Effect of L-arginine and corvitin on oxidative-nitrosative stress in skin of rats exposed to excessive sodium nitrate

Khmil, D. O.; Костенко, Віталій Олександрович

doi:10.15407/fz63.06.053

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…Animals from fourth group on the background of WAS modelling received 10 mg/kg intraperitoneally corvitin, a quercetin complex with polyvinylpyrrolidine produced by ZAT NVTS "Borshchahivsʹkyy CPP", Ukraine (dose was calculated in terms of quercetin) each day [12].…”

Section: Methodsmentioning

confidence: 99%

Influence of corvitin and metformin on biochemical changes in lacrimal glands of rats during water avoidance stress modeling

Matsytska¹,

Akimov²,

Mykytenko³

2022

Oftalmol Zh

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Background. Dry eye disease is a multifactorial condition, which is characterized by impairment of tear film formation. Lacrimal gland metabolism plays a critical role in dry eye disease. Emotional stress may impair lacrimal gland function.Purpose. We aimed to study production of nitric oxide from constitutive and inducible NOsynthases, activity of arginases and oxidative stress markers in lacrimal glands of rats during modeling of water avoidance stress (WAS) and its correction by metformin and corvitin. Material and methods. We concluded our experiment on 36 adult male rats of Wistar line weighing 190-240 g. Animals were divided into 6 groups consisting of 6 animals each, namely: control group, WAS group, group of correction by metformin (200 mg/kg) and group of correction by corvitin (10 mg/kg) during WAS modeling. And two drug-control groups.Results. WAS leads to increased activity of inducible NO-synthase, superoxide dismutase, catalase and concentration of MDA by 1.59, 1.93, 1.97 and 1.28 times respectively. Metformin and corvitin decreased activity of inducible NO-synthase by 8.25 and 8.5 times respectively, concentration of MDA decreased by 1.35 and 1.26 times respectively. Activities of superoxide dismutase did not change after introduction of metformin and corvitin. Metformin decreased catalase activity by 1.47 and corvitin increased it by 1.55 times. Production of superoxide dropped during WAS by 1.59 times and was increased to level below or equal to that of control animals with introduction of metformin and corvitin. Conclusion. Increased activity of inducible NO-synthase during WAS is a possible reason of tissue damage in lacrimal glands of rats. Introduction of metformin or corvitin duringWAS is an effective means for correction of tissue damage in lacrimal glands of rats due to their ability to lower increased inducible NO-synthase activity.

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Section: Methodsmentioning

confidence: 99%

Influence of corvitin and metformin on biochemical changes in lacrimal glands of rats during water avoidance stress modeling

Matsytska¹,

Akimov²,

Mykytenko³

2022

Oftalmol Zh

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“…For local administration, S. typhi LPS was introduced once in as dose of 1 µg / kg, equally divided into four injections into the gum at the level of the second molars 7 days prior to the decapitation (acute gingivitis model). Water-soluble form of quercetin (corvitin, "Borshchahivskiy CPP", Ukraine) was administered intraperitoneally in a daily dose of 10 mg / kg recalculated for quercetin [5], and EGCG (Sigma-Aldrich, Inc., USA) was administered in a dose of 21.1 mg / kg [17] 3 times a week, starting on the 30 th day of the systemic LPS administration. The research was guided by the principles of biomedical ethics.…”

Section: Methodsmentioning

confidence: 99%

“…A particular role of ARE is determined by the fact that transcriptional nuclear factors κB (NF-κB) and activator protein 1 (AP-1) activation depends on its activity [13]. Nowadays, the ability of quercetin to influence on the activity of enzymes involved in the degradation of phospholipids (phospholipase, lipoxygenase, cyclooxygenase), and to block oxidative stress-dependent connective tissue disruption has already been proven [5]. There is evidence suggesting quercetin ability to activate the Nrf2-dependent HO-1 pathway and suppress ubiquitin-dependent proteolysis of NF-κB with an inhibitory protein IκB, which impairs the degradation of the latter under the proteasome action [3].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Quercetin and Epigallocatechin-3-Gallate as Agents to Prevent Connective Tissue Disintegration in the Periodontium of Rats Under Systemic and Local Administration of Lipopolisaccharide of Salmonella Typhi

Yelins’ka

Костенко

2019

Med. and Ecol. probl.

