid and 80% of the acinar cells in the submandibular gland was observed 90 min post-injection. Granular convoluted tubule cells did not respond to this beta-adrenergic drug. 5. Mecholine (3.75 or 7.5 mg/kg, i.p.) induced mainly degranulation of granular convoluted tubule cells (about 50% after 120 min). Numbers of granulated acinar cells decreased only slightly in both glands (about 10%, 90-120 min). 6. From this study it appears that with a relatively low dosage (5 mg/kg, i.p.) of isoproterenol, a high level of degranulation can be induced in acinar cells of rat parotid and submandibular glands without toxic side effects. Concerning granular convoluted tubules, only moderate degranulation was observed with phenylephrine and mecholine, respectively.

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Section: Discussionmentioning

confidence: 89%

Degranulation of Rat Salivary Glands Following Treatment With Receptor‐selective Agonists

Peter

Vanwaarde

Vissink

et al. 1995

Clin Exp Pharma Physio

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“…Adrenaline is a stimulator of secretion in this tissue (Byrt & Glanvill, 1967;Pritchard, 1972) and therefore it was decided to see whether it could modify the interactions between glucose and ketone bodies. When adrenaline was added to the incubation medium at concentrations (10,ug/ml) reported to 1975 stimulate secretion of amylase in vitro (Ueha et al, 1971), a 25% increase of glucose removal compared with the control value was observed in the absence or presence of acetoacetate (Table 1).…”

Section: Effects Of Adrenaline On Glucose Metabolismmentioning

confidence: 99%

Metabolic interactions of glucose, acetoacetate and adrenaline in rat submaxillary gland in vitro

Thompson¹,

Williamson²

1975

Biochemical Journal

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1. The metabolic interactions between glucose, acetoacetate and adrenaline were studied in submaxillary-gland slices. 2. Acetoacetate (2.5 mM) inhibited glucose removal by 22% and entry of glucose carbon into the tricarboxylic acid cycle by 54%. 3. Acetoacetate caused an increase in (glucose 6-phosphate) together with an increase in (citrate), a finding that suggests that the phosphofructokinase step might be inhibited by the elevated (citrate). Support for this suggestion was obtained in experiments in which fluoracetate was used to elevate (citrate). 4. A further site of action of acetoacetate at the pyruvate dehydrogenase step was suggested by an increase in the lactate+pyruvate pool, and the finding that pyruvate removal and (3-14C)pyruvate oxidation were inhibited by acetoacetate. 5. Adrenaline, a stimulator of secretion by this tissue, increased glucose removal by 25%. Adrenaline increased glucose removal to the same extent when acetoacetate was also present in the incubation medium. In both cases the increase was accompanied by a fall in (glucose 6-phosphate). 6. Adrenaline also overcame the inhibition of pyruvate removal caused by acetoacetate. 7. The tissue (ATP) decreased by about 50% on addition of adrenaline, and a similar fall was observed in vivo after adrenergic stimulation by isoproterenol. 8. Omission of Ca-2+ from the medium prevented the fall in (glucose 6-phosphate) and (ATP) caused by adrenaline, although adrenaline was still able to stimulate glucose removal. The inhibitory effect of acetoacetate on gluocse removal was reversed by adrenaline, but there was no stimulation above the control rates. Inhibition of pyruvate removal by acetoacetate was not overcome by adrenaline in the absence of Ca-2+. 9. Dibutyryl cyclic AMP had no effect on glucose removal or on (ATP). 10. Possible mechanisms by which adrenaline can bring about its metabolic effects are discussed.

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“…Certain adrenergic antagonists (phentolamine, reserpine) appear to potentiate or mimic these effects of isoproterenol (Mandel & Katz, 1968;Martinez et al, 1975a, b). Submandibular glands produce sialic acid-rich glycoproteins which bind Ca2f readily (Leung & Draus, 1962;Rolla & Jonsen, 1967), and the sialic acid content of submandibular glands is increased following isoproterenol administration (Shackleford & Klapper, 1962;Byrt & Glanvill, 1967;Curbelo et al, 1968). If calcium is responsible for intra-granule aggregation of secretory material, one would expect the saliva from male rats to possess higher Ca2+ concentrations than females.…”

Section: Discussionmentioning

confidence: 99%

Morphology and cytochemistry of acinar secretory granules in normal and isoproterenol‐treated rat submandibular glands

Simson

Dom

Sannes

et al. 1978

Journal of Microscopy

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Several fixation procedures have been utilized in a fine-structural study of rat submandibular glands with the aim of correlating the morphology of granule substructure with its composition. Procedures included fixation with aldehyde in a variety of buffers, with or without fixation additives such as tannic acid or calcium chloride. Both immersion and perfusion fixation studies were performed. Osmium tetroxide postfixed tissue was compared with non-postfixed tissue. Thin sections were stained for carbohydrate-containing constituents by either periodic acid-thiocarbohydrazide-silver proteinate or tannic acid-ferric chloride sequences. The morphology and cytochemistry of acinar secretory granules were highly dependent upon the fixation procedure utilized. In postfixed tissue, fine filaments and vesicles were the major granule constituents. Filaments often aggregated into fibrils in fixatives containing phosphate buffer or calcium, whereas vesicles were prominent with fixatives containing collidine buffer. Tannic acid, as a fixation additive, imparted enhanced density to the peripheral rim of aggregated filaments. If post fixation was eliminated, an amorphous meshlike material was the major granule component. This stained readily with methods for carbohydrate whereas the granules of post-osmicated tissue did not. Following chronic isoproterenol treatment the degree of filament aggregation in postfixed tissue was augmented, and this corresponded to an increase in amount of material stainable for carbohydrate in non-post-osmicated tissue.

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Effect of isoprenaline on the secretion of sialoproteins from rat salivary glands

Cited by 33 publications

References 10 publications

Degranulation of Rat Salivary Glands Following Treatment With Receptor‐selective Agonists

Degranulation of Rat Salivary Glands Following Treatment With Receptor‐selective Agonists

Metabolic interactions of glucose, acetoacetate and adrenaline in rat submaxillary gland in vitro

Morphology and cytochemistry of acinar secretory granules in normal and isoproterenol‐treated rat submandibular glands

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