Effect of light and benzyladenine on dark‐treated growing rice leaves (Oryza sativa). I. Changes in chlorophyll content and catalase activity

Abstract

1985. Effect of light and benzyladenine on dark-treated growing rice leaves (Oryza saiivti). I. Changes in chlorophyll content and catalase activity. -Physiol. Plant. 63: 79-86.Light-and benzyladeuine-indiiced reversal of the changes in chlorophyll content and catalase activity were studied in the attached first leaf of Oryza sativa L. cv. Bala, kept in darkness for different periods before maturation. Dark treatment caused a decrease in chlorophyll content and catalase activity at all times. Light treatment of dark-incubated seedlings at different periods before maturation reversed the dark-induced effect on chlorophyll content, catalase activity and dry weight and also caused a further rise in chlorophyll content compared to initial values. In darkness, the application of benzyladenine replaced the iight effect in maintaining catalase activity. Chlorophyll content was also maintained by initially applied benzyladenine. Benzyladenine did not promote the photoinduced maintenance and increase in chlorophyll content and catalase activity at any time. Treatment with hydrogen peroxide, glycolate and amizol resulted in an accelerated chlorophyll breakdown and had varied effects on catalase levels. Chlorophyll decrease due to peroxide accumulation was to some extent reversible by benzyladenine, but the hormone had no effect on the peroxide-induced decrease in catalase activity. Development of catalase is light dependent. Benzyladenine stabilises the enzyme but has no effect on its synthesis.

Impact of some phytohormones, metabolic inhibitor and agrochemicals on senescing paddy leaves and its relevance to productivity

“…This concurs similar findings reported in detached rice leaves (Kar and Mishra, 1976;Patra et al, 1979;Reddy et al, 1985), tobacco (Parish, 1968) and oat (Birecka et al, 1979;Kar and Feieraband, 1984). There are also reports of suppression of peroxidase activity by growth regulators in barley leaves (Sharma and Biswal, 1976) and pea stem sections (Henry and Jordan, 1977).…”

“…The information so far available indicated that the excised senescencing leaves incubated in dark exhibited a decreased catalase activity in rice leaves (Kar and Mishra, 1976;Patro et al, 1978), ragi leaves (Reddy et al, 1985). Anagalli leaves (Trippi et al, 1980 …”

Comparative physiological analyses and the genetic basis reveal heat stress responses mechanism among different Betula luminifera populations

“…The assay principle was based on catalase's reaction to breakdown H 2 O 2 , which absorbs maximum at 405 nm. Spectrophotometrically, the POD activity was determined at (Reddy et al, 1985). Purpurogallin, which has a wavelength of 420 nm, is produced in the assay by the reaction of peroxidase and pyrogallol.…”