Effect of L-Lactic Acid on Calcium Absorption in Rats Fed Omeprazole.

Chonan, Osamu; Takahashi, Rie; Yasui, Hisako; Watanuki, Masaaki

doi:10.3177/jnsv.44.473

Cited by 51 publications

(47 citation statements)

References 19 publications

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“…This solubility of the calcium salts is pH-dependent, meaning that low pH is mandatory for calcium salt dissolution (23). Therefore, some propose that the blockage of acid secretion by PPIs hinders absorption of calcium which results in an increased risk of bone fracture (13). This finding has also been noted in a small randomized crossover trial by O'Connell et al in which a reduction of fractional calcium absorption was observed in those taking PPIs (14).…”

Section: Discussionsupporting

confidence: 59%

“…The use of PPIs could increase the risk of bone fractures by affecting calcium homeostasis, which can either be due to inhibition of calcium absorption (13,14) or to the induction of hyperparathyroidism secondary to the development of hypergastrinemia (15,16), although controversy remains. Since PPIs block the acid secretion not only in parietal cells but also in osteoclasts, an essential step in bone resorption (17)(18)(19), they could theoretically decrease the risk of bone fractures and be osteoprotective.…”

Section: Introductionmentioning

confidence: 99%

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Effect of omeprazole on the expression of transcription factors in osteoclasts and osteoblasts

Hyun

Chun²,

Keum³

et al. 2010

Int J Mol Med

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Abstract. The use of proton pump inhibitors (PPIs) seems to be related to increased fracture risk but the mechanism is unclear. In an effort to clarify the mechanism, we evaluated the effect of omeprazole, a representative of the PPIs, on the expression of transcription factors in osteoclasts and osteoblasts. Murine RAW264.7 and MC3T3-E1 cells were used for osteoclast and osteoblast analysis, to which various concentrations of omeprazole were added. RAW264.7 cells with ≥3 nuclei were considered tartrate-resistant acid phosphatase (TRAP)-positive, i.e. activated osteoclasts. Expressions of the calcitonin receptor (CTR), c-fos, nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), and matrix metalloproteinase (MMP)-9 mRNA in osteoclasts were evaluated. Gene expression of osteocalcin and of the osteoprotegerin/receptor activator of NF-κB ligand (OPG/RANKL) ratio in osteoblasts was examined and Western blotting of NFATc1 was performed. Treating the osteoclasts with increasing doses of omeprazole did not affect TRAP positivity, but significantly decreased the expressions of CTR, c-fos, NFATc1, and MMP-9 regardless of the omeprazole concentration. The expression of osteocalcin and of the OPG/RANKL ratio in osteoblasts was augmented with increasing omeprazole concentrations. The result of the Western blot analysis with NFATc1 was similar to that of the expression of NFATc1 mRNA. Omeprazole decreased the activation of osteoclasts but increased that of osteoblasts in vitro, in part causing an osteopetrosis-like effect. Together with the effect of omeprazole on calcium homeostasis, increased fracture risk may be due to the osteopetrorickets-like effect of omeprazole.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Effect of omeprazole on the expression of transcription factors in osteoclasts and osteoblasts

Hyun

Chun²,

Keum³

et al. 2010

Int J Mol Med

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“…The significance of gastric acid secretion regarding dietary calcium absorption is thus controversial. Our previous findings also suggested that the gastric acidity induced by dietary LA is not the only factor in the stimulatory effect of LA on calcium absorption (11). In the present study, we reexamined the effect of dietary LA on calcium absorption to identify the mechanism of LA-enhanced calcium absorption.…”

Section: Body Weights and Food Intakesmentioning

confidence: 65%

“…* To whom correspondence should be addressed. We previously demonstrated that the inhibition of gastric acid secretion by dietary omeprazole (OM), a proton pump inhibitor, decreased the apparent calcium absorption and that dietary L-lactic acid (LA), which is present in fermented products such as yogurt, prevented the inhibition of calcium absorption in rats fed OM (11) .…”

Section: Discussionmentioning

confidence: 99%

Effect of L-Lactic Acid on the Absorption of Calcium in Gastrectomized Rats.

Chonan

Takahashi

Yasui

et al. 1998

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe effect of dietary L-lactic acid (LA) (0.5, 10, or 2.5g/100g of diet) on the absorption of calcium in gastrectomized rats was evaluated for 28d. Calcium phosphate was used as a source of calcium. The apparent calcium absorption ratio and the calcium contents of the femur and tibia in gastrectomized rats fed the control diet were significantly less than those in sham-operated rats. In the gastrectomized rats, the apparent calcium absorption ratio and the calcium contents of bone in the rats fed the lower doses of LA diets (LA 0.5 or 1.0g/100g of diet) were not affected; however, the apparent calcium absorption ratio and the calcium contents of bone in the rats fed the highest doses of LA diet (LA 2.5g/100g of diet) were greater than those in gastrectomized rats fed the control diet. Dietary LA (2.5g/100g of diet) also enhanced the phosphorus absorption and bone phosphorus content in the gastrectomized rats. We speculated that the highest dose of dietary LA might be associated with the dissolving of a water-insoluble form of calcium salt in the diet, thereby facilitating the calcium absorption and resulting in increased bone calcium content in gastrectomized rats.

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“…It is thought that PPIs reduce the production and secretion of hydrochloric acid in stomach, increase the pH in the stomach and inhibit absorption of insoluble calcium in the small intestine, thus leading to malabsorption of calcium phosphate and bone metabolism disorder (15,16). PPIs also reportedly inhibit bone resorption by osteoclasts (17,18).…”

Section: Introductionmentioning

confidence: 99%

Effects of rabeprazole on bone metabolic disorders in a gastrectomized rat model

et al. 2016

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Abstract. Proton pump inhibitors (PPIs) are frequently prescribed to patients with gastroesophageal reflux disease; however, the number of bone fractures reportedly increased in these patients. Although PPIs have been shown to inhibit the bone resorption by osteoclasts, the effect of PPIs on skeletal metabolism remains controversial. The aim of the present study was to determine the effect of the PPI rabeprazole on skeletal metabolism using gastrectomized rats. Male Wistar rats were divided into four groups: i) Sham-surgery (n=15); ii) total gastrectomy (TG) control (n=20); iii) TG plus rabeprazole (n=20); and iv) TG plus the bisphosphonate minodronic acid (n=20). Twenty-two weeks after TG, the rats were sacrificed, and bone mineral density (BMD), bone strength and markers for bone metabolism were measured. Compared with the control group (50.0±8.1%), the TG-induced decrease in BMD was significantly ameliorated in the rabeprazole group (56.5±7.5%) and the minodronic acid group (59.0±6.0%). However, rabeprazole did not improve bone strength. In conclusion, rabeprazole does not appear to exacerbate bone metabolic disorders in gastrectomized rats, but rather ameliorates the TG-induced BMD decrease.

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Effect of L-Lactic Acid on Calcium Absorption in Rats Fed Omeprazole.

Cited by 51 publications

References 19 publications

Effect of omeprazole on the expression of transcription factors in osteoclasts and osteoblasts

Effect of omeprazole on the expression of transcription factors in osteoclasts and osteoblasts

Effect of L-Lactic Acid on the Absorption of Calcium in Gastrectomized Rats.

Effects of rabeprazole on bone metabolic disorders in a gastrectomized rat model

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