Effect of light emitting diode photobiomodulation on murine macrophage function after Bothrops envenomation

Reis, Valdison Pereira dos; Rego, Cristina Matiele Alves; Setúbal, Sulamita S.; Tavares, Maria Naiara Macedo; Boeno, Charles Nunes; Ferreira, Alex Augusto Ferreira e; Paloschi, Mauro Valentino; Soares, Andreimar M.; Zamunér, Stella Regina; Zuliani, Juliana Pavan

doi:10.1016/j.cbi.2020.109347

Cited by 6 publications

(13 citation statements)

References 31 publications

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“…LED (Light Emitting Diode) Treatment. Cells were irradiated with a 945 nm infrared LED device (model Super Bright LEDs, Inc., St. Louis, MO, USA) immediately upon addition of the venom or toxin in the culture and LED was applied with direct contact to the bottom of the well plate in a continuous mode for 30 s [14][15][16]. The experiments were conducted in an environment with partial obscurity to not suffer interference from external light.…”

Section: Endotoxin Quantificationmentioning

confidence: 99%

“…The output power of the LED equipment was measured using the Laser Check power meter (MM Optics, São Carlos, Brazil). The LED parameters, low enough to avoid any thermal effect, were chosen based on previous studies [11,[14][15][16]. The experimental parameters for the LED are presented in Table 1.…”

Section: Endotoxin Quantificationmentioning

confidence: 99%

“…To verify the damage to the cell's plasma membrane, the LDH released into the cell culture supernatant was measured. In brief, TG-macrophages (2x10 BjV and both toxins, BthTX-I and BthTX-II, induce ROS production, and LED photobiomodulation reduces this effect [14,15]. According to the results obtained at the time interval studied, TG-macrophages stimulated with BjV or bothropstoxins did not alter the SOD activity.…”

Section: Cell Integrity Under Apocynin and Cccp3 Pharmacologicalmentioning

confidence: 99%

“…Liberation and LDH Release from TG-Macrophages Stimulated with BjV, BthTX-I, or BthTX-II. Pereira dos Reis et al [14] and Reis et al [15] showed that BjV as well as BthTX-I and BthTX-II induced ROS production and LED photobiomodulation was effective in decreasing this effect.…”

Section: Effect Of Led Photobiomodulation On Hydrogen Peroxidementioning

confidence: 99%

“…Pereira dos Reis et al [14], Reis et al [15], and Reis et al [16] showed that LED photobiomodulation also has beneficial properties in vitro. When the phototherapy was applied to the elicited macrophages under snake venom or toxins effects, they were protected from death.…”

Section: Introductionmentioning

confidence: 99%

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Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA2s

Reis

Setúbal

Ferreira

et al. 2022

BioMed Research International

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Photobiomodulation therapy associated with conventional antivenom treatment has been shown to be effective in reducing the local effects caused by bothropic venoms in preclinical studies. In this study, we analyzed the influence of photobiomodulation using light emitting diode (LED) on the oxidative stress produced by murine macrophages stimulated with Bothrops jararacussu venom and it isolated toxins BthTX-I and BthTX-II. Under LED treatment, we evaluated the activity of the antioxidant enzymes catalase, superoxide dismutase, and peroxidase as well as the release of hydrogen peroxide and the enzyme lactate dehydrogenase. To investigate whether NADPH oxidase complex activation and mitochondrial pathways could contribute to hydrogen peroxide production by macrophages, we tested the effect of two selective inhibitors, apocynin and CCCP3, respectively. Our results showed that LED therapy was able to decrease the production of hydrogen peroxide and the liberation of lactate dehydrogenase, indicating less cell damage. In addition, the antioxidant enzymes catalase, superoxide dismutase, and peroxidase increased in response to LED treatment. The effect of LED treatment on macrophages was inhibited by CCCP3, but not by apocynin. These findings show that LED photobiomodulation treatment protects macrophages, at least in part, by reducing oxidative stress caused B. jararacussu venom and toxins.

