Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts.

Hayashi, Matsuhiko; Yamaji, Yasuyoshi; Iyori, Masahiro; Kitajima, Waichi; Saruta, Takao

doi:10.1172/jci115112

Cited by 31 publications

(9 citation statements)

References 24 publications

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“…In this study, the most likely explanation of our results is inhibition by FURO or DIDS of HCO3-/C1-exchange coupled to Na+-HCO3 -symport (see above). The appearance of active C1-reabsorption in the presence of TERB is consistent with the known stimulation (or permission) of HCOs-/C1--exchange by TERB (Harada et al, 1991;Hayashi et al, 1991;Dunk et al, 1989;Schuster and Stokes, 1987;Schuster, 1986). Both transport processes have recently been shown (Nord et al, 1988;Lubman and Crandall, 1991) to be present in alveolar epithelial cells.…”

Section: Ion Flux Studiessupporting

confidence: 84%

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Kim

Suh

Lubman

et al. 1992

Journal of Tissue Culture Methods

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SUMMARY: To investigate the celt physiologic and biological properties of the alveolar epithelium, we studied rat alveolar epithelial cell monolayers grown on permeable supports in primary culture. Type II alveolar epithelial cells were disaggregated using elastase, and partially purified on a discontinuous metrizamide gradient. These isolated cells were plated onto tissue culture-treated Nuclepore membrane filters at 1.5 )< 106 cells/cm 2 and maintained in a humidified incubator (5% CO2 in air, 37 ° C). After 2 days in culture, the bathing media on both sides of the cell monolayers were changed to fresh culture medium, thus removing nonadherent cells (mostly lcukocytes). These monolayers exhibit a high transmonolayer resistance (>2000 ~-cm 2) and actively transport ions. Radionuclide flux studies indicate that Na + is the predominant ionic species absorbed actively under baseline conditions, accounting for about 80% of the total active ion transport. CI-seems to be passively transported across the epithelium. However, when the epithelium is exposed to a beta-agonist (terbutaline), active absorption of Na + is increased ar, d active absorption of C1-occurs. Ahhough it is clear that both active Na + and CI-transport are dependent on Na+/K+-ATPase activity, and that Na + enters cells predominantly through channels, the specific mechanisms by which CI-enters and exits the alveolar epithelial cells remain unclear. The stimulated reabsorption of Na + and CImay be important in helping to remove excess fluid from alveolar air spaces in the lung.

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Section: Ion Flux Studiessupporting

confidence: 84%

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Kim

Suh

Lubman

et al. 1992

Journal of Tissue Culture Methods

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“…(Scale bar, 1 μm. ) ther, isoproterenol by increasing cAMP in the β-intercalated cell stimulated apical Cl:HCO 3 exchange (26). The complexity of regulation of acid/base transport is highlighted by these short-term and the hensin-mediated long-term changes described in this paper.…”

Section: Discussionmentioning

confidence: 67%

Deletion of hensin/DMBT1 blocks conversion of β- to α-intercalated cells and induces distal renal tubular acidosis

Gao

Eladari

Leviel

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Acid–base transport in the renal collecting tubule is mediated by two canonical cell types: the β-intercalated cell secretes HCO 3 by an apical Cl:HCO 3 named pendrin and a basolateral vacuolar (V)-ATPase. Acid secretion is mediated by the α-intercalated cell, which has an apical V-ATPase and a basolateral Cl:HCO 3 exchanger (kAE1). We previously suggested that the β-cell converts to the α-cell in response to acid feeding, a process that depended on the secretion and deposition of an extracellular matrix protein termed hensin (DMBT1). Here, we show that deletion of hensin from intercalated cells results in the absence of typical α-intercalated cells and the consequent development of complete distal renal tubular acidosis (dRTA). Essentially all of the intercalated cells in the cortex of the mutant mice are canonical β-type cells, with apical pendrin and basolateral or diffuse/bipolar V-ATPase. In the medulla, however, a previously undescribed cell type has been uncovered, which resembles the cortical β-intercalated cell in ultrastructure, but does not express pendrin. Polymerization and deposition of hensin (in response to acidosis) requires the activation of β1 integrin, and deletion of this gene from the intercalated cell caused a phenotype that was identical to the deletion of hensin itself, supporting its critical role in hensin function. Because previous studies suggested that the conversion of β- to α-intercalated cells is a manifestation of terminal differentiation, the present results demonstrate that this differentiation proceeds from HCO 3 secreting to acid secreting phenotypes, a process that requires deposition of hensin in the ECM.

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“…Fura-2 was excited alternately with light at 340 and 380 nm, and the intensity of the emitted light was measured with a photomultiplier tube through a 500-nm bandpass filter. Fluorescence measurement was performed with an Olympus OSP system, as described previously (Olympus Optical, Tokyo, Japan) ( 14). The signal ratio at two excitation wavelengths was determined after subtracting autofluorescence.…”

Section: Introductionmentioning

confidence: 99%

Pressure promotes DNA synthesis in rat cultured vascular smooth muscle cells.

Hishikawa¹,

Nakaki²,

Marumo³

et al. 1994

J. Clin. Invest.

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117

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High blood pressure is one of the major risk factors for atherosclerosis. In this study, we examined the effects of pressure on cell proliferation and DNA synthesis in cultured rat vascular smooth muscle cells. Pressure without shear stress and stretch promotes cell proliferation and DNA synthesis in a pressuredependent manner. Pressure-induced DNA synthesis was inhibited significantly by the phospholipase C (PLC) inhibitor 2-nitro4-carboxyphenyl-N,N-diphenylcarbamate, the protein kinase C inhibitor H-7, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, staurosporine, and the tyrosine kinase inhibitor (l3,4,5-trihydroxyphenyljmethylene) propanedinitrile. Toclarify whether activation of PLC and calcium mobilization are involved in pressure-induced DNA synthesis, production of 1,4,5-inositol trisphosphate (IP3) and intracellular Ca2" was measured. Pure pressure increased IP3 and intracellular Ca2"

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Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts.

Cited by 31 publications

References 24 publications

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Deletion of hensin/DMBT1 blocks conversion of β- to α-intercalated cells and induces distal renal tubular acidosis

Pressure promotes DNA synthesis in rat cultured vascular smooth muscle cells.

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