Effect of Lactobacillus reuteri on Gingival Inflammation and Composition of the Oral Microbiota in Patients Undergoing Treatment with Fixed Orthodontic Appliances: Study Protocol of a Randomized Control Trial

Abstract

The effect of probiotics in improving or maintaining oral health in orthodontic patients is understudied. The aim of this study is to evaluate the effect of probiotic administration in addition to tooth brushing on clinical gingival inflammation, plaque formation, subgingival microbiota composition, and salivary biomarkers of inflammation in adolescents with fixed orthodontic appliances. The present study is a 6-month, double-blind, two-arm, placebo-controlled, single-center trial, in which 116 adolescent volunteers aged 12–16 years will be recruited from the patients of the orthodontics clinic of the University Hospital of Lille, France. Subjects who meet the eligibility criteria will be allocated to one of the following groups: (i) control: two placebo lozenges per day for 90 days together with regular oral hygiene, (ii) test: two probiotic lozenges per day for 90 days together with regular oral hygiene. Clinical assessment and biological sample collection will be performed at baseline, 3 and 6 months. In addition, compliance outcomes and adverse events will be monitored.

Short- term effect of probiotic Lactobacillus reuteri consumption on the salivary microbiome profile of subjects undergoing orthodontic treatment with fixed appliances

“…The use of probiotics as an adjunct therapy has been suggested for various diseases including periodontitis. Overall, the administration of probiotics, either as an adjunct to mechanical plaque control or as a single intervention, has yielded conflicting results [ 38 ]. Some studies have proven the advantages of probiotic consumption in reducing the extent of gingival inflammation and the amount of plaque [ 39 , 40 ].…”

Analgesic Potential Comparison Between Piperine-Combined Curcumin Patch and Non-Piperine Curcumin Patch: A Pragmatic Trial on Post-Cleft Lip/Palate Surgery Pediatric Patients

“…Saliva collection and sample preparation were performed according to the manufacturer’s instruction sand as described in the previous study. 39 It was performed by our field researcher under the supervision of a clinical staff of the Clinical Pathology Laboratory of Hasan Sadikin Hospital. Once the saliva sample was collected, the sample was brought by the clinical staff to the Clinical Pathology Laboratory of Hasan Sadikin Hospital, Bandung, Indonesia, and was analyzed by using the Enzyme-linked of Immunosorbent Assay (ELISA) technique.…”

The antimicrobial effect of Limosilactobacillus reuteri as probiotic on oral bacteria: A scoping review

“…14 In terms of oral health, Limosilactobacillus reuteri has been extensively researched and is considered one of the most studied probiotics. 15 Limosilactobacillus reuteri is known for its ability to control oral microbiota through reuterin, a wide-spectrum antimicrobial agent. 16 However, some studies discovered that the Limosilactobacilluss reuteri probiotic should not be recommended as an adjunct treatment for oral infection as there were no microbiota alterations between the test and control groups.…”