Effect of lifestyle exposures on sperm aneuploidy

Robbins, Wendie A.; Elashoff, David; Lin, Xiao; Jia, Juan; Li, N.; Wu, Guoping; Wei, Fusheng

doi:10.1159/000086914

Cited by 78 publications

(44 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A significant increase in sperm disomy rates was observed among smoking patients compared with non smoking patients [16,29], and in XY hyperhaploidy in sperm of alcohol drinkers compared with non-drinkers [29]. However our results did not show any correlation between these two parameters and high risk of increased sperm aneuploidies rates.…”

Section: Discussioncontrasting

confidence: 87%

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Mougou-Zerelli

Brahem

Kammoun

et al. 2011

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To evaluate the frequency of sperm nuclei disomy for chromosomes 8, X, and Y in patients with severe nonobstructive oligozoospermia and to assess possible correlations between sperm nuclei aneuploidy and semen parameters or a particular clinical phenotype. Materials and methods The sperm aneuploidy rate for chromosomes X, Y, and 8 were assessed in 16 infertile men with severe non-obstructive oligozoospermia and 7 healthy men with normal semen parameters. The frequency of sperm aneuploidy was compared between several patients groups according to their clinical and biological factors. Results The total rate of chromosomally abnormal spermatozoa was significantly higher in patients with severe oligozoospermia compared to control group (P<0.001). A significant relationship was found between the age of patients, sperm concentration, and morphology and the mean rate of sex chromosomes disomy. In addition to the low sperm count (<5×10 6 /ml), an elevated FSH level and an exposed to an elevated temperature are two major predictive factors leading to the production of higher numbers of chromosomally abnormal gametes. Conclusion Patients with severe oligozoospermia, who are potential candidates for assisted reproduction technology, presented a high level of sex numerical chromosome abnormalities, and consequently are at high risk of chromosome abnormalities in their offspring.

show abstract

Section: Discussioncontrasting

confidence: 87%

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Mougou-Zerelli

Brahem

Kammoun

et al. 2011

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Wyrobek summarized the human studies that have applied sperm FISH technologies to assess the effects of chemotherapy [Wyrobek et al, 2005b], occupational exposures, and lifestyle factors [Robbins et al, 2005] on the incidence of sperm aneuploidy. He observed a 5-fold variation in baseline frequencies of sperm aneuploidy among healthy donors; however, he also noted that aneuploidy frequencies may remain stably elevated for years within the same donor.…”

Section: Sessions 3/4: New Approaches For Detecting Heritable Mutationsmentioning

confidence: 99%

Assessing human germ‐cell mutagenesis in the Postgenome Era: A celebration of the legacy of William Lawson (Bill) Russell

Wyrobek

Mulvihill

Wassom

et al. 2007

Environ and Mol Mutagen

View full text Add to dashboard Cite

Birth defects, de novo genetic diseases, and chromosomal abnormality syndromes occur in ~5% of all live births, and affected children suffer from a broad range of lifelong health consequences. Despite the social and medical impact of these defects, and the 8 decades of research in animal systems that have identified numerous germ-cell mutagens, no human germ-cell mutagen has been confirmed to date. There is now a growing consensus that the inability to detect human germ-cell mutagens is due to technological limitations in the detection of random mutations rather than biological differences between animal and human susceptibility. A multidisciplinary workshop responding to this challenge convened at The Jackson Laboratory in Bar Harbor, Maine. The purpose of the workshop was to assess the applicability of an emerging repertoire of genomic technologies to studies of human germ-cell mutagenesis. Workshop participants recommended large-scale human germ-cell mutation studies be conducted using samples from donors with high-dose exposures, such as cancer survivors. Within this high-risk cohort, parents and children could be evaluated for heritable changes in (a) DNA sequence and chromosomal structure, (b) repeat sequences and minisatellites, and (c) global gene expression profiles and pathways. Participants also advocated the establishment of a bio-bank of human tissue samples from donors with well-characterized exposure, including medical and reproductive histories. This mutational resource could support large-scale, multipleendpoint studies. Additional studies could involve the examination of transgenerational effects NIH Public AccessAuthor Manuscript Environ Mol Mutagen. Author manuscript; available in PMC 2007 November 8. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript associated with changes in imprinting and methylation patterns, nucleotide repeats, and mitochondrial DNA mutations. The further development of animal models and the integration of these with human studies are necessary to provide molecular insights into the mechanisms of germcell mutations and to identify prevention strategies. Furthermore, scientific specialty groups should be convened to review and prioritize the evidence for germ-cell mutagenicity from common environmental, occupational, medical, and lifestyle exposures. Workshop attendees agreed on the need for a full-scale assault to address key fundamental questions in human germ-cell environmental mutagenesis. These include, but are not limited to, the following: Do human germ-cell mutagens exist? What are the risks to future generations? Are some parents at higher risk than others for acquiring and transmitting germ-cell mutations? Obtaining answers to these, and other critical questions, will require strong support from relevant funding agencies, in addition to the engagement of scientists outside the fields of genomics and germ-cell mutagenesis.

show abstract

“…It has been well documented that severe male infer tility correlates with a significant increase in sperm aneuploidy; in addition, other factors such as paternal age, chemotherapy treatment, smoking and alcohol have also been associated with increased sperm aneuploidy [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Given the significant increased risk of sperm aneuploidy in infertile men, concerns have been raised regarding whether these individuals are at a higher risk of giving rise to aneuploid pregnancies when treated by intracytoplasmic sperm injection (ICSI) [17].…”

Section: Introductionmentioning

confidence: 99%

Scoring of sperm chromosomal abnormalities by manual and automated approaches: qualitative and quantitative comparisons

Tempest¹,

Cheng²,

Gillott³

et al. 2009

Asian J Androl

View full text Add to dashboard Cite

It is now well known that levels of sperm disomy correlate to levels of infertility (as well as other factors). The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sperm injection (ICSI) has become a hotly debated issue in assisted reproduction; however, there remain barriers to the practical implementation of offering sperm disomy screening in a clinical setting. The major barrier is the operator time taken to analyze a statistically meaningful (sufficient) number of cells. The introduction of automated 'spot counting' software-hardware combinations presents a potential solution to this problem. In this preliminary validation study, we analyzed 10 patients, both manually and using a commercially available spot counter. Results show a statistically significant correlation between both approaches for scoring of sperm disomy, but no correlation is found when scoring for diploid sperm. The most likely explanation for the latter is an apparent overscoring of two closely associated sperm heads as a single diploid cell. These results, and similar further studies that will ensue, help to inform cost-benefit analyses that individual clinics need to carry out in order to decide whether to adopt sperm aneuploidy screening as a routine tool for the assessment of sperm from men requiring ICSI treatment.

show abstract

Effect of lifestyle exposures on sperm aneuploidy

Cited by 78 publications

References 95 publications

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Assessing human germ‐cell mutagenesis in the Postgenome Era: A celebration of the legacy of William Lawson (Bill) Russell

Scoring of sperm chromosomal abnormalities by manual and automated approaches: qualitative and quantitative comparisons

Contact Info

Product

Resources

About