Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants

Hernández, Karel; Moreno‐Romero, Jordi; Torre, Martha Hernández de la; Pérez, Claudia A.; Leal, Darcy Ríos; Martínez‐Garcia, Jaime F.

doi:10.1016/j.phytochem.2021.113027

Cited by 20 publications

(8 citation statements)

References 55 publications

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“…Studies have shown that high light intensity increases the levels of stevioside and rebaudioside A and, thus, alters the production of SvGls. High levels of SvGls were also confirmed by analyzing the gene expression of SrKA13H, SrUGT74G1, and SrUGT76G1, which increased at higher light intensity [25]. The stevia plant is a short-day plant with a critical daylight requirement of 11-12 h to induce flowering.…”

Section: Alteration In Light and Photoperiodmentioning

confidence: 79%

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Rai

Han

2022

Applied Sciences

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Steviol glycosides (SvGls) are plant secondary metabolites belonging to the class diterpenes. SvGls naturally derived from non-calorie sugar have therapeutic properties for diabetics, carcinogens, and anti-microbials. Over the past few years, SvGls have been extensively researched because of their extraordinary sweetness without side effects. SvGls are classified into several types based on the number of sugar bases attached to the steviol. Due to the difference in glycosylation, different SvGls have different sensory properties. One of the main obstacles is the mass production of SvGls. Novel physical and chemical treatments and advanced biotechnological approaches are introduced to increase the production of SvGls. Extraction of high-purity SvGls from plants is difficult, so researchers have manipulated the metabolic pathways of engineered microorganisms for bulk production of SvGls. There are many aspects related to the biochemistry and metabolism of SvGls, but their functional relationship with the S. rebaudiana is not well understood. Thus there is a need for in-depth research in this area. This review provides the readers with an overview of the research gaps and possible promising methodologies that can be utilized in the future. To trigger more research, this review encompasses the recent developments in SvGls production and marketing.

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Section: Alteration In Light and Photoperiodmentioning

confidence: 79%

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Rai

Han

2022

Applied Sciences

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“…In this case, a more southerly slope direction, higher TK, and lower Na content in the soil correspond to a more abundant flora of bioactive compounds. Among them, south-facing means more sunshine, which may be associated with the facilitation that many plants improve SMs outcome under the enhancement of light exposure or UV stress ( Amarasinghe et al., 2015 ; Tohge et al., 2016 ; Hernández et al., 2022 ). The effects of different contents of soil total K on the regulation of SMs in plants have also been reported ( Liu et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Geographical equations of Swertia mussotii bioactivities: evidence from the western Sichuan region of China

et al. 2023

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Swertia mussotii is the most authentic raw material used in Tibetan medicine in China for its various bioactivities. This natural medicine resource is at risk of being exhausted due to the double interference of climate change and anthropogenic over-collection. Little is known about habitat characteristics and the crucial environmental factors that influence the levels of active ingredients. The goal of this study is to understand the variability in the bioactive compound content of a wide range of wild S. mussotii as it adapts to changing environmental conditions. The target compound content of the whole plant material was analyzed with the environmental explanatory variables of the field sample sites using a constrained ordination method for their correlation analysis. The results show that 16.3 percent of the sampled wild S. mussotii populations with the highest bioactive content can be grouped into the elite type. The most prominent environmental variables affecting the content of major bioactive products include altitude, aspect, soil TK content, Fe content, and C/N and N/P ratios. Altitude and aspect put indirect effects that are mediated by plant height and density, N/P ratio puts a direct effect, while soil TK content, Fe content and C/N ratio have both direct and indirect effects on the bioactivity of S. mussotii. In addition to the total negative effects of altitude and C/N ratio, the remaining factors play a driving role. These findings demonstrate variation by geographical conditions across S. mussotii accessions for physiologic responses and secondary compounds in wild populations. The knowledge gained from this study can be used for environmental and plant physiology research, efficient collection of naturally active compounds, and conservation strategies for rare natural plant resources.

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“…Furthermore, the light regime is considered able to influence steviol glycosides production. However, the optimal light regime to improve their content is currently under debate [ 30 ]. As evidence for this aspect, in our work, from a qualitative point of view, the growth conditions in the underwater biospheres did not alter the chemical profile in terms of non-volatile secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

Resilience of Stevia rebaudiana (Bertoni) Bertoni in the Underwater Biospheres of Nemo’s Garden®: Adaptation to New Cultivation Systems

et al. 2022

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The Nemo’s Garden® project is an alternative production system for areas with scarce cultivable land but significant presence of water; thus, it is an interesting intervention to address the climate crisis. This work aimed to evaluate the micromorphological, biochemical, and phytochemical characteristics of Stevia rebaudiana (Bertoni) Bertoni grown underwater compared to the terrestrial specimens. The micromorphological analyses, performed on the leaves using light microscopy, fluorescence microscopy, and scanning electron microscopy, evidenced a general uniformity of the trichome morphotype and distribution pattern. The histochemical investigation indicated the simultaneous presence of terpenes and polyphenols in the trichome secreted material from the underwater samples and a prevailing polyphenolic content in the terrestrial specimens; this was also confirmed by biochemