Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth

PCE17 has been developed to prevent and treat osteoporosis in Korea. Here, we investigated the anti‐osteoporotic effects of PCE17 and its active compounds, a mixture of tectoridin, tectorigenin, and tectorigenin 7‐O‐xylosylglucoside (Tec) and hesperidin, in ovariectomized (OVX) mice. Serum levels of RANKL, TRAP, and Ntx1 were higher in OVX‐control mice than normal controls. However, these increased levels were decreased by PCE17, Tec, or hesperidin. In addition, serum levels of alkaline phosphatase, osteoprotegerin, and osteocalcin were higher in PCE17, Tec, or hesperidin‐fed mice than in OVX controls. PCE17 or hesperidin increased bone mineral density, trabecular number, and connectivity density, and decreased total porosity. Furthermore, no obvious hypercholesterolemia or liver toxicity developed in OVX‐treated mice at PCE17 dosages up to 460 mg/kg. In conclusion, PCE17 has an anti‐osteoporotic effect in OVX mice. These results suggest that PCE17 has potential use as an alternative therapeutic agent for the prevention and treatment of osteoporosis. Practical applications PCE17 has been developed to prevent osteoporosis as a health functional food. PCE17 has an anti‐osteoporotic effect through the maintenance bone homeostasis by redressing the balance between bone resorption and bone formation, and that PCE17 be considered potentially useful preventative or protective treatment for postmenopausal osteoporosis.

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“…High-resolution lCT was used to obtain 2D and 3D information on bone geometry, as previously described (Kim, Jeong, Park, & Moon, 2014).…”

Section: Microcomputed Tomography (Lct)mentioning

confidence: 99%

PCE17 and its active compounds exert an anti-osteoporotic effect through the regulation of receptor activator of nuclear factor-κB ligand in ovariectomized mice

Jeong

Kim

et al. 2018

J Food Biochem

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“…The 2D/3D information on bone geometry was provided by microcomputed tomography (mCT) according to previous study. 16…”

Section: Microcomputed Tomography (Mct)mentioning

confidence: 99%

Efficacy of proline in the treatment of menopause

Nam

Yoou

Kim

et al. 2016

Exp Biol Med (Maywood)

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The amino acids in the placenta have multiple functions; however, the therapeutic effects of proline remain poorly for relief postmenopausal symptoms. The aim of present study was to evaluate the effects of proline in the treatment of menopause using in vitro and in vivo models. We assessed the therapeutic effects and regulatory mechanisms of proline by using MCF-7 estrogen-dependent cells, MG63 osteoblast cells, and ovariectomized mice model. An in vivo study was carried out in eight-weekold sham and ovariectomized group. The ovariectomized mouse was further subdivided into two groups administered orally with 17b-estradiol or proline (10 mg/kg/day) for eight weeks. Proline significantly increased cell proliferation and Ki-67 levels in MCF-7 cells and enhanced cell proliferation, alkaline phosphatase activity, extracellular signal-regulated kinase phosphorylation, and glutamyl-prolyl-tRNA synthetase activation in MG63 cells. The estrogen receptor-b and estrogen-response elements luciferase activity were significantly increased by proline in MCF-7 and MG63 cells. In ovariectomized mice, oral administration of proline (10 mg/kg/day) for eight weeks significantly reduced body and vaginal weights. Proline also significantly increased serum estradiol and alkaline phosphatase levels, whereas serum luteinizing hormone was decreased by proline. In addition, detailed microcomputed tomography analysis showed that the proline notably enhanced bone mineral density, trabecular bone volume, and trabecular number in ovariectomized mice. Those findings implied that proline can be a promising candidate for the treatment of menopause.

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“…TNF-α, IL-6, and IL-1β secretions were measured by ELISA, as described previously (Kim et al. 2014 ). Briefly, 24-well plates were coated with 100 μL aliquots of anti-mouse TNF-α, IL-6, and IL-1β (1.0 μg/mL), respectively, and incubated overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan

Nam

Kim

et al. 2017

Pharmaceutical Biology

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Objective: To study the anti-inflammatory properties of OJ.Context: Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ.Materials and methods: Peritoneal macrophages were isolated 3–4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0.01, 0.1, and 1 mg/mL) for 1 h and stimulated with lipopolysaccharide (LPS) for different times. Nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and proinflammatory cytokine levels were determined by NO assay, Western blotting, RT-PCR and ELISA.Results: NO generation and iNOS induction were increased in the LPS-activated mouse peritoneal macrophages. However, NO generation and iNOS induction by LPS were suppressed by treatment with OJ for the first time. The IC50 value of OJ with respect to NO production was 0.09 mg/mL. OJ did not influence LPS-stimulated COX-2 induction, but did significantly decrease LPS-stimulated secretions and mRNA expressions of tumour necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Inhibition rates of TNF-α, IL-6, and IL-1β at an OJ concentration of 1 mg/mL were 77%, 88%, and 50%, respectively. OJ also suppressed the LPS-induced nuclear translocation of NF-κB. High-performance liquid chromatography showed schizandrin and gomisin A are major components of OJ.Conclusions: OJ reduces inflammatory response, and this probably explains its positive impact on the prostatitis associated inflammation.

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Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth

Cited by 9 publications

References 40 publications

PCE17 and its active compounds exert an anti-osteoporotic effect through the regulation of receptor activator of nuclear factor-κB ligand in ovariectomized mice

PCE17 and its active compounds exert an anti-osteoporotic effect through the regulation of receptor activator of nuclear factor-κB ligand in ovariectomized mice

Efficacy of proline in the treatment of menopause

Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan

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