Effect of lead on proliferation and neural differentiation of mouse bone marrow-mesenchymal stem cells

Kermani, Shabnam; Karbalaie, Khadijeh; Madani, Seyed Hossein; Jahangirnejad, Ali Akbar; Eslaminejad, Mohamadreza Baghaban; Nasr-Esfahani, Mohammad Hossein; Baharvand, Hossein

doi:10.1016/j.tiv.2008.02.009

Cited by 21 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our hands, however, 0.1 µM Pb reduced cell numbers after CA disaggregation without inducing concomitant cell death directly and without activating caspase-3. These results are remarkably similar to those described for low-concentration, long-term treatment of neural stem cells derived from E15 rat cortex, striatum, and ventral mesencephalon, and mouse bone marrow-mesenchymal stem cells, which show that cell proliferation is slightly altered at low Pb concentration and considerably affected at high concentration [ 54 , 55 ]. We tested treatment of ESC with different Pb concentrations in the range of 0.01 to 0.1 µM for 10 days and we did not observe any reduction of cell proliferation ( Figure S2 ).…”

Section: Discussionsupporting

confidence: 86%

Lead Induces Similar Gene Expression Changes in Brains of Gestationally Exposed Adult Mice and in Neurons Differentiated from Mouse Embryonic Stem Cells

et al. 2013

View full text Add to dashboard Cite

Exposure to environmental toxicants during embryonic life causes changes in the expression of developmental genes that may last for a lifetime and adversely affect the exposed individual. Developmental exposure to lead (Pb), an ubiquitous environmental contaminant, causes deficits in cognitive functions and IQ, behavioral effects, and attention deficit hyperactivity disorder (ADHD). Long-term effects observed after early life exposure to Pb include reduction of gray matter, alteration of myelin structure, and increment of criminal behavior in adults. Despite growing research interest, the molecular mechanisms responsible for the effects of lead in the central nervous system are still largely unknown. To study the molecular changes due to Pb exposure during neurodevelopment, we exposed mice to Pb in utero and examined the expression of neural markers, neurotrophins, transcription factors and glutamate-related genes in hippocampus, cortex, and thalamus at postnatal day 60. We found that hippocampus was the area where gene expression changes due to Pb exposure were more pronounced. To recapitulate gestational Pb exposure in vitro, we differentiated mouse embryonic stem cells (ESC) into neurons and treated ESC-derived neurons with Pb for the length of the differentiation process. These neurons expressed the characteristic neuronal markers Tubb3, Syp, Gap43, Hud, Ngn1, Vglut1 (a marker of glutamatergic neurons), and all the glutamate receptor subunits, but not the glial marker Gafp. Importantly, several of the changes observed in Pb-exposed mouse brains in vivo were also observed in Pb-treated ESC-derived neurons, including those affecting expression of Ngn1, Bdnf exon IV, Grin1, Grin2D, Grik5, Gria4, and Grm6. We conclude that our ESC-derived model of toxicant exposure during neural differentiation promises to be a useful model to analyze mechanisms of neurotoxicity induced by Pb and other environmental agents.

show abstract

Section: Discussionsupporting

confidence: 86%

Lead Induces Similar Gene Expression Changes in Brains of Gestationally Exposed Adult Mice and in Neurons Differentiated from Mouse Embryonic Stem Cells

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Analysis on the expression of other specific neuron markers, such as MAP-2, 35 , 36 has been used for neural confirmation. These mature-specific markers played a role in the stability of axons and neuronal cell bodies through the differentiation process; 37 they were absent or had low expressions in the early stage of neural differentiation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells

