Effect Of L-Citrulline And Ranitidine On Indomethacin-Induced Gastric Ulcer In Male Albino Wister Rats

Abstract

Aim: to investigate the effect of L-citrulline and ranitidine (RAN) on indomethacin-induced gastric ulcer in male albino Wister rats. Methods: 50 male albino Wister rats were divided into 5 groups (10rats each): control received normal saline, indomethacin (IND) group; in which gastric ulceration was induced by a single dose of IND (30mg/kg) intragastrically, IND+RAN group; pretreated with RAN (50mg/kg) intragastrically, IND+ L-citrulline group; pretreated with Lcitrulline (900mg/kg) intragastrically, IND+RAN+L-citrulline group; received co-treatment with RAN and L-citrulline intragastrically. At the end of experiments, ulcer index (U.I.) and preventive index (P.I.) and gastric juice pH, volume of gastric juice, free and total gastric acidity, pepsin activity and mucin content were assessed. Gastric homogenates were used for determination of Malondialdehyde (MDA), (nitrite/nitrate) NO content, prostaglandin E2 (PGE2), myeloperoxidase (MPO) activity and heme oxygenase (HO) activity. Results: pretreatment with either RAN or L-citrulline alone significantly reduced U.I. and also significantly reduced gastric juice volume, free and total acidity, pepsin activity, and gastric MDA with concomitant significant increase in pH of gastric juice. But, RAN insignificantly alter mucin content, NO content, PGE2, MPO activity and HO activity compared to IND group. While, pretreatment with L-citrulline alone significantly increased mucin content, NO content, PGE2, HO activity and significantly decreased MPO activity compared to either IND group or RAN group. Coadministration of RAN and L-citrulline resulted in more significant reduction of U.I. providing 87.30% prevention, and also more significant reduction of gastric juice volume, free and total acidity, pepsin activity, gastric MDA and MPO activity with concomitant more significant increase in pH of gastric juice and NO content, PGE2 synthesis and HO activity compared to either IND group or RAN groups. Conclusions: It could be concluded from this present study that the combination of L-citrulline and RAN afford a good gastroprotective potential against the gastric ulceration induced by IND better than each drug alone.

A Retrospective Trial Exploring Erzhu Yiren Decoction in Gastric Ulcer with Spleen Deficiency and Dampness‐Heat

“…Although its pathogenesis has yet been elucidated, it is considered a consequence of the overburdened resistance and downgraded self-repair capability of the gastric mucosa after damages [1][2][3]. Current Western medicine treatment for gastric ulcers mainly employs drugs that inhibit gastric acid and protect the gastric mucosa and anti-Hp drugs which alleviate the clinical symptoms of patients but also lead to obvious side effects, resulting in a predisposition to more serious diseases [4,5]. Moreover, clinical studies have shown that Western medicine treatment fails to optimize the quality of ulcer healing (QOUH), with the recurrence rate reaching 80% 1 year after drug withdrawal [6,7], which is indicative of an unsatisfactory overall efficacy.…”