Effect of Laser Fluence on a Microarray Droplets Micro-Organisms Cells by LIFT Technique

Haider, Adawiya J.; Haider, Mohammed J.; Majed, Mirvat D.; Mohammed, Ahmed J.; Mansour, Hazim Louis

doi:10.1016/j.egypro.2017.07.078

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…LIFT has been applied for isolation and cultivation of microbial cells as well because of its ability to isolate the bacterial cells without destroying the microenvironment (for example, ejecting the bacteria and the surrounding soil together, but not focusing on single-cell ejection). Haider et al used titanium oxide as the energy absorption layer and studied the effect of laser energy on the viability of yeast and Escherichia coli ( 36 ). Some researchers made use of LIFT’s advantage to isolate and culture the “unculturable” microorganisms from soil in nature ( 37 ) or analyze the soil microbial community ( 38 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology

Peng

Liu

Wang³

et al. 2022

Appl Environ Microbiol

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Single cell isolation and cultivation play an important role in studying physiology, gene expression and functions of microorganisms. A series of single-cell isolation technologies have been developed, among which single-cell ejection technology is one of the most promising. Single cell ejection technology has applied Laser Induced Forward Transfer Technique (LIFT) to isolate bacteria but the viability (or recovery rate) of cells after sorting has not been clarified in the current research progress. In this work, to keep the cells alive as much as possible, we propose a three-layer LIFT system (top layer: 25-nm aluminum film; second layer: 3 μm agar media; third layer: liquid containing bacterial) for the isolation and cultivation of single Gram-negative ( E. coli ), Gram-positive ( Lactobacillus rhamnosus GG, LGG), and eukaryotic microorganisms ( Saccharomyces cerevisiae ). The experiment results showed that the average survival rates for ejected pure single cells were 63% for Saccharomyces cerevisiae , 22% for E. coli DH5α, and 74% for LGG. In addition, we successfully isolated and cultured the GFP expressing E. coli JM109 from the mixture containing complex communities of soil bacteria by fluorescence signal. The average survival rate of E. coli JM109 was demonstrated to be 25.3%. In this study, the isolated and cultured single colonies were further confirmed by colony PCR and sequencing. Such precise sorting and cultivation technique of live single microbial cells could be coupled with other microscopic approaches to isolate single microorganisms with specific functions, revealing their roles in the natural community. Importance We developed a laser induced forward transfer (LIFT) technology to accurately isolate single live microbial cells. The cultivation recovery rates of the ejected single cells were 63% for Saccharomyces cerevisiae , 22% for E. coli DH5α, and 74% for Lactobacillus rhamnosus GG (LGG). Coupled LIFT with fluorescent microscope, we demonstrated that single cells of GFP expressing E. coli JM109 were sorted according to fluorescence signal from a complex community of soil bacteria, and subsequently cultured with 25% cultivation recovery rate. This single cell live sorting technology could isolate single microbes with specific functions, revealing their roles in the natural community.

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Section: Introductionmentioning

confidence: 99%

Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology

Peng

Liu

Wang³

et al. 2022

Appl Environ Microbiol

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“…A good design of EAL can increase the printing resolution, promote productivity, and enhance cell viability. Various materials have been tested to fabricate the absorbing layer, which include Ti [80], titanium oxide [55,81], Au [48], Ag [74], and polyimide [50] as listed in Tab. 2.…”

Section: Laser-absorbing Layer Of An Lab Systemmentioning

confidence: 99%

“…Ag [74] Au [48] Ti [80] Titanium oxide [55,81] Polyimide [50] a stable jet will appear and the bioink can be transferred in an organized way. In this range, the volume of deposited biomaterial linearly depends on the laser pulse energy [70].…”

Section: Eal Materialsmentioning

confidence: 99%

A State‐of‐the‐Art Review of Laser‐Assisted Bioprinting and its Future Research Trends

Dou

Pérez

et al. 2021

ChemBioEng Reviews

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Bioprinting is an additive manufacturing technology with great potential in medical applications. Among available bioprinting techniques, laser-assisted bioprinting (LAB) is a promising technique due to its high resolution, high cell viability, and the capability to deposit high-viscousity bioink. These characteristics allow the LAB technology to control cells precisely to reconstruct living organs. Recent developments of LAB technologies are reviewed in this paper, covering various designs of LAB printers, re-search progresses in energy-absorbing layer (EAL), the physical phenomenon that triggers the printing process in terms of bubble formation and jet development, printing process parameters, and major factors related to the post-printing cell viability. The latest studies on LAB technologies are highlighted, expounding their advantages and disadvantages, and some potential applications are presented. The potential technical challenges and future research trends for LAB technologies are also discussed.

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“…; Haider et al . ). Recently, effectiveness of this technique for bacterial isolation from different complex substrates was demonstrated (Ringeisen et al .…”

Section: Introductionmentioning

confidence: 97%

Laser printing of microbial systems: effect of absorbing metal film

Cheptsov

Churbanova

Yusupov

et al. 2018

Lett Appl Microbiol

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Recently, it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of micro-organisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of this study was to investigate the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness of laser printing of micro-organisms to improve LEMS and LIFT techniques. It was shown that application of Ti and Cr absorbing layers activates bacterial growth after laser printing and is significantly more effective in comparison to Au films, which actually show a suppressing effect on bacterial cells. Results of this study can be applied for LEMS and LIFT protocols for improving bacterial isolation and microbial growth. SIGNIFICANCE AND IMPACT OF THE STUDY: Laser-induced forward transfer technique (LIFT) is currently used for printing of micro-organisms and in biosensor techniques, for single-cell isolation, and for culturing of micro-organisms from complex substrates. We have studied the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness laser printing of micro-organisms. It was shown that application of Ti and Cr absorbing layers activates bacterial growth and is more effective in LIFT compared to Au films, which actually have a suppressive effect on bacteria cells. The results can improve LIFT protocols for bacteria isolation and culturing of microbial systems.

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Effect of Laser Fluence on a Microarray Droplets Micro-Organisms Cells by LIFT Technique

Cited by 11 publications

References 22 publications

Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology

Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology

A State‐of‐the‐Art Review of Laser‐Assisted Bioprinting and its Future Research Trends

Laser printing of microbial systems: effect of absorbing metal film

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