Effect of L-carnitine and atorvastatin on a rat model of ischemia-reperfusion injury of spinal cord

Hazzaa, Suzan M; Abdou, Asmaa Gaber; Ibraheim, Essam O.; Salem, Esraa A.; Hassan, Mohamad Hasyizan; Abdelrazek, Hesham

doi:10.1080/15321819.2021.1914085

Cited by 7 publications

(7 citation statements)

References 65 publications

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“…Oxidative stress injury plays an important role in SCII. Large amounts of oxygen free radicals are generated during spinal cord ischemia [ 30 , 31 ]. These oxygen radicals can cause changes in the lipid microenvironment such as membrane receptors, membrane proteases, and ion channels, which can cause impaired cell metabolism, cellular edema, and ultimately apoptosis.…”

Section: Discussionmentioning

confidence: 99%

lncRNA HOTAIR Inhibition by Regulating HMGB1/ROS/NF-κB Signal Pathway Promotes the Recovery of Spinal Cord Function

Wang

Long

Yang

et al. 2022

Computational and Mathematical Methods in Medicine

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Spinal cord ischemia-reperfusion injury (SCII) is one of the most serious complications of clinical aortic aneurysm and vascular malformation surgery. Long noncoding RNA (lncRNA) is involved in the progression of SCII, whereas long noncoding RNA HOX transcript antisense RNA (lncRNA HOTAIR) is unclear in SCII. This study is aimed at confirming the role and related mechanism of HOTAIR in SCII. Later on, a model of SCII was established by clamping the aortic arch for 14 minutes. RNA expression of HOTAIR was detected via qRT-PCR at 12 h, 24 h, 36 h, and 48 h after SCII. The Tarlov scoring system and TUNEL assay were used to evaluate neurological function and neuronal apoptosis. Oxidative stress factor levels were assessed according to the instructions of the kit. Inflammatory cytokines were assessed by ELISA. Western blot was used to detect levels of p65, p-p65, I-κBα, and p-I-κBα. We found HOTAIR was raised in SCII rats. si-HOTAIR was able to reverse SCII-induced oxidative stress in SCII rats. The HMGB1 expression was upregulated in SCII tissues and negatively correlated with HOTAIR. HMGB1 was able to partially reverse si-HOTAIR inhibition of oxidative stress, inflammatory injury, and neuronal cell apoptosis in SCII. In addition, the ROS/NF-κB signaling pathway is involved in HOTAIR/HMGB1 regulation of SCII. In a word, HOTAIR inhibition is able to inhibit oxidative stress, inflammatory injury, and neuronal apoptosis in SCII through downregulation of the high mobility group protein B1(HMGB1), which is achieved by inhibiting the ROS/NF-κB signaling pathway. The HOTAIR/HMGB1/ROS/NF-κB molecular pathway may be a new mechanism for the treatment of SCII.

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Section: Discussionmentioning

confidence: 99%

lncRNA HOTAIR Inhibition by Regulating HMGB1/ROS/NF-κB Signal Pathway Promotes the Recovery of Spinal Cord Function

Wang

Long

Yang

et al. 2022

Computational and Mathematical Methods in Medicine

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“…As a cofactor, LC involved in medium and long-chain fatty acids to pass into the mitochondrial matrix. Also, studies demonstrated that LC is an antioxidant and anti-inflammatory aspects in tissues without any side effect (10,11,(17)(18)(19). Since there are so little studies on the effects of LC tendon recovery, we aimed to research the effects of LC on an Achilles tendon injury model.…”

Section: Discussionmentioning

confidence: 99%

The Effect of L-Carnitine on Experimental Achilles Tendon Injury

Kasar,

Demirci,

Hünler

et al. 2023

Muscle Ligaments and Tendons J

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Background. L-carnitine (LC), which has anti-inflammatory and antioxidant effect, is especially consumed by sportmans to increase muscle mass recently. Therefore, the functional, histopathological effects of LC on experimental Achilles tendon injury were investigated in this study. Methods. Forty male Wistar Albino rats were randomly divided into four groups (n = 10). A tendon injury model was created by clamping the left Achilles tendon of all rats except the control group. Tendon Injury Group (TIG) and the Control group did not receive LC treatment. LC (100 mg/kg/day, intraperitoneally) was started to administer one week before the injury model in pre-LC group and the same day of injury in post-LC group following the establishment of model for four weeks. After the tendon injury and at the end of the experiment, rats in all groups were subjected to a Gait test (GT) twice. On the 30 th day of injury, the rats were sacrificed, and left Achilles tendons were dissected for histopathological analysis. Results. Initial GT data were significantly different between the control and other groups (p < 0.001). The final GT data were significantly different only between the Control and TIG (p = 0.002). Histopathological examination revealed that neovascularization, fibroblastic activity, and collagen fibril sequencing parameters were found to be significantly different amongst the TIG and pre-LC group, and post-LC group (p < 0.05). Conclusions. LC contributes to the tendon healing by histopathologically and functionally.

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“…According to Kaur et al ( 2013 ), GbE, and some of its constituents, such as ginkgolide B and bilobalide, protected neurons against apoptotic and excitotoxic damage. Also, Babu et al ( 2011 ) and Hazzaa et al ( 2021 ) reported the neuroprotective role of LC through its antioxidant and anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

Anti-kindling effect of Ginkgo biloba leaf extract and L-carnitine in the pentylenetetrazol model of epilepsy

Essawy

El-Sayed

Tousson

et al. 2022

Environ Sci Pollut Res

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Epilepsy is one of the most common serious brain disorders, affecting about 1% of the population all over the world. Ginkgo biloba extract (GbE) and L-carnitine (LC) reportedly possess the antioxidative activity and neuroprotective potential. In this report, we investigated the possible protective and therapeutic effects of GbE and LC against pentylenetetrazol (PTZ)-induced epileptic seizures in rat hippocampus and hypothalamus. Adult male albino rats were equally divided into eight groups: control, GbE (100 mg/kg), LC (300 mg/kg), PTZ (40 mg/kg), protective groups (GbE + PTZ and LC + PTZ), and therapeutic groups (PTZ + GbE and PTZ + LC). The oxidative stress, antioxidant, and neurochemical parameters, viz., malondialdehyde (MDA), nitric oxide (NO), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), acetylcholine esterase (AchE), dopamine (DA), norepinephrine (NE), and serotonin (5-HT), in the hippocampal and hypothalamic regions have been evaluated. PTZ injection leads to an increase in the seizure score, the levels of MDA and NO, and to a decrease in the activity of GSH, SOD, CAT, and GPx. Besides, monoamine neurotransmitters, DA, NE, and 5-HT, were depleted in PTZ-kindled rats. Furthermore, PTZ administration caused a significant elevation in the activity of AchE. Hippocampal and hypothalamic sections from PTZ-treated animals were characterized by severe histopathological alterations and, intensely, increased the ezrin immunolabeled astrocytes. Pre- and post-treatment of PTZ rats with GbE and LC suppressed the kindling acquisition process and remarkably alleviated all the aforementioned PTZ-induced effects. GbE and LC have potent protective and therapeutic effects against PTZ-induced kindling seizures via the amelioration of oxidative/antioxidative imbalance, neuromodulatory, and antiepileptic actions.

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Effect of L-carnitine and atorvastatin on a rat model of ischemia-reperfusion injury of spinal cord

Cited by 7 publications

References 65 publications

lncRNA HOTAIR Inhibition by Regulating HMGB1/ROS/NF-κB Signal Pathway Promotes the Recovery of Spinal Cord Function

lncRNA HOTAIR Inhibition by Regulating HMGB1/ROS/NF-κB Signal Pathway Promotes the Recovery of Spinal Cord Function

The Effect of L-Carnitine on Experimental Achilles Tendon Injury

Anti-kindling effect of Ginkgo biloba leaf extract and L-carnitine in the pentylenetetrazol model of epilepsy

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