Effect of jasmonates and exogenous polysaccharides on production of alkannin pigments in suspension cultures of Alkanna tinctoria

Urbanek, H.; Bergier, K.; Saniewski, M.; Patykowski, Jacek

doi:10.1007/bf00232468

Cited by 24 publications

(5 citation statements)

References 7 publications

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“…( Beerhues and Berger, 1995 ). A marked increase in the alkannin pigment content in cells and medium of suspension cultures of Alkanna tinctoria after treatment with MJ was observed ( Urbanek et al, 1996 ). The addition of 0.5 μM MJ provoked a twofold to threefold increase in anthocyanin production over that of the control in cell cultures of Vaccinium pahalae ( Fang et al, 1999 ).…”

Section: Elicitation Of Secondary Metabolites Using Jasmonatesmentioning

confidence: 99%

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

et al. 2022

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Secondary metabolites are incontestably key specialized molecules with proven health-promoting effects on human beings. Naturally synthesized secondary metabolites are considered an important source of pharmaceuticals, food additives, cosmetics, flavors, etc., Therefore, enhancing the biosynthesis of these relevant metabolites by maintaining natural authenticity is getting more attention. The application of exogenous jasmonates (JAs) is well recognized for its ability to trigger plant growth and development. JAs have a large spectrum of action that covers seed germination, hypocotyl growth regulation, root elongation, petal expansion, and apical hook growth. This hormone is considered as one of the key regulators of the plant’s growth and development when the plant is under biotic or abiotic stress. The JAs regulate signal transduction through cross-talking with other genes in plants and thereby deploy an appropriate metabolism in the normal or stressed conditions. It has also been found to be an effective chemical elicitor for the synthesis of naturally occurring secondary metabolites. This review discusses the significance of JAs in the growth and development of plants and the successful outcomes of jasmonate-driven elicitation of secondary metabolites including flavonoids, anthraquinones, anthocyanin, xanthonoid, and more from various plant species. However, as the enhancement of these metabolites is essentially measured via in vitro cell culture or foliar spray, the large-scale production is significantly limited. Recent advancements in the plant cell culture technology lay the possibilities for the large-scale manufacturing of plant-derived secondary metabolites. With the insights about the genetic background of the metabolite biosynthetic pathway, synthetic biology also appears to be a potential avenue for accelerating their production. This review, therefore, also discussed the potential manoeuvres that can be deployed to synthesis plant secondary metabolites at the large-scale using plant cell, tissue, and organ cultures.

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Section: Elicitation Of Secondary Metabolites Using Jasmonatesmentioning

confidence: 99%

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

et al. 2022

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show abstract

“…The type of plant growth regulators and explant may be fundamental factors for production of A/S derivates (Urbanek et al 1996) which optimized suspension cultures of A. tinctoria for efficient production of alkannin. B5 medium supplemented with 2 mg/l 2-4, D, 0.5 mg/l BAP and 7 g/l agar was reported to be suitable for callus formation of A. tinctoria using root.…”

Section: Resultsmentioning

confidence: 99%

“…Commercial production of A/S and its derivatives were first produced by callus culture of Lithospermum erythrorhizon (Tabata et al 1974). It is also reported that A/S and it derivatives were produced using tissue culture techniques for different species, e.g., callus culture of Alkanna tinctoria (Urbanek et al 1996), root and suspension culture of Lithospermum canescens (Tatsumi et al 2016), suspension culture of Echium italicum L. (Zare et al 2010), callus culture and suspension culture of Arnebia hispidissima (Singh and Sharma 2014), callus culture of Onosma bulbotrichom (Bagheri et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Callus induction and regeneration of Alkanna orientalis var. Orientalis and A. sieheana

Yaman

Uranbey

Ahmed³

et al. 2019

Bangladesh J. Bot.

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Callus induction and proliferation of Alkanna orientalis var. orientalis and Alkanna sieheana containing valuable alkannin/shikonin (A/S) derivates were investigated using leaf base and stem segment explants. Stem segments and cotyledonary leaf base of both species were cultured on Murashige and Skoog medium fortified with different concentrations of BAP, Kn, NAA, IAA and IBA for callus induction and shoot regeneration. High frequency reproducible, prolific and compact calli formation was obtained from the stem segments of both species in all media tested. The frequency variations of callus induction and shoot regeneration were discussed in terms of different species, plant growth regulators and explant resources. A. orientalis and A. sieheana may be considered to be alternative plants for the A/S production in vitro.

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“…Various studies have reported increased production of alkanin/shikonin and their derivatives (A/S) in cell cultures of Boragenaceous plants (e.g., Lithospermum erythrorhizon, Alkanna tinctoria, and Arnebia euchroma) after applying exogenous jasmonate (Gaisser and Heide 1996;Urbanek et al 1996;Bychkova et al 1993). Alkanin/shikonin are naphtoquinone compounds with a broad spectrum of biological activities, such as wound healing, anti-inflammatory, and anticancer (Kheiri et al 2017;Kourounakis et al 2002;Andújar et al 2013).…”

Section: Mechanisms By Which Amf Symbiosis Promotes Secondary Metabol...mentioning

confidence: 99%

Arbuscular mycorrhizal fungi and production of secondary metabolites in medicinal plants

et al. 2022

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Medicinal plants are an important source of therapeutic compounds used in the treatment of many diseases since ancient times. Interestingly, they form associations with numerous microorganisms developing as endophytes or symbionts in different parts of the plants. Within the soil, arbuscular mycorrhizal fungi (AMF) are the most prevalent symbiotic microorganisms forming associations with more than 70% of vascular plants. In the last decade, a number of studies have reported the positive effects of AMF on improving the production and accumulation of important active compounds in medicinal plants.In this work, we reviewed the literature on the effects of AMF on the production of secondary metabolites in medicinal plants. The major findings are as follows: AMF impact the production of secondary metabolites either directly by increasing plant biomass or indirectly by stimulating secondary metabolite biosynthetic pathways. The magnitude of the impact differs depending on the plant genotype, the AMF strain, and the environmental context (e.g., light, time of harvesting). Different methods of cultivation are used for the production of secondary metabolites by medicinal plants (e.g., greenhouse, aeroponics, hydroponics, in vitro and hairy root cultures) which also are compatible with AMF. In conclusion, the inoculation of medicinal plants with AMF is a real avenue for increasing the quantity and quality of secondary metabolites of pharmacological, medical, and cosmetic interest.

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Effect of jasmonates and exogenous polysaccharides on production of alkannin pigments in suspension cultures of Alkanna tinctoria

Cited by 24 publications

References 7 publications

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

Callus induction and regeneration of Alkanna orientalis var. Orientalis and A. sieheana

Arbuscular mycorrhizal fungi and production of secondary metabolites in medicinal plants

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