Effect of leptin on in vitro development of ovine preantral ovarian follicles

Kamalamma, P.; Kona, S.S.R.; Chakravarthi, V. Praveen; Kumar, Arvind; Punyakumari, B.; Rao, V. R.

doi:10.1016/j.theriogenology.2015.09.045

Cited by 28 publications

(34 citation statements)

References 48 publications

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“…The culture procedure was found to be optimum in previous studies in the laboratory (Hemamalini et al., ; Tamilmani et al. ; Arunakumari et al., , ; Kamalamma et al., ). The day on which the PFs were placed in the culture was designated as day 0 and the subsequent days as day 1, 2 and so on.…”

Section: Methodsmentioning

confidence: 92%

“…These culture dishes were incubated at 39°C under humidified atmosphere in 5% CO 2 in air for up to 6 days. The culture procedure was found to be optimum in previous studies in the laboratory (Hemamalini et al, 2003;Tamilmani et al 2005;Arunakumari et al, 2007Arunakumari et al, , 2010Kamalamma et al, 2016). The day on which the PFs were placed in the culture was designated as day 0 and the subsequent days as day 1, 2 and so on.…”

Section: Selection and Culture Of The Preantral Folliclesmentioning

confidence: 99%

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Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Srividya

Chakravarthi

Kona

et al. 2017

Reprod Domestic Animals

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Expression of Kit ligand (KL) and insulin-like growth factor binding protein (IGFBP3) genes was studied at different in vivo and corresponding in vitro stages of development of the ovarian follicles in sheep. The expression of both KL and IGFBP3 was significantly higher in the primordial follicles relative to any other stage of development. Compared to the other stages, the KL expression in the cumulus cells from in vivo grown large antral follicles and that of IGFBP3 in COCs' isolated from large antral follicles matured in vitro for 24 hr were significantly higher. In the oocytes from in vivo grown ovarian follicles, the expression of KL was the same at all the stages of development. Insulin-like growth factor binding protein 3 expression was also the same in the oocytes at all the stages of the development except for a significantly lower expression in those from antral follicles. The expression of KL in the cumulus cells decreased significantly in the in vitro grown early antral follicles but did not change further as the development progressed. The expression of IGFBP3 in the cumulus cells from in vitro grown ovarian follicles appeared to increase as the development progressed although the increase was not significant between any two consecutive stages of development. In the oocytes in in vitro grown ovarian follicles, the expression levels of KL and IGFBP3 genes did not change with development. It is concluded that (i) KL and IGFBP3 genes follow specific patterns of expression during ovarian folliculogenesis and (ii) in vitro culture of preantral follicles compromises the development potential through alterations in the stage-specific patterns of expression of these and other developmentally important genes.

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Section: Methodsmentioning

confidence: 92%

Section: Selection and Culture Of The Preantral Folliclesmentioning

confidence: 99%

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Srividya

Chakravarthi

Kona

et al. 2017

Reprod Domestic Animals

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“…The disturbs of the TH in murine causes an imbalance in both redox homeostasis (10) and estradiol levels leading to follicular apoptosis (11). To better understand about the effects of TH at the cellular level, in vitro experiments have been performed either using T3 (12-14) or T4 (15-19). The use of T3 and/or T4 in association to other hormones enhance survival and development of secondary follicles in murine (12, 14), and ovine (17), as well as bovine embryo development (13, 16).…”

Section: Introductionmentioning

confidence: 99%

The subtle balance of insulin and thyroxine on survival and development of primordial follicles culturedin vitroenclosed in ovarian tissue

