Objective: To investigate the effect of performing three different toe touch (TT) task condition on the activities of four different muscles using surface electromyography (sEMG) in healthy young adults. Design: Cross-sectional study. Methods: A total of 20 healthy young adults (6 males, 14 females) voluntarily participated in this study. All subject randomly performed three different TT task conditions as follows: general toe-touch (GTT) task, one side toe touch (TT) task during weight bearing, and one side foward toe touch (FTT) task during weight bearing. The muscle activities of erector spinae (ES), gluteus maximus (GM), hamstring (HAM), tibialis anterior (TA) muscles during the TT task were measured using sEMG. Subject performed each of the three conditions three time in random order and mean values were obtained. Results: With the trunk flexion period, the TT and FTT showed significantly greater muscle activity in the GM, HAM and TA compared to the GTT task (p<0.05). The TT position showed significantly greater HAM muscle activity than the GTT position. The dominant and nondominant ES muscle activity was significantly greater in the FTT compared to the GTT position (p<0.05). The dominant GM, HAM, and TA was significantly greater in the TT and FTT compared to the GTT position (p<0.05). Although the dominant ES was significantly greater in the TT and FTT compared to the GTT position (p<0.05), the dominant GM muscle activity was signifcantly greater in the TT compared with the GTT position (p<0.05). Conclusions: These findings may be applicable within the clinical field for selective trunk and lower extremity muscle activation and basic biomechanics purpose.