Effect of light Intensity and Temperature on in vitro Somatic Embryos Germination Stage in Date Palm

Abstract

The aim of this study is to detect the extent of physical factors (light intensity and temperature) effect on proliferation and germination of date palm somatic embryos in vitro. The treatments of this study were carried out by using two degrees of temperature (18 ± 2 ºC & 28 ± 2 ºC) with four levels of light intensity (0, 20, 40 and 60 μmol m-2 s-1). The obtained results revealed the following: The results showed that there are significant differences between the eight treatments of physical factors under study as the results monitored that the highest average for No. of somatic embryos germination was for the explants cultured at light intensity of 40 μmol m-2 s-1 and under low temperature of 18±2ºC, whereas the highest average for germination lengths was for the explants cultured at light intensity of 40 μmol m-2 s-1 and high temperature of 28±2ºC all over the study. While, the lowest values were for the explants cultured at total darkness.

Effect of dark incubation in germination of indirect date palm somatic embryos and conversion into plantlets

“…The incubation under light gave the highest germination of date palm somatic embryos. While embryos cultured at total darkness gave the lowest values for germination and number of somatic embryos (Shehata et al 2015). The central focus of research in many palm types is somatic embryo multiplication protocols, but germination and conversion of embryos remain badly studied (Ree and Guerra 2015).…”