Paes

Lima

Ferreira

et al. 2019

Preprint

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23Thyroid hormones have presented a positive hormonal interaction on follicular 24 development of secondary follicles and oocytes from antral follicles; however, the effect 25 of thyroid hormones on primordial follicles is unclear. Here we investigated the in vitro 26 effects of combined insulin and thyroxine on caprine primordial follicle survival and 27 development. Ovarian tissues were cultured for 1 or 7 days using 10 ng/ml (low) or 10 28 µg/ml (high) insulin in the absence or presence of thyroxine at 0.5, 1 or 2 µg/ml. 29Thereafter, follicular survival and development, gene expression related to apoptosis 30 (Bcl2/Bax), insulin and thyroid receptors, and estradiol and reactive oxygen species 31 production were evaluated. In low-insulin conditions, supplementation with 2 µg/ml 32 thyroxine maintained follicular survival similar to non-cultured control, while 0.5 µg/ml 33 thyroxine enhanced the survival (P<0.05) in comparison to thyroxine-free treatment. Only 34 treatments containing low-insulin and thyroxine at 0.5 or 2 µg/ml increased (P<0.05) 35 reactive oxygen species production from day 1 to day 7. Contrarily to high-insulin 36 containing medium, the presence of thyroxine in low-insulin medium yielded higher 37 stromal cell density (P<0.05). There were higher (P<0.05) estradiol production and 38 Bcl2/Bax ratio in low-insulin versus high-insulin treatments on day 1 and 7, respectively. 39High levels of both insulin and thyroxine showed better follicular development (P<0.05), 40 yielding great follicle and oocyte diameter. Finally, the high-insulin level led to insulin 41 and thyroid receptors expression reduction as compared to non-cultured control. In 42 conclusion, the combination of low concentrations of insulin and thyroxine better 43 maintained follicle survival, while high levels ensured better follicular development. 44 45 3 46 65 20). Nevertheless, even though few in vitro studies reported the effects of TH on the 66 development of late preantral follicle categories to the best of our knowledge of its in 67 vitro role on primordial follicle survival and development remains unknown. 68 Among the various hormones that act on folliculogenesis, insulin has an important 69 role in follicle development due to its action on cell survival and proliferation (21). Our 4 70team has shown that insulin effects could be influenced by follicular category (22, 23), as 71 well as its interactions with other substances (24)(25)(26). For instance, the use of insulin at 72 10 ng/ml during in vitro culture of either primordial (22), secondary or tertiary follicles 73 (23) yielded greater oocyte growth than using 10 µg/ml insulin. On the other hand, 74 secondary follicles cultured in vitro with insulin at 10 µg/ml in association to FSH (24) 75 and GH (25), or FSH + VEGF (26) enhance meiosis resumption in goat oocytes. 76Although the hormonal network is a complex process, the balance of insulin and 77 thyroxine concentration on in vitro primordial follicles development is unclear. 78Therefore, the novelty of the...

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“…In sheep, some results are promising, for instance, the growth and antrum formation have been successfully achieved during in vitro culture of ovine secondary follicles (SF) (Barberino et al, ; Kamalamma et al, ). Moreover, our team has also reported that it is possible to produce parthenotes embryos from large SF (>200 µm), grown in vitro (Luz et al, ).…”

Section: Introductionmentioning

confidence: 99%

Early ovine preantral follicles have a potential to grow until antral stage in two‐step culture system in the presence of aqueous extract of Justicia insularis

Mbemya

Sá

Guerreiro

et al. 2019

Reprod Domestic Animals

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The objective of this study was to determine whether preantral follicles cultured in vitro for 7 days within ovine ovarian cortical strips could be isolated at the secondary follicles (SF) and grown until antral stage during an additional 6 days period of in vitro culture in the presence of aqueous extract of Justicia insularis. Fresh ovarian fragments from 16 adult sheep were fixed for histological analysis (Control 1) or in vitro cultured individually in α‐MEM+ supplemented with 0.3 mg/ml J. insularis (Step 1) for 7 days. Part of the fragments then were fixed for histological analysis (in vitro culture group). Remaining fragments were exposed stepwise to increasing trehalose concentrations before immediate isolation of SF and viability assessment (Control 2) or after 6 days of culture in α‐MEM++ supplemented with 0.3 mg/ml J. insularis (Step 2). In Step 1, percentage of follicular activation was 80%. In Step 2, a significant increase (p < 0.05) in follicular diameter and antrum formation within 6 days in vitro culture of isolated follicles was achieved. The total antioxidant capacity from both steps significantly increase (p < 0.05) from day 2 to day 6. Confocal analysis of oocytes showed 57.14% oocytes with homogeneous distribution and 42.86% with peri‐cortical distribution. In conclusion, SF can be successfully isolated from sheep ovarian cortex after 7 days of culture and are capable of surviving and forming an antral cavity if cultured in vitro for an additional 6 days in the presence of 0.3 mg/ml J. insularis.

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Effect of leptin on in vitro development of ovine preantral ovarian follicles

Cited by 28 publications

References 48 publications

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

The subtle balance of insulin and thyroxine on survival and development of primordial follicles culturedin vitroenclosed in ovarian tissue

Early ovine preantral follicles have a potential to grow until antral stage in two‐step culture system in the presence of aqueous extract of Justicia insularis

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