The non-contact labyrinth seal structure of a high-speed gearbox was taken as the research object, a non-contact labyrinth seal structure under negative pressure intervention was designed, and its rationality was verified by simulation analysis. An airfoil-shaped acquisition device for collecting negative pressure was designed, and its rationality was analyzed by simulation. According to the design, a test platform for non-contact labyrinth seal under negative pressure intervention was built to measure the pressure data of each sealing chamber of the non-contact labyrinth seal under different working conditions. The results showed that it was effective to balance the internal and external pressure of the non-contact labyrinth seal by using the negative pressure collected by the airfoil-shaped device in the complex flow field of the high-speed gearbox. It also showed that the non-contact labyrinth seal structure under negative pressure intervention designed in this paper is an effective way to solve the leakage problem of the non-contact labyrinth seal used in high-speed gearbox.