Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves

Yoo, S.P.; Beak, Seok-Hyeon; Kang, Hyun Mi; Jung, Dooyoung; Fassah, Dilla Mareistia; Jeong, Inhyuk; Park, Seung Ju; Haque, Md. Najmul; Kim, Myunghoo; Baik, Myunggi

doi:10.5713/ab.22.0368

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…The Wako NEFA-HR2 kit (Wako Chemicals, Richmond, VA, USA) was applied for the serum NEFA analysis. The analytical methods used were validated in previous reports from our laboratory [ 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

Administration of chromium picolinate and meloxicam alleviates regrouping stress in dairy heifers

Jung,

Lee,

Kim

et al. 2024

Anim Biosci

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Objective: This research was performed to investigate effect of administering chromium (Cr) and meloxicam (MEL) on growth performance, cortisol and blood metabolite, and behaviors in young regrouped heifers.Methods: Fifty Holstein dairy heifers (body weight (BW) 198 ± 32.7 kg and 6.5 ± 0.82 months of age) were randomly assigned to non-regrouped group or four regrouped groups. Non-regrouped animals were held in the same pen throughout entire experimental period (NL: non-regrouping and administration of lactose monohydrate (LM; placebo). For regrouping groups, two or three heifers maintained in four different pens for 2 weeks were regrouped into a new pen and assigned to one of four groups: regrouping and LM administration (RL); regrouping and Cr administration (RC); regrouping and MEL administration (RM), and regrouping and Cr and MEL administration (RCM).LM (1 mg/kg BW), Cr (0.5 mg Cr picolinate/kg dry matter intake), and MEL (1 mg/kg BW) were orally administered immediately before regrouping. Blood was collected before regrouping (0 h) and at 3, 9, and 24 h and 7 and 14 d thereafter. Behaviors were recorded for 7 consecutive days after regrouping.Results: Average daily gain was lower (P < 0.05) in RL than NL heifers, but was higher (P < 0.05) in RM, RC, and RCM than RL heifers. RL heifers had higher (P < 0.05) cortisol than NL heifers on d 1 after regrouping. The cortisol concentrations in RC, RM, and RCM groups were lower (P A c c e p t e d A r t i c l e 4 < 0.05) than in RL treatment 1 d after regrouping. Displacement behavior was greater (P < 0.05) in RL group than all other groups at 2, 3, and 6 d after regrouping. Conclusion:Regrouping caused temporal stress, reduced growth performance, and increased displacement behavior in heifers. Administering Cr and MEL recovered the retarded growth rate and reduced displacement behavior, thereby alleviating regrouping stress.

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Section: Methodsmentioning

confidence: 99%

Administration of chromium picolinate and meloxicam alleviates regrouping stress in dairy heifers

Jung,

Lee,

Kim

et al. 2024

Anim Biosci

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GnRH-immunocastration: an alternative method for male animal surgical castration

Wang,

Yang,

Zeng

et al. 2023

Front. Vet. Sci.

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Castration of male animals is intended to produce high-enhance quality of animal meat, prevent unpleasant taste, reduce aggressive behavior, and manage overbreeding. Over the years, Tranditional methods of mechanical and surgical castration have been employed over the years, but they fall short of meeting animal welfare requirements due to the associated risk of infection, pain, and stress. Immunocastration, specifically Gonadotropin-releasing hormone (GnRH)-immunocastration, targeting the hypothalamic–pituitary-testis (HPT) axis, has emerged as an animal-friendly alternative to surgical castration, effectively addressing these issues. This review seeks to systematically summarize the principles, development, current applications and challenges of GnRH-immunocastration, offering insights into its role in promoting animal welfare.

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Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls

Cho,

Yoo,

Jung

et al. 2024

Front. Vet. Sci.

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This study examined the effects of surgical castration and lidocaine-plus-meloxicam treatment on growth, physiology, behaviors, and leukocyte heat shock protein 90 (HSP 90) gene expression in Hanwoo (Korean cattle) bulls. Twenty Hanwoo bulls (body weight 248.8 ± 28.5 kg, age 9.4 ± 1.04 months) were assigned to three treatments: surgical castration with lidocaine injection (5 mL in the scrotum) and oral meloxicam administration (1 mg/kg body weight; LM; n = 7); surgical castration with placebo injection (5 mL of 0.9% NaCl) and oral placebo administration (lactose, 1 mg/kg body weight; CAS; n = 7); and shame castration (SHAM; n = 6). Meloxicam and lactose were administered 3 h before castration, and lidocaine and NaCl were injected immediately before castration. Surgical castration was performed with a Newberry knife and a Henderson castration tool. Wight was measured the day before and 14 d after castration, and behavior was observed from 0.5 h to 4.5 h post-castration. Blood was collected at −1 d, 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration to measure plasma cortisol, haptoglobin, and leukocyte HSP 90 mRNA. Castration tended to decrease average daily gain (p = 0.06), but the LM treatment did not affect weight gain. Bulls in CAS showed higher cortisol concentration (p < 0.05) at 0.5 and 6 h after castration compared to SHAM, with no difference between CAS and LM. Castration increased haptoglobin concentration at 1 and 3 d after castration (p < 0.05) while LM bulls showed decreased haptoglobin concentration (p < 0.05) than CAS bulls at these times. HSP90 mRNA was increased at 6 h post-castration while LM did not reduce its expression, suggesting HSP90 may serve as an acute stress marker in castrated bulls. Castration increased (p < 0.05) frequencies of drinking, lying, walking, leg lifting, kicking, and stiff gait, while decreasing (p < 0.05) eating frequency. LM alleviated (p < 0.05) drinking, leg lifting, kicking, and stiff gait. Collectively, castration resulted in physiological changes, increased leukocyte HSP90 gene expression, and altered behaviors. These findings suggest that lidocaine-plus-meloxicam treatment partially mitigates pain and inflammation in the castrated bulls.

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Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves

Cited by 3 publications

References 31 publications

Administration of chromium picolinate and meloxicam alleviates regrouping stress in dairy heifers

Administration of chromium picolinate and meloxicam alleviates regrouping stress in dairy heifers

GnRH-immunocastration: an alternative method for male animal surgical castration

Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls

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