Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa

Corcuera, B.D.; Marigorta, Pilar; Sagüés, A.; Saiz-Cidoncha, F.; Pérez-Gutiérrez, J.F.

doi:10.1016/j.theriogenology.2007.01.002

Cited by 38 publications

(41 citation statements)

References 41 publications

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“…Many substances have been tested to determine whether they enhance the resistance of spermatozoa to the stress of freezing and thawing in several species, including the pig (Roca et al 2004, 2005b, Breininger et al 2005, Bathgate et al 2006, Corcuera et al 2007, De Mercado et al 2009, Hu et al 2009). Pentoxifylline, a methylxanthine derivate, is an inhibitor of phosphodiesterase.…”

Section: Introductionmentioning

confidence: 99%

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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This study evaluated whether pentoxifylline added to freezing and thawing extenders influenced the function of boar spermatozoa. In Experiment 1, pooled ejaculated sperm-rich fractions were frozen in 0.5 ml straws after dilution in extender supplemented with pentoxifylline to a final concentration of 0, 2, 4, 8, 16 or 32 mM. The addition of 4, 8, 16 and 32 mM pentoxifylline to the freezing extender significantly decreased the progressive and total motility of spermatozoa. The percentage of viable spermatozoa with intact acrosomes as well as the penetration rate and the efficiency of fertilisation were significantly lower in pentoxifylline-treated groups compared with the untreated control. In Experiment 2, a pool of three straws with 'good' post-thaw sperm quality parameters and another three straws with 'poor' sperm quality were diluted in extender with 0, 1, 2, 4, 8, 16 or 32 mM pentoxifylline. Post-thaw samples with both 'good' and 'poor' sperm quality with 0, 2, 4, 8 and 16 mM were used to assess IVF parameters. The addition of pentoxifylline to post-thaw extender did not improve the post-thaw motility or viability of spermatozoa compared with the control. The in vitro penetration was higher (P!0.05) than the control for oocytes fertilised with spermatozoa that were thawed and incubated in extender with 4, 8 and 16 mM pentoxifylline. However, no differences were observed in the efficiency of fertilisation. We conclude that pentoxifylline, as a supplement added to the freezing extender, has a deleterious effect and that it does not improve the survival or in vitro fertilising efficiency of frozen-thawed boar spermatozoa when added after thawing.

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Section: Introductionmentioning

confidence: 99%

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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“…Membrane phospholipid, in turn, stabilized membrane lipid bilayer and maintained sperm membrane integrity [25]. In addition, the effect of sugar concentration used in the semen extender was reported [11]. Corcuera et al [11] found that the use of 12% lactose-based extender (osmolality 360 mOsm/kg) resulted in a higher post-thawed sperm motility and normal apical ridge than the use of 11% and 14% lactose extenders.…”

mentioning

confidence: 99%

“…In addition, the effect of sugar concentration used in the semen extender was reported [11]. Corcuera et al [11] found that the use of 12% lactose-based extender (osmolality 360 mOsm/kg) resulted in a higher post-thawed sperm motility and normal apical ridge than the use of 11% and 14% lactose extenders. These studies revealed that both type and concentration of sugar used in the extender were important to the cryopreservation of boar semen.…”

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confidence: 99%

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

2012

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ABSTRACT. The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolkbased extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (P<0.05) and fructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen. Boar semen cryopreservation greatly facilitates the distribution of valuable and desirable genes because the frozen semen can be transported over a long distance; and a stock of frozen semen enables the use of boar that is dead or ill [3]. However, the use of frozen-thawed (FT) boar semen is limited due to the low fertility outcomes compared to the extended fresh semen [9]. During cryopreservation, both physical and chemical factors including the rapid change in temperature or thermal stress, oxidative stress and osmotic stress lead to the damage of the sperm plasma membrane [19]. In general, the egg yolk-based freezing extender of boar semen consists of cryoprotectant, sugar, buffer and antibiotic [16]. Glycerol is the most common cryoprotectant used in the egg yolk-based semen extender for cryopreservation of the boar semen [14] and egg yolk is regarded as a non-permeable cryoprotectant. Sugar is mainly an energy source of the boar sperm. Furthermore, it protects the boar sperm from dehydration and intracellular ice formation during the semen freezing process [27]. Different types of sugar, e.g., trehalose, lactose, fructose, have been used in the freezing extender of boar semen [15,18].Recently, special interest on researches concerning with the energy utilization of the boar sperm have been noticed [13,15,18,20]. In general, the boar sperm is able to utilize a variety of substrates to obtain energy. Generally, monosaccharides are more common energy source used in extended fresh boar semen than disaccharides [20]. However, lactose (disaccharide) is commonly used for the cryopreservation of boar semen by many researchers [8,9,16,17]. In our previous studies [10,17] [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24]. In the boar, information associated with the effect of sugar types used in egg yolk-based semen extender on the FT semen qualities is limited. Therefore, the objective of the present study was to determine the effect of different sugar types (lactose, fructose, glucose and sorbitol) used in egg yolk-based semen extender on the sperm motility, sperm viability, acrosome integrity a...

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“…A total of 22 collections were done using vinyl gloves. Only the rich fraction (Corcuera et al, 2007) was used. The semen collecting was done at 7 day intervals for every boar.…”

Section: Methodsmentioning

confidence: 99%

Osmolarity of Coconut Water (Cocos nucifera) Based Diluents and their Effect Over Viability of Frozen Boar Semen

Luzardo¹,

Gamboa²,

López³

et al. 2010

American J. of Animal and Veterinary Sciences

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Problem statement: Boar sperm cells are sensitive to the freezing process, which compromises viability of frozen-thawed sperm. In a constant search for minimizing or suppressing sperm cell damage caused by the temperature and osmolarity changes during the freezing process, crioprotective and antioxidant substances have been added to the freezing media, such as coconut water, in order to increase the viability of frozen-thawed swine semen. The addition of any substance to the freezing diluent, directly affects osmolarity of the media, which can have positive or negative effects over the sperm cell. Approach: There are no published studies currently that indicate the effect of adding coconut water over the osmolarity of freezing media and their effect over viability of sperm cells, therefore, the objective of the present study was to evaluate the effect of coconut water based diluents osmolarity over the Motility (Mot), Acrosome Integrity (AI), Membrane Integrity (MI) and Mitochondrial Activity (MA) of thawed boar sperm cells. The treatments used were control T1 (LEY with bidistilled water + LEYGO) with an osmolarity range of 296-368 mOsmol Kg −1 , T2 (LEY and deionized coconut water + LEYGO) between 381 and 480 mOsmol Kg −1 and T3 (LEY and in natura coconut water + LEYGO) between 519 and 1041 mOsmol Kg −1 . The Westendorf modified method was the freezing method used. The obtained data were statistically analyzed by GLM, using the SAS software (SAS, 2000). Results: A significant difference was observed on T2 compared to T1 regarding Mot 41.9 Vs 36.9% and MI 58.0 Vs 50.2. T3 had a detrimental effect on all studied variables. Conclusion: Under our study conditions, the osmolarity range of T2, due to the non ionic solutes content, contributed to improve the viability of frozen-thawed sperm cells.

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Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa

Cited by 38 publications

References 41 publications

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

Osmolarity of Coconut Water (Cocos nucifera) Based Diluents and their Effect Over Viability of Frozen Boar Semen

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