Effect of Lead on Bone Development and Bone Mass: A Morphometric, Densitometric, and Histomorphometric Study in Growing Rats

Escribano, Arancha; Revilla, M.; Hernández, E. R.; Seco, C.; González-Riola, J.; Villa, L. F.; Rico, H.

doi:10.1007/s002239900214

Cited by 67 publications

(44 citation statements)

References 24 publications

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“…Because of the influence of weight on bone mass [11], femur BMC was corrected for the animals final body weight (femur BMC/FW mg/g). As in our earlier studies [11][12][13][14], we have relied on DXA (Norland XR-26, Norland Co., Fort Atkinson, Wisconsin, USA). Our coefficients of variation (CV) for the current measurements, determined by six separate measurements done on three rat femurs and 5th vertebras at intervals of 3-4 days were 0.8% and 0.7%, respectively.…”

Section: Morphometric and Densitometric Studiesmentioning

confidence: 99%

Effect of Silicon Supplement on Osteopenia Induced by Ovariectomy in Rats

Rico

Gallego-Lago

Hernández

et al. 2000

Calcified Tissue International

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The effect of silicon (Si) supplement on preventing bone mass loss induced by ovariectomy (OVX) in rats was investigated. Three groups of 15, 100-day-old female Wistar rats each, with a mean initial weight of approximately 260 g per animal, were selected for the present study. One of the experimental group consisting of 15 OVX rats was fed a diet supplemented with 500 mg of Si per kg of feed (Si + OVX). The other two groups consisting of 15 OVX and 15 sham-OVX rats did not receive these supplements. Morphometric (weight and length) and densitometric studies with dual-energy X-ray absorptiometry were performed on the whole femur and 5th lumbar vertebra of each animal 30 days after the experiment. The Si + OVX rats did not show a loss of bone mass induced by OVX at axial level (5th lumbar vertebra) or periphery (femur). Nonetheless, a significant increase (ANOVA with Bonferroni/Dunn post hocs test) of longitudinal development of the femur (P < 0.0001) was patent. These results, obtained through the measurements of axial and peripheral bones, warrant closer scrutiny in connection with the Si inhibitory effect on bone mass loss as well as the stimulatory effect on bone formation. Both actions, namely, inhibition of resorption and stimulation of formation, infer that Si may have a potential therapeutic application in the treatment of involutive osteoporosis.

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Section: Morphometric and Densitometric Studiesmentioning

confidence: 99%

Effect of Silicon Supplement on Osteopenia Induced by Ovariectomy in Rats

Rico

Gallego-Lago

Hernández

et al. 2000

Calcified Tissue International

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“…After bone densitometry, the femurs were processed using techniques described elsewhere [16][17][18] for the histomorphometric study of nondecalcified bone. The bones were sectioned longitudinally into 7-micra slices using a Microm microtome (Microm, Heidelberg, Germany) and were stained with Goldner stain.…”

Section: Histomorphometric Studiesmentioning

confidence: 99%

Effects of Promethazine on Bone Mass and on Bone Remodeling in Ovariectomized Rats: A Morphometric, Densitometric, and Histomorphometric Experimental Study

et al. 1999

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The effect of promethazine on bone is debated. We studied the effect of promethazine on bone and the mechanism of action involved by densitometric and histomorphometric measurements in female Wistar rats (100 days old, mean weight 25 +/- 20 g). A control group of 15 rats was not manipulated. An experimental group of 15 rats were ovariectomized (OVX) at 100 days of life and fed a diet supplemented with 4.8 mg/kg promethazine hydrochloride (OVX + Prom). The group that underwent OVX and a group of 15 rats that underwent sham ovariectomy (Sham-OVX) were not treated with promethazine. After 30 days, all the rats were killed. Their femur and 5th lumbar vertebra were dissected and cleaned of soft tissue. Femoral length and vertebral height were measured with a caliper and bones were weighed on a precision balance. The bone mineral content (BMC) and bone mineral density (BMD) of the whole right femurs and 5th lumbar vertebras were measured by dual-energy X-ray absorptiometry (DXA). Trabecular bone volume (Cn-BV-TV%), trabecular number (Tb-N mm(-1)), trabecular thickness (Tb-Th microm), and trabecular separation (Tb-Sp microm) were measured in the femurs by histomorphometric study of nondecalcified bone. Our results showed that promethazine significantly inhibited postovariectomy loss of bone mass (P < 0. 0001) by significantly reducing bone resorption, as shown by the smaller trabecular spaces observed in the treated OVX rats (P < 0. 0001).

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“…5 Diseases or states with increased bone turnover, such as osteoporosis, pregnancy, hyperthyroidism and hyperparathyroidism, are associated with increased mobilization of Pb from the skeleton. 6 -8 It has been reported that Pb plays a role in osteopenia 9,10 and inhibits fracture healing. 11 Moreover, intra-articular lead has…”

Section: Introductionmentioning

confidence: 99%

Determination of the elemental distribution in human joint bones by SR micro XRF

et al. 2007

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In humans the toxic trace element lead (Pb) mainly accumulates in the skeletal tissue where it resides for a long period. Although Pb is known to play a role in bone and cartilage diseases, the distribution of Pb in these tissues is mostly unknown. Therefore synchrotron‐radiation‐induced micro x‐ray fluorescence analysis (SR µ‐XRF) in various geometries was used to determine the distributions of Pb, Ca, Zn and Sr at the cartilage‐bone interface in human femoral heads and patellae. SR µ‐XRF results were matched with backscattered electron (BE) images providing information on structural features of the tissue. Conventional SR µ‐XRF at HASYLAB beamline L showed that Pb mostly accumulates in a zone of some micrometers around the transition between non‐calcified and calcified cartilage. However, the relatively large sample thickness did not allow more precise conclusions. Exploiting the 3D capabilities of confocal SR µ‐XRF and SR microfluorescence tomography at ANKA Fluo‐Topo beamline and HASYLAB beamline L, the spatial resolution could be improved and a highly specific accumulation of Pb at the tidemark, the calcification front between non‐calcified and calcified cartilage, was detected. It was found that Pb and Zn accumulation coincide at the tidemark. Since the tidemark plays an important role in osteoarthritis, the results may strengthen other authors' conclusions that Pb is associated with this joint disease. Although offering less spatial resolution when compared to micro‐tomography, confocal SR µ‐XRF is best suited for such studies. It facilitates the comparison of element maps with other imaging techniques like BE imaging. Copyright © 2007 John Wiley & Sons, Ltd.

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Effect of Lead on Bone Development and Bone Mass: A Morphometric, Densitometric, and Histomorphometric Study in Growing Rats

Cited by 67 publications

References 24 publications

Effect of Silicon Supplement on Osteopenia Induced by Ovariectomy in Rats

Effect of Silicon Supplement on Osteopenia Induced by Ovariectomy in Rats

Effects of Promethazine on Bone Mass and on Bone Remodeling in Ovariectomized Rats: A Morphometric, Densitometric, and Histomorphometric Experimental Study

Determination of the elemental distribution in human joint bones by SR micro XRF

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