Mohammad

Al-Shammari

Al-Juboory

et al. 2016

SCCAA

View full text Add to dashboard Cite

The in vitro isolation, identification, differentiation, and neurogenesis characterization of the sources of mesenchymal stem cells (MSCs) were investigated to produce two types of cells in culture: neural cells and neural stem cells (NSCs). These types of stem cells were used as successful sources for the further treatment of central nervous system defects and injuries. The mouse bone marrow MSCs were used as the source of the stem cells in this study. β-Mercaptoethanol (BME) was used as the main inducer of the neurogenesis pathway to induce neural cells and to identify NSCs. Three types of neural markers were used: nestin as the immaturation stage marker, neurofilament light chain as the early neural marker, and microtubule-associated protein 2 as the maturation marker through different time intervals in the neurogenesis process starting from the MSCs, (as undifferentiated cells), NSCs, production stages, and toward neuron cells (as differentiated cells). The results of different exposure times to BME of the neural markers analysis done by immunocytochemistry and real time-polymerase chain reaction helped us to identify the exact timing for the neural stemness state. The results showed that the best exposure time that may be used for the production of NSCs was 6 hours. The best maintenance media for NSCs were also identified. Furthermore, we optimized exposure to BME with different times and concentrations, which could be an interesting way to modulate specific neuronal differentiation and obtain autologous neuronal phenotypes. This study was able to characterize NSCs in culture under differentiation for neurogenesis in the pathway of the neural differentiation process by studying the expressed neural genes and the ability to maintain these NSCs in culture for further differentiation in thousands of functional neurons for the treatment of brain and spinal cord injuries and defects.

show abstract

“…Similar results have been reported by other groups. [58][59][60][61] Figure 4 showed that MSCs proliferated on aligned and random PCL nanofibers in the presence of nerve induction medium expressed a higher Map-2 gene compared with that on tissue culture plate. Our results showed that upregulation of Map-2 was observed on MSCs proliferated in 3D scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Controlled surface morphology and hydrophilicity ofpolycaprolactonetoward selective differentiation of mesenchymal stem cells to neural like cells

Jahani

Jalilian

et al. 2014

J Biomedical Materials Res

View full text Add to dashboard Cite

Differentiation of mesenchymal stem cells (MSCs) into neuron cells has great potential in therapy of damaged nerve tissue. It has been shown that three-dimensional biomaterials have great ability to up regulate the expression of neuronal proteins. In this study, O2 plasma technology was used to enhance hydrophilicity of poly (ε-caprolactone) (PCL) toward selective differentiation of MSCs into neural cells. Random and aligned PCL nanofibers scaffolds were fabricated by electrospinning method and their physicochemical and mechanical properties were carried out by scanning electron microscope (SEM), contact angle, and tensile measurements. Contact angle studies of PCL and plasma treated PCL (p-PCL) nanofibers revealed significant change on the surface properties PCL nanofibers from the view point of hydrophilicity. Physiochemical studies revealed that p-PCL nanofibers were extremely hydrophilic compared with untreated PCL nanofibers which were highly hydrophobic and nonabsorbent to water. Differentiation of MSCs were carried out by inducing growth factors including basic fibroblast growth factor, nerve growth factor, and brain derived growth factor, NT3, 3-isobutyl-1-methylxanthine (IBMX) in Dulbecco's modified Eagle's medium/F12 media. Differentiated MSCs on nanofibrous scaffold were examined by immunofluorescence assay and was found to express the neuronal proteins; β-tubulin III and Map2, on day 15 after cell culture. The real-time polymerase chain reaction (RT-PCR) analysis showed that p-PCL nanofibrous scaffold could upregulate expression of Map-2 and downregulate expression of Nestin genes in nerve cells differentiated from MSCs. This study indicates that mesenchymal stem cell cultured on nanofibrous scaffold have potential differentiation to neuronal cells on and could apply in nerve tissue repair.

show abstract

Effect of lead on proliferation and neural differentiation of mouse bone marrow-mesenchymal stem cells

Cited by 21 publications

References 17 publications

Lead Induces Similar Gene Expression Changes in Brains of Gestationally Exposed Adult Mice and in Neurons Differentiated from Mouse Embryonic Stem Cells

Lead Induces Similar Gene Expression Changes in Brains of Gestationally Exposed Adult Mice and in Neurons Differentiated from Mouse Embryonic Stem Cells

Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells

Controlled surface morphology and hydrophilicity ofpolycaprolactonetoward selective differentiation of mesenchymal stem cells to neural like cells

Contact Info

Product

Resources

About