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The aim of the present study was to investigate the co-effect produced by water-soluble form of quercetin and epigallocatechin-3-gallate (EGCG) on biochemical markers of periodontal organic matrix depolimerization under systemic administration and local application of S. typhi lipopolisaccharide (LPS). The studies were conducted on 30 white rats of the Wistar line weighing 180-220 g, divided into 5 groups: the 1st included intact animals, the 2nd was made up of animals after the combined systemic and local LPS administration, the 3rd and 4th groups included animals, which were being given injections with water-soluble form of quercetin (10 mg / kg) and EGCG (21.1 mg / kg) respectively 3 times a week, starting on the 30th day of the systemic LPS administration, and the 5th group involved rats, which were injected with co-administered water-soluble form of quercetin and EGCG. It has been found out that the co-effect produced by quercetin and EGCG under systemic and local LPS administration is accompanied with reduced concentration of N-acetylneuraminic acid (NANA) by 31.8 and 32.8% respectively in the soft periodontal tissues compared with values for the animals received separate quercetin and EGCG during the experiment. However, no differences have been detected between the groups exposed to combined or separate action of the above mentioned agents in the experiment when assessing free hydroxyproline (FHP) and glycosaminoglycans (GAGs) content in the soft tissues of periodontium. At the same time combined use of quercetin and EGCG under experimental conditions led to the decrease in the FHP content in the alveolar bone by 24.5 and 20.2% respectively compared with values for the animals received separate quercetin and EGCG. NANA concentration was reduced by 35.0 and 41.3% respectively. Thus, the co-administration of water-soluble form of quercetin and epigallocatechin-3-gallate under systemic and local introducing of S. typhi lipopolysaccharide has been proven to be more effective means for preventing and correcting periodontal connective tissue disruption than this occurs at separate administration of each of the polyphenols.

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Quercetin Potentiates Antiradical Properties of Epigallocatechin-3-Gallate in Periodontium of Rats Under Systemic and Local Administration of Lipopolisaccharide of Salmonella Typhi

2019

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Introduction: There has been demonstrated that pharmaceutical effect of epigallocatechin-3-gallate (EGCG), a polyphenol, which is found in green tea (Camellia sinensis), is implemented through the activation of Nrf2 (Nuclear Factor Erythroid 2-Related Factor 2).The importance of Keap1 / Nrf2 / antioxidant response element (ARE) system is determined by the fact that the state of NF-κB- and АР-1-associated pathways depends on its activity. Recent studies have demonstrated the property of quercetin to suppress ubiquitin-dependent proteolysis of complex of NF-κB and its inhibitory protein IκB. All this provides preconditions to eliminate the potentiality of NF-κB-dependent expression of the number of genes of pro-oxidant and pro-inflammatory proteins. However, co-effect produced by quercetin and EGCG on the oxidative nitrosative stress markers in the periodontal tissues is still unclear. The aim: To investigate the co-effect produced by quercetin and an inducer of the Keap1 / Nrf2 / ARE epigallocatechin-3-gallate on markers of oxidative-nitrosative stress in rats’ periodontium under the systemic and local administration of Salmonella typhi lipopolysaccharide (LPS). Materials and methods: The studies were conducted on 30 white rats of the Wistar line, divided into 5 groups: the 1st included intact animals, the 2nd was made up of animals after their exposure to combined systemic and local LPS administration, the 3rd and 4th groups included animals, which were given injections with water-soluble form of quercetin (corvitin) and EGCG respectively, and the 5th group involved rats, which were injected with co-administered corvitin and EGCG. The formation of superoxide anion radical (.О-2 ) was evaluated by a test with nitro blue tetrazolium using spectrophotometry of the periodontal soft tissue homogenate. The total activity of NO-synthase and concentration of peroxynitrite in the homogenate of the soft components of periodontium were evaluated spectrophotometrically. Results: Co-effect produced by corvitin and EGCG under systemic and local LPS administration is accompanied with reduced О-2 production by NADPH-dependent electron transport chains (microsomal and NOS) by 20.0 % (p<0.05) compared with values for the animals received separate corvitin during the experiment. .О-2 generation by the mitochondrial respiratory chain yielded to comparable data of the 3rd and 4th groups by 27.6 % (p<0.01) and 23.8 % (p<0.05) respectively. No differences were found between the groups exposed to combined or separate action of the above mentioned agents in the experiment when assessing О-2 generation by leukocyte NADPH-oxidase. Combined effect of corvitin and EGCG during systemic and local LSP administration showed the decrease in NOS activity and peroxynitrite concentration in periodontal tissues by 53.3 % (p<0.001) and 27.0 % (p<0.02) compared with the findings in the 3rd group, and by 42.0 % (p<0.01) and 22.3 % (p<0.01) in the 4th group. Conclusions: the co-administration of water-soluble form of quercetin and epigallocatechin-3-gallate under systemic and local introducing of lipopolysaccharide Salmonella typhi has been proven to be more effective means for preventing and correcting oxidative-nitrosative stress in the periodontal tissues than this occurs at separate administration of each of the polyphenols.

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Effect of L-arginine and corvitin on oxidative-nitrosative stress in skin of rats exposed to excessive sodium nitrate

Cited by 4 publications

References 10 publications

Influence of corvitin and metformin on biochemical changes in lacrimal glands of rats during water avoidance stress modeling

Influence of corvitin and metformin on biochemical changes in lacrimal glands of rats during water avoidance stress modeling

Synergistic Effect of Quercetin and Epigallocatechin-3-Gallate as Agents to Prevent Connective Tissue Disintegration in the Periodontium of Rats Under Systemic and Local Administration of Lipopolisaccharide of Salmonella Typhi

Quercetin Potentiates Antiradical Properties of Epigallocatechin-3-Gallate in Periodontium of Rats Under Systemic and Local Administration of Lipopolisaccharide of Salmonella Typhi

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