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Section: Endotoxin Quantificationmentioning

confidence: 99%

Section: Endotoxin Quantificationmentioning

confidence: 99%

Section: Cell Integrity Under Apocynin and Cccp3 Pharmacologicalmentioning

confidence: 99%

Section: Effect Of Led Photobiomodulation On Hydrogen Peroxidementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA2s

Reis

Setúbal

Ferreira

et al. 2022

BioMed Research International

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Photobiomodulation Therapy to Treat Snakebites Caused by Bothrops atrox

Carvalho,

Souza,

Melo

et al. 2024

JAMA Intern Med

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ImportanceBothrops venom acts almost immediately at the bite site and causes tissue damage.ObjectiveTo investigate the feasibility and explore the safety and efficacy of low-level laser therapy (LLLT) in reducing the local manifestations of B atrox envenomations.Design, Setting, and ParticipantsThis was a double-blind randomized clinical trial conducted at Fundação de Medicina Tropical Doutor Heitor Vieira Dourado, in Manaus, Brazil. A total of 60 adult participants were included from November 2020 to March 2022, with 30 in each group. Baseline characteristics on admission were similarly distributed between groups. Data analysis was performed from August to December 2022.InterventionThe intervention group received LLLT combined with regular antivenom treatment. The laser used was a gallium arsenide laser with 4 infrared laser emitters and 4 red laser emitters, 4 J/cm2 for 40 seconds at each application point.Main Outcomes and MeasuresFeasibility was assessed by eligibility, recruitment, and retention rates; protocol fidelity; and patients’ acceptability. The primary efficacy outcome of this study was myolysis estimated by the value of creatine kinase (U/L) on the third day of follow-up. Secondary efficacy outcomes were (1) pain intensity, (2) circumference measurement ratio, (3) extent of edema, (4) difference between the bite site temperature and that of the contralateral limb, (5) need for the use of analgesics, (6) frequency of secondary infections, and (7) necrosis. These outcomes were measured 48 hours after admission. Disability assessment was carried out from 4 to 6 months after patients’ discharge. P values for outcomes were adjusted with Bonferroni correction.ResultsA total of 60 patients (mean [SD] age, 43.2 [15.3] years; 8 female individuals [13%] and 52 male individuals [87%]) were included. The study was feasible, and patient retention and acceptability were high. Creatine kinase was significantly lower in the LLLT group (mean [SD], 163.7 [160.0] U/L) 48 hours after admission in relation to the comparator (412.4 [441.3] U/L) (P = .03). Mean (SD) pain intensity (2.9 [2.7] vs 5.0 [2.4]; P = .004), circumference measurement ratio (6.6% [6.6%] vs 17.1% [11.6%]; P &lt; .001), and edema extent (25.8 [15.0] vs 40.1 [22.7] cm; P = .002) were significantly lower in the LLLT group in relation to the comparator. No difference was observed between the groups regarding the mean difference between the bite site temperature and the contralateral limb. Secondary infections, necrosis, disability outcomes, and the frequency of need for analgesics were similar in both groups. No adverse event was observed.Conclusions and RelevanceThe data from this randomized clinical trial suggest that the use of LLLT was feasible and safe in a hospital setting and effective in reducing muscle damage and the local inflammatory process caused by B atrox envenomations.Trial RegistrationBrazilian Registry of Clinical Trials Identifier: RBR-4qw4vf

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Bothrops atrox mice experimental envenoming treatment using light-emitting diode (led) as an adjunct therapy to conventional serum therapy

et al. 2023

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Effect of light emitting diode photobiomodulation on murine macrophage function after Bothrops envenomation

Cited by 6 publications

References 31 publications

Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA2s

Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA2s

Photobiomodulation Therapy to Treat Snakebites Caused by Bothrops atrox

Bothrops atrox mice experimental envenoming treatment using light-emitting diode (led) as an adjunct therapy to conventional serum therapy

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