Effect of jasmonic acid on cold-storage of Taraxacum pieninicum encapsulated shoot tips

Kamińska, M.; Tretyn, Andrzej; Trejgell, Alina

doi:10.1007/s11240-018-1481-y

Cited by 16 publications

(3 citation statements)

References 49 publications

(52 reference statements)

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“…Хотя для Taraxacum pieninicum использование абсцизовой кислоты было оптимальным вариантом. Материал хранили до 9 месяцев [51]. Экспланты Vitis heyneana хранили до 12 месяцев с последующей 48%-ной регенерацией [52].…”

Section: результаты и обсуждениеunclassified

Optimization of the medium-term storage protocol for the endangered Malus niedzwetzkyana

Nurtaza¹,

Dyussembekova²,

Kakimzhanova³

2022

bulbioenukz

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The conservation of biodiversity requires efficient ex situ methods for the storage of plant genetic material outside the natural habitat. This approach allows for the preservation of valuable, endemic, and endangered plant species for a long time. One widely used method is medium-term storage in in vitro culture. In our work, we describe the results of medium-term storage of microclonally propagated shoots of an endangered and endemic species of the Malus niedzwetzkyana. In vitro preservation was realized with the help of osmotic agents in the nutrient medium. For this, there were studied the cultivation conditions such as temperature (4°C and 24°C) and photoperiod (16/8 and growing in the dark), and the composition of nutrient media to slow growth (sucrose, mannitol, PVP, abscisic acid). The research results showed that the optimal condition for medium-term storage of shoots is the QL nutrient medium with the addition of 3% or 6% sucrose under standard conditions (24°C, photoperiod 16/8). The optimized protocol made it possible to preserve Malus niedzwetzkyana explants for 2 months without recultivation. Moreover, the stage of explant recovery was fast, during only one passage.

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Section: результаты и обсуждениеunclassified

Optimization of the medium-term storage protocol for the endangered Malus niedzwetzkyana

Nurtaza¹,

Dyussembekova²,

Kakimzhanova³

2022

bulbioenukz

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“…For these reasons optimization of the slow growth conditions is highly desirable to maximize time between passages. Lowering the temperature of T. pieninicum shoot tips culture allowed its storage for several months (Kamińska et al 2018a), although increased survival of the explants was noted after encapsulation (Kamińska et al 2018b). The possibility of extending the storage time of encapsulated explants by using abscisic acid as a growth retardant was evaluated in this paper.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions

Kamińska

Kęsy

Trejgell

2020

Plant Cell Tiss Organ Cult

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Taraxacum pieninicum Pawł. is listed as critically endangered species, for which currently applied protection methods are insufficient. The aim of this study was to investigate the possibility of T. pieninicum storage in the form of synthetic seeds under slow-growth conditions in combination with ABA treatment, as one of the ex situ protection methods of this species. The obtained results indicated that darkness was much more favorable condition for synseed storage and did not generate additional stress during cold exposure in contrast to the light conditions. The preculture of shoot tips on the medium supplemented with ABA led to a decrease in the shoots proliferation rate and inhibition of their growth. ABA clearly inhibited growth of the encapsulated shoot tips also during cold storage. Biochemical parameters showed that ABA effectively reduced the negative effect of the cold stress, what was found on the basis of analyzes of H2O2 and TBARS levels in the stored material. Moreover, synseeds stored under light conditions and treated with ABA exhibited decreased level of endogenous jasmonic acid what indicated interaction between those two phytohormones at a low temperature. The study also demonstrated that in vitro culture, cold storage and ABA treatment had no effect on the flowering process of this species after acclimatization to ex vitro conditions.

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“…The relationship between dynamic changes in antioxidant enzyme activity and seed aging has been documented [21], and such changes can be considered one of the potential indicators of seed aging [22]. The accumulation of proline (PRO), a compatible osmolyte, can also eliminate ROS and improve a plant's ability to survive under stress [23][24][25]. In addition, the accumulation of malondialdehyde (MDA), one of the leading products of lipid peroxidation of the seed cell membrane, disrupts the antioxidant system and leads to a decrease in seed viability [26,27], making the content an evaluation indicator for the extent of cell membrane damage.…”

Section: Introductionmentioning

confidence: 99%

Potential Biochemical Markers Affecting Aging and “the Compensatory Effects” of Canola (Brassica napus L.) Seeds Stored in Deep Underground

Wang

Tan

et al. 2023

Agriculture

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Understanding the impact of deep underground environment on seed storage is an essential prerequisite for realizing the idea of “deep underground agriculture”. To explain the changes in seed germination performance after deep underground storage, we examined the biochemical properties of envelope-packed canola seeds stored for three different durations (66, 90, and 227 days) in four different depths (0, 240, 690, and 1410 m) of a gold mine. Results showed that deep underground storage duration was the leading cause of biochemical properties changes of canola seed, while storage depth exacerbated such changes. Deep underground environment significantly suppressed seed superoxide dismutase (SOD) and soluble sugar (SS), which could be the main reason for the accelerated loss of seed vigor. The appearance of the “compensatory effect” was mainly attributed to the increase in seed growth hormone and the decrease in abscisic acid (ABA) content. The most significant enhancement in growth hormone content was observed in seeds stored at a depth of 1410 m for 66 days, where indoleacetic acid (IAA), gibberellin (GA), and cytokinin (CTK) increased by 63.37%, 21.77%, and 79.36%, respectively. In this study, short-term deep underground storage could enhance seedling growth, but the recommended storage duration for canola seeds should not be longer than 90 days.

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Effect of jasmonic acid on cold-storage of Taraxacum pieninicum encapsulated shoot tips

Cited by 16 publications

References 49 publications

Optimization of the medium-term storage protocol for the endangered Malus niedzwetzkyana

Optimization of the medium-term storage protocol for the endangered Malus niedzwetzkyana

Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions

Potential Biochemical Markers Affecting Aging and “the Compensatory Effects” of Canola (Brassica napus L.) Seeds Stored in Deep Underground

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