The bottom-locking joint is widely used in the aerospace manufacturing eld as an imperative connection structure. The laser welding experiment for the Ti-6Al-4V titanium alloy bottom-locking joint is carried out in the present paper. Meanwhile, porosity in the weld seam including the process porosity and metallurgical porosity is investigated. Furthermore, it is novel that the distribution, element characteristics, and formation mechanism of process porosities and metallurgical porosities in the bottom-locking joint are comprehensively analyzed based on the porosity morphology, microstructure, and element analysis of the resultant bottom-locking joints. The process porosity, which mainly appeared in the weld seam centre, is formed by the combined effect of two mechanisms which closely related to the dynamic behaviour of the molten pool and the stability of the keyhole. Besides, a large number of micropores with a diameter of 2-5 µm are observed on the surface of the process porosity, whose formation is related to the evaporation of elements. Moreover, the tensile stress generated during the solidi cation and shrinkage of the molten metal is the main factor leading to microcracks in the micropores. The nucleation characteristics of bubbles cause metallurgical porosities to be mainly distributed at the bottom of the weld seam.