Effect of Lantana camara Ethanolic Leaf Extract on Survival and Migration of MDA-MB-231 Triple-Negative Breast Cancer Cell Line

Pal, A.; Sanyal, S.; Das, S.; Sengupta, T.K.

doi:10.1016/j.hermed.2023.100837

Cited by 2 publications

(1 citation statement)

References 59 publications

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“…Around 15% of breast tumors have triple-negative breast cancers (TNBC), which are often distinguished by a poor prognosis and high rates of proliferation and metastasis (Dai et al 2017;Giovannelli et al 2019). It was found that epidermal growth factor receptor (EGFR) was overexpressed TNBC, which was inversely connected with clinical prognosis, and that it was intimately associated with the proliferation, invasion, and vascular development of cancer (Pal et al 2024). TNBC is a prominent cause of cancer deaths in women due to its aggressive nature and a lack of effective therapy methods (Berger et al 2021;Akter et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Functionalized siRNA-chitosan nanoformulations promote triple-negative breast cancer cell death via blocking the miRNA-21/AKT/ERK signaling axis: in-silico and in vitro studies

Abdulmalek,

Saleh,

Shahin

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Oncogenic microRNA (miRNA), especially miRNA-21 upregulation in triple-negative breast cancer (TNBC), suggests a new class of therapeutic targets. In this study, we aimed to create GE11 peptide-conjugated small interfering RNA-loaded chitosan nanoparticles (GE11-siRNA-CSNPs) for the targeting of EGFR overexpressed TNBC and selectively inhibit miRNA-21 expression. A variety of in-silico and in vitro cellular and molecular studies were conducted to investigate the binding affinities of specific targets used as well as the anticancer efficacies and mechanisms of GE11-siRNA-CSNPs in TNBC cells. An in-silico assessment reveals a distinct binding affinity of miRNA-21 with siRNA as well as between the extracellular domain of EGFR and synthesized peptides. Notably, the in vitro results showed that GE11-siRNA-CSNPs were revealed to have better cytotoxicity against TNBC cells. It significantly inhibits miRNA-21 expression, cell migration, and colony formation. The results also indicated that GE11-siRNA-CSNPs impeded cell cycle progression. It induces cell death by reducing the expression of the antiapoptotic gene Bcl-2 and increasing the expression of the proapoptotic genes Bax, Caspase 3, and Caspase 9. Additionally, the docking analysis and immunoblot investigations verified that GE1-siRNA-CSNPs, which specifically target TNBC cells and suppress miRNA-21, can prevent the effects of miRNA-21 on the proliferation of TNBC cells via controlling EGFR and subsequently inhibiting the PI3K/AKT and ERK1/2 signaling axis. The GE11-siRNA-CSNPs design, which specifically targets TNBC cells, offers a novel approach for the treatment of breast cancer with improved effectiveness. This study suggests that GE11-siRNA-CSNPs could be a promising candidate for further assessment as an additional strategy in the treatment of TNBC. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Functionalized siRNA-chitosan nanoformulations promote triple-negative breast cancer cell death via blocking the miRNA-21/AKT/ERK signaling axis: in-silico and in vitro studies

Abdulmalek,

Saleh,

Shahin

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Cytotoxic potential of Curcuma caesia rhizome extract and derived gold nanoparticles in targeting breast cancer cell lines

Das,

Borah,

Kalita

et al. 2024

Sci Rep

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Among all types of cancer, breast cancer is the most aggressive, as it is responsible for most of the cancer related death of women. Though several medical therapies are available, the scenario of curing such disease is not favorable. Therefore, there is an urgent need to find alternatives to deal with it. The knowledge of ethnopharmacy might give some better solution to mitigate such deadly diseases. Here, we are using the rhizome of Curcuma caesia Roxb. (Black turmeric), as well as gold nanoparticles (GNPs) synthesized with it to check their specific cytotoxic potentiality against breast cancer cell lines. In our study, ethanolic extract was used to evaluate the cytotoxic effect of the rhizome. GNPs were synthesized by using the same extract and characterized by UV-Vis spectroscopy (UV-Vis), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Thermo gravimetric analysis (TGA). The TEM, XRD, FTIR and TGA results revealed the successful synthesis and capping of GNPs. The UV-Vis Spectrum showed a sharp and narrow absorption peak at 550 nm and HRTEM confirmed both the stability and successful synthesis of the nanoparticles. The MTT assay of the crude extract revealed strong cytotoxicity against breast cancer cell lines viz. MCF-7 (ER + ) and MDA MB-231 (Triple Negative Breast Cancer, TNBC) by showing IC 50 values as 15.70 ± 0.029 and 21.57 ± 0.031 μg/mL respectively. For extract mediated GNPs, the IC 50 values were found to be 6.44 ± 0.045 and 5.87 ± 0.031μg/mL respectively in both breast cancer cell lines. As the IC 50 value for GNPs was found to be much lower than that of crude extract, it indicates a higher efficiency of the GNP. However, both the rhizome extract and its mediated GNPs showed more toxicity towards MDA MB-231 (TNBC) cell lines. It was also observed that the GNPs showed more toxicity towards TNBC cell lines compared to the rhizome extract. No toxicity was found in case of other cell lines such as L 929 and HeLa for both crude extract as well as for GNPs. These observations suggests that both the crude rhizome extract and its derived GNPs exhibit selective cytotoxic potential against breast cancer cell lines, which might be exploited for target specific treatment. Moreover, with an understanding of the mechanism behind the GNPs therapeutic efficiency, it can be developed as a personalized therapy to treat such type of cancers.

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Effect of Lantana camara Ethanolic Leaf Extract on Survival and Migration of MDA-MB-231 Triple-Negative Breast Cancer Cell Line

Cited by 2 publications

References 59 publications

Functionalized siRNA-chitosan nanoformulations promote triple-negative breast cancer cell death via blocking the miRNA-21/AKT/ERK signaling axis: in-silico and in vitro studies

Functionalized siRNA-chitosan nanoformulations promote triple-negative breast cancer cell death via blocking the miRNA-21/AKT/ERK signaling axis: in-silico and in vitro studies

Cytotoxic potential of Curcuma caesia rhizome extract and derived gold nanoparticles in targeting breast cancer cell lines

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