Effect of lactoferrin-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres on osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs)

Kim, Sung Eun; Yun, Young Pil; Shim, Kyu Sik; Park, Kyeongsoon; Choi, Sung Wook; Suh, Dong Hun

doi:10.1016/j.colsurfb.2014.06.057

Cited by 39 publications

(51 citation statements)

References 47 publications

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“…After adhering Hep-DOPA, the N1s and S2p content of Hep-PMS increased compared to PMS alone. Our previous studies demonstrated that PMS, polycaprolactone (PCL) fibers and titanium (Ti) modified with Hep-DOPA showed increased N1s and S2p content compared to PMS, PCL fibers, and Ti only [9,19,34]. Moreover, after immobilizing BMP-2 on PMS and Hep-PMS, N1s and S2p content on PMS and Hep-PMS containing BMP-2 increased compared to that on Hep-PMS.…”

Section: Discussionmentioning

confidence: 86%

“…The objective of this study was to fabricate porous microspheres and to subsequently immobilize BMP-2 to demonstrate BMP-2 as a candidate material for the regeneration of bone defects. In this study, we used a fluidic device method described by Kim et al [9] and Choi et al [31] to fabricate microspheres with interconnected pores. The advantages of a fluidic device method include easily controllable size, porosity, and the shape of the microspheres.…”

Section: Discussionmentioning

confidence: 99%

“…The mixture was dialyzed (MWCO = 2000, Spectrum ® ) against acidified distilled water (DW) for two days and lyophilized. To fabricate porous microspheres (PMS) immobilized with Hep-DOPA and BMP-2, a fluidic device method was used as previously described by Kim et al [9]. PLGA (140 mg) was dissolved in 7 mL of dichloromethane solution.…”

Section: Fabrication Of Porous Microsphere By Heparin-dopamine (Hep-dmentioning

confidence: 99%

“…Using injectable scaffolds for bone defects does offer some advantages, such as increased ease of the operation using 10-to 16-gauge needle syringes, shorter operative times, minimal scarring, and increased patient satisfaction. Moreover, as an injectable material, the microspheres with interconnective pores between the surface and interior are a good delivery vehicle not only for cells, but for the sustained release of various drugs or proteins as well [9][10][11]. Accordingly, microspheres have been indicated as a potential material for clinical applications requiring carriers for drugs, proteins and cells [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Kim

Yun

Shim

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Fabrication Of Porous Microsphere By Heparin-dopamine (Hep-dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Kim

Yun

Shim

et al. 2015

Colloids and Surfaces B: Biointerfaces

Self Cite

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“…22 Tf is used as a tumor-targeting ligand because Tf receptors are overexpressed in several human carcinomas including brain, breast, and ovary cancers. 23,24 In our recent work, Lf-conjugated nanoparticles were developed as the specific and multifunctional magnetic resonance imaging contrast agent for the diagnosis of brain glioma.…”

Section: Introductionmentioning

confidence: 99%

Novel lactoferrin-conjugated amphiphilic poly(aminoethyl ethylene phosphate)/poly(L-lactide) copolymer nanobubbles for tumor-targeting ultrasonic imaging

Luo¹,

Liang²,

Zhang³

et al. 2015

IJN

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In the study reported here, a novel amphiphilic poly(aminoethyl ethylene phosphate)/poly(L-lactide) (PAEEP-PLLA) copolymer was synthesized by ring-opening polymerization reaction. The perfluoropentane-filled PAEEP-PLLA nanobubbles (NBs) were prepared using the O 1 /O 2 /W double-emulsion and solvent-evaporation method, with the copolymer as the shell and liquid perfluoropentane as the core of NBs. The prepared NBs were further conjugated with lactoferrin (Lf) for tumor-cell targeting. The resulting Lf-conjugated amphiphilic poly(aminoethyl ethylene phosphate)/poly(L-lactide) nanobubbles (Lf-PAEEP-PLLA NBs) were characterized by photon correlation spectroscopy, polyacrylamide gel electrophoresis, Fourier transform infrared spectroscopy, and transmission electron microscopy. The average size of the Lf-PAEEP-PLLA NBs was 328.4±5.1 nm, with polydispersity index of 0.167±0.020, and zeta potential of −12.6±0.3 mV. Transmission electron microscopy imaging showed that the Lf-PAEEP-PLLA NBs had a near-spherical structure, were quite monodisperse, and there was a clear interface between the copolymer shell and the liquid core inside the NBs. The Lf-PAEEP-PLLA NBs also exhibited good biocompatibility in cytotoxicity and hemolysis studies and good stability during storage. The high cellular uptake of Lf-PAEEP-PLLA NBs in C6 cells (low-density lipoprotein receptor-related protein 1-positive cells) at concentrations of 0–20 µg/mL indicated that the Lf provided effective targeting for brain-tumor cells. The in vitro acoustic behavior of Lf-PAEEP-PLLA NBs was evaluated using a B-mode clinical ultrasound imaging system. In vivo ultrasound imaging was performed on tumor-bearing BALB/c nude mice, and compared with SonoVue ® microbubbles, a commercial ultrasonic contrast agent. Both in vitro and in vivo ultrasound imaging indicated that the Lf-PAEEP-PLLA NBs possessed strong, long-lasting, and tumor-enhanced ultrasonic contrast ability. Taken together, these results indicate that Lf-PAEEP-PLLA NBs represent a promising nano-sized ultrasonic contrast agent for tumor-targeting ultrasonic imaging.

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Bisphosphonate‐Functionalized Scaffolds for Enhanced Bone Regeneration

Cui

Zhu

et al. 2019

Adv Healthcare Materials

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Bone defect healing is an intricate but methodical process that restores bone integrity and represents an intrinsic capacity of The local sustained release of bioactive substances are attracting increasing attention in bone tissue engineering, which is beneficial to bone tissue formation and helps to improve the bone ingrowth ability of a scaffold. Bisphosphonates (BPs), as a representative kind of osteoclast inhibitors, are proven to possess excellent osteogenic induction capability. Accordingly, various physical and chemical strategies are developed to functionalize bone tissue scaffolds with BPs to achieve controlled release profiles. Compared with traditional treatment modalities, local release of BPs from these composite scaffolds will contribute to continuous bone integration without the risk of many complications. This review explores the molecular mechanisms of BPs on bone metabolism and analyzes the appropriate concentrations of BPs that promote bone regeneration. The advanced BP loading strategies, implant modification technologies, and BP-loaded composite scaffolds based on different matrices are summarized. Finally, the latest advances and the future development of BP-modified scaffolds for enhanced bone regeneration are discussed. This article provides leading-edge design strategies of the BP-functionalized bone engineering scaffolds for improved bone repairability.

show abstract

Effect of lactoferrin-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres on osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs)

Cited by 39 publications

References 47 publications

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Novel lactoferrin-conjugated amphiphilic poly(aminoethyl ethylene phosphate)/poly(L-lactide) copolymer nanobubbles for tumor-targeting ultrasonic imaging

Bisphosphonate‐Functionalized Scaffolds for Enhanced Bone Regeneration

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