Effect of Lactobacillus fermentum alone, and in combination with zinc(II) propionate on Salmonella enterica serovar Düsseldorf in Japanese quails

Abstract

The influence of zinc(II) propionate on the efficacy of Lactobacillus fermentum against Salmonella enterica serovar Düsseldorf was tested in Japanese quails. Twenty one 3-day old Japanese quails were divided into 3 groups each consisting of 7 birds and inoculated orally: (i) group A (control) with Rogosa broth; (ii) group B with rifampicin-resistant L. fermentum (2.5 × 10 7 CFU/animal); and (iii) group C with rifampicin-resistant L. fermentum cultured in Rogosa broth supplemented with zinc(II) propionate (1 g Zn 2+ /L of broth; i.e. 2.5 × 10 7 CFU/animal and 0.1 mg Zn 2+ /animal). After 16 hours all birds were infected with a single dose of S. enterica serovar Düsseldorf (5.8 × 10 7 CFU/animal). During the next 6 days the chicks received the same inoculations as they had received earlier through the medium of drinking water. The viable counts of Salmonella, rifampicin-resistant lactobacilli and total aerobes in the feces and cecal content, zinc concentration in the cecal content and growth performance were evaluated. L. fermentum alone, and in combination with Zn(II) propionate significantly reduced shedding of Salmonella in the feces and also the amount of salmonellae present in cecal content as compared to control. The numbers of Salmonella in group C in all collections were lower than in group B, but a significant difference (p < 0.05) was noted only 24 h after infection. The viable counts of rifampicin-resistant lactobacilli were similar in groups B and C and numbers of total aerobes were reduced in these groups compared with control. L. fermentum and its combination with zinc(II) propionate increased daily weight gains in the chicks in comparison with control. Zinc concentration in the control and C group was 34.9 ± 6.2 mg · kg −1 and 676.3 ± 106.6 mg · kg −1 of cecal content, respectively.

Effect of Inorganic Zinc on Selected Immune Parameters in Chicken Blood and Jejunum after A. galli Infection

“…Zinc (Zn) is an essential mineral involved in many biochemical processes and associated with a wide range of physiological disorders, including weight loss, growth retardation, and nervous and immune system disorders [14]. In addition, zinc is an essential factor in the gene expression of proteins required for growth and development, maintaining cell wall integrity, free radical sequestration, and protection against lipid peroxidation [15].…”

The effect of Limosilactobacillus fermentum 2i3 and 0.6% addition of humic substances on production parameters and the immune system of broilers

“…The probiotic strain Lactobacillus fermentum 2I3 (new nomeclature: Limosilactobacillus fermentum ) was isolated from the intestinal contents of a healthy domestic pheasant at the Department of Microbiology and Immunology UVLF in Košice. The strain was tested in several in vitro and in vivo experiments, which confirmed its probiotic properties ( Koščová et al 2006 ; Mudroňová et al 2006 ; Englerová et al, 2018 ; Harčárová et al, 2021 ). The strain L. fermentum 2I3 was cultured in MRS broth (Merck, Germany) for 18 h at 37°C in a shaking water bath.…”

Newly isolated Limosilactobacillus reuteri B1/1 modulates cell response in porcine ex vivo model mirroring the gut

“…-slimy), which also includes the strains L. reuteri and L. fermentum, is characterized by the property of most strains of this genus to produce exopolysaccharides (EPS) from sucrose to promote bio lm formation on epithelia in the gut (Zheng et al, 2020). In this study L. fermentum CCM 7158 was used as an indicator LAB strain since it is included in the Czech Collection of Microorganisms (CCM) and its characteristics have been widely studied both in in vitro and in vivo conditions in previous studies (Capcarova et al, 2010;Hrnčár et al, 2021Hrnčár et al, , 2014Mudroňová et al, 2006). An interesting nding in our work is that simultaneous treatment of MDM cells with LPS and treatment of IPEC-J2 with probiotic strain L. fermentum CCM 7158 led to downregulation of mRNA for CLDN-1 and OCLN in intestinal cells.…”