Effect of Jujube Honey on Candida albicans Growth and Biofilm Formation

Ansari, Mohammad Javed; Al-Ghamdi, Ahmad; Usmani, Salma; Al-Waili, Noori S.; Sharma, Deepak; Adgaba, Nuru; Alattal, Yehya

doi:10.1016/j.arcmed.2013.06.003

Cited by 79 publications

(43 citation statements)

References 46 publications

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“…[30] also reported that the effectiveness of Manuka honey against biofilms formed by S. aureus and P. aeruginosa were less than 100%. Nevertheless, based on this study and others, it is important to note that Manuka honey has an appreciable antibacterial activity against biofilm formed by bacterial organisms [31]. While in this study we demonstrated the ability of Manuka honey to inhibit biofilm formation of C. difficile , Maddock et al .…”

Section: Resultssupporting

confidence: 51%

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Biofilm formation of Clostridium difficile and susceptibility to Manuka Honey

Hammond

Donkor

Brown

2014

BMC Complement Altern Med

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BackgroundBiofilm bacteria are relatively more resistant to antibiotics. The escalating trend of antibiotic resistance higlights the need for evaluating alternative potential therapeutic agents with antibacterial properties. The use of honey for treating microbial infections dates back to ancient times, though antimicrobial properties of Manuka honey was discovered recently. The aim of this study was to demonstrate biofilm formation of specific Clostridium difficile strains and evaluate susceptibility of the biofilm to Manuka honey.MethodsThree C. difficile strains were used in the study including the ATCC 9689 strain, a ribotype 027 strain and a ribotype 106 strain. Each test strain was grown in sterile microtitre plates and incubated at 37°C for 24 and 48 hours in an anaerobic cabinet to allow formation of adherent growth (biofilm) on the walls of the wells. The effect of Manuka honey on the biofilms formed was investigated at varying concentrations of 1-50% (w/v) of Manuka honey.ResultsThe three C. difficile strains tested formed biofilms after 24 hours with the ribotype 027 strain producing the most extensive growth. There was no significant difference (p > 0.05) found between the amount of biofilms formed after 24 and 48 hours of incubation for each of the three C. difficile strains. A dose–response relationship between concentration of Manuka honey and biofilm formation was observed for all the test strains, and the optimum Manuka honey activity occurred at 40-50% (v/v).ConclusionManuka honey has antibacterial properties capable of inhibiting in vitro biofilm formed by C. difficile.

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Section: Resultssupporting

confidence: 51%

“…Recently, Ansari et al . [31] also reported that Jujube honey can disrupt pre-formed biofilms of Candida albicans . Antimicrobial effect of Manuka honey is due to a property referred to as Unique Manuka Factor that is absent in other types of honey [32].…”

Section: Resultsmentioning

confidence: 99%

Biofilm formation of Clostridium difficile and susceptibility to Manuka Honey

Hammond

Donkor

Brown

2014

BMC Complement Altern Med

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“…6). According to previous literature the absorption peaks for sugars in the EPS ranged from the mid-infra red region of 800-1128 cm −1 (Ansari et al 2013). These peaks clearly indicated the presence of ␤-glucans and mannans in C. albicans exopolysaccharide and there was a decrease in the intensity of the peak height in the US treated biofilms when compared to the control.…”

Section: Ft-ir Analysis Of C Albicans Biofilmsmentioning

confidence: 98%

Usnic acid inhibits biofilm formation and virulent morphological traits of Candida albicans

Nithyanand

Shafreen

Muthamil

et al. 2015

Microbiological Research

104

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Biofilm formation and the yeast to hyphal switch are considered to be important virulence factors of Candida albicans. The present study reports about the potential of usnic acid, a lichen secondary metabolite inhibiting these virulent factors. Usnic acid, at its biofilm inhibitory concentration (BIC) largely reduced the viability of the metabolically active cells in matured C. albicans biofilms, exhibited significant biofilm inhibition (65%) and prevented the property of adhesion. Light microscopic images revealed that usnic acid effectively inhibited the yeast to hyphal switch and confocal microscopy showed that usnic acid greatly reduced the thickness of matured biofilms. Furthermore, usnic acid was able to reduce various sugars present in the exopolysaccharide layer (EPS) which was also confirmed by FT-IR analysis. Taken together, the present study showcases usnic acid as a potent anti-virulent compound against C. albicans and opens up a new avenue for bioprospecting lichen secondary metabolites as anti-virulent compounds.

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“…It has also proven useful for quantitative biofilm analysis, especially to confirm findings obtained by quantitative (viable count, CV staining) or other imaging (light microscopy, SEM) techniques (Ansari et al, 2013;Chatterjee et al, 2014;Li et al, 2015;Salunke et al, 2014). Among the characteristics of the sample surface examined, height and roughness analyses from AFM images allow quantification of biofilm biomass in terms of thickness and EPS amount, respectively (Ansari et al, 2013;Chatterjee et al, 2014;Danin et al, 2015;Li et al, 2015;Mangalappalli-Illathu et al, 2008;Nandakumar et al, 2004;Qin et al, 2009;Sharma et al, 2010).…”

Section: ;mentioning

confidence: 99%

“…First used by Bremer et al to visualize biofilms (Bremer et al, 1992), AFM has been mainly applied to gain valuable insights in biofilm structure and mechanisms underlying adhesion, as well as single-and multi-strain biofilm formation (Figure 12) (Boyd et al, 2014;Cabral et al, 2014;Lim et al, 2011;Ovchinnikova et al, 2013;Potthoff et al, 2015). It has also proven useful for quantitative biofilm analysis, especially to confirm findings obtained by quantitative (viable count, CV staining) or other imaging (light microscopy, SEM) techniques (Ansari et al, 2013;Chatterjee et al, 2014;Li et al, 2015;Salunke et al, 2014). Among the characteristics of the sample surface examined, height and roughness analyses from AFM images allow quantification of biofilm biomass in terms of thickness and EPS amount, respectively (Ansari et al, 2013;Chatterjee et al, 2014;Danin et al, 2015;Li et al, 2015;Mangalappalli-Illathu et al, 2008;Nandakumar et al, 2004;Qin et al, 2009;Sharma et al, 2010).…”

Section: ;mentioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

789

679

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Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms. ARTICLE HISTORY

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Effect of Jujube Honey on Candida albicans Growth and Biofilm Formation

Cited by 79 publications

References 46 publications

Biofilm formation of Clostridium difficile and susceptibility to Manuka Honey

Biofilm formation of Clostridium difficile and susceptibility to Manuka Honey

Usnic acid inhibits biofilm formation and virulent morphological traits of Candida albicans

Critical review on biofilm methods

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