Effect of Lactobacillus rhamnosus cells against specific and native fish spoilage bacteria and their spoilage indices on Asian seabass fish chunks

Abstract

Journal of Environmental Biology Interpretation :The present study aimed at evaluating probiotic bacterial cells, Lb. rhamnosus by coating on seabass fish meat chunks against its spoilage indices,native and specific fish spoilage bacteria.Seabass fish fillets were cut into chunks, washed and taken into petri plates. Scores were made using sterile blade. Lb. rhamnosus cells were coated on the chunks using micro pipette and tested for antagonism against fish-borne specific spoilage bacteria such as Shewanella putrefaciens and Pseudomonas fluorescens through "Deferred antagonism assay". Spent culture of Lb. rhamnosus was assayed for antagonism through "Agar-well diffusion assay". The changes were estimated for two days following "Conway micro diffusion" method.