Effect of light-emitting diode (ʎ 627 nm and 945 nm ʎ) treatment on first intention healing: Immunohistochemical analysis

Abstract

Collagen I is not only responsible for maintaining the integrity of most tissues due to its mechanical properties, but also for its active participation in the functionality of tissues because of its interaction with cells present in the extracellular matrix. The synthesis of collagen begins with tissue injury and remains until the end of the healing process. The use of non-coherent light for healing processes is still understudied. This procedure stands out as a biostimulation method for tissue repair, which increases local circulation, cell proliferation, and collagen synthesis. This study sought to quantify collagen I in the healing process after the treatment of wounds with the light-emitting diode (LED) treatment. The histologic analysis with tissue samples stained with picrosirius red showed a statistical difference between the positive controls, LED 627 and LED 945 nm groups; the group treated with LED 627 nm showed a predominance of mature collagen. The immunohistochemical analysis showed a statistically significant high concentration of collagen I in the LED 945 nm group. The irradiation of wounds with the higher wavelength (945 nm) used in the study produced the best activity of collagen I formation in experimental model.

The photobiomodulation (658, 830 and 904nm) on wound healing in histomorphometric analysis

“…Chiarotto et al [20] found similar results using energy density of 4.48 J/cm2 in animals submitted to second degree burn, with a greater effectiveness of the laser 670 nm compared to 830 nm. Kerppers et al [25] reported that small doses of laser fractions at different times are more effective than higher doses in a single application because it sustains mitochondrial activity.…”

“…For the application of the PBM we used the HTM Compact ® Laser (Amparo, SP, Brazil, License: 80212480005) with a frequency of 60 Hz, power supply, and a maximum current of 54 mA. Table 1 presents the PBM parameters used with the same energy density standardized for the three groups (4 J/cm²) [24][25][26][27]. The application was given point by point [28], with the probe in perpendicular contact to the edge of the wound (0.5 cm), totaling 12 points ( Figure 1).…”

O uso terapêutico do LASER de Baixa Intensidade (LBI) em algumas patologias e sua relação com a atuação na Fonoaudiologia

“…Muitos pacientes com disfagia apresentam odinofagia, problemas gástricos, doenças orais que dificultam o processo terapêutico (candidíase, herpes, gengivites, entre outros), edema, alterações de tônus e mobilidade dos músculos responsáveis pelo preparo e impulsionamento do bolo alimentar 23,24 . O LBI poderia auxiliar o fonoaudiólogo nesses casos com o uso do LASER vermelho para analgesia, ação anti-inflamatória e antimicrobiana 4,10,17 . O LASER vermelho no modo ILIB, aplicado na artéria radial tem efeito sistêmico e pode ser aplicado sem riscos ao paciente que muitas vezes está debilitado.…”

Light-emitting diodes in dermatology: stimulation of wound healing

“…Promieniowanie emitowane przez diody LED, działając m.in. poprzez stymulację proliferacji i dojrzewania fibroblastów, wpływa na zwiększenie syntezy kolagenu, elastyny, glikoprotein i glikozoamin oraz regulację aktywności metaloproteinaz w tkankach [13][14][15]. Pod wpływem promieniowania czerwonego i podczerwonego dochodzi też do degranulacji mastocytów i uwalniania m.in.…”

“…Terapia światłem małych mocy wpływa na wszystkie trzy fazy gojenia ran: fazę zapalną, w której komórki zapalne migrują do rany, fazę proliferacyjną z pobudzeniem fibroblastów i makrofagów oraz fazę dojrzewania, w której nastę-puje proces przebudowy zagojonej już rany, aby stała się bardziej wytrzymała. Pod wpływem właściwie dobranego promieniowania świetlnego następuje zwiększenie produkcji oraz aktywacji fibroblastów i makrofagów, pobudzenie leukocytów i przyspieszenie tworzenia kolagenu oraz neowaskularyzacji [13,19]. Rany, w których przy stosowaniu klasycznych metod leczniczych proces gojenia nie przebiegał właściwie, ulegały zabliźnieniu po zastosowaniu naświetlania diodami LED emitującymi promieniowanie z zakresu widzialnego i bliskiej podczerwieni.…”