The pattern in which work is structured plays a significant role in deciding how motivated, satisfied and committed an employee will be. The workers at the polytechnic are not left out of this continuous and repetitive approach in carrying out their daily routines. The study was centred on examining the relationship between job satisfaction and organizational citizenship behaviour, with a view to proffering strategies to Nigerian polytechnics on the acceptability and adoptability of job satisfaction initiative. The specific objectives are to examine the relationships between employees’ skill variety and employees’ altruism, and employees’ commitment and employees’ conscientiousness. The study was anchored on Job Characteristics Theory. The study adopted the descriptive survey research design. The population comprises 120 academic staff of the School of Business Studies in Federal Polytechnic, Oko. Data were collected through the use of a five-point likert style questionnaire. With the aid of IBM SPSS version 25, Spearman correlation was employed to test the hypotheses, while One sample test model was applied to determine the level of significance between the independent and dependent variables. From the test of hypotheses, the first finding of the study revealed that skill variety had a significant positive relationship with employees’ altruism (t-value of 66.715 and p-value = 0.000** at 0.05 level of significance and low positive value of r = 0.338), and the second test revealed that employee commitment had a significant positive relationship with employees’ conscientiousness (t-value of 65.591 and p-value of 0.000** at 0.05 level of significance and a moderate positive correlation value of r = 0.638) of the polytechnic. The study recommended that the management of the polytechnic should invest in training programmes for their employees to improve their skill variety on their job for sustainable organizational citizenship behavior, and reward employees who possess the act of conscientiousness in the workplace so as to increase their commitment to the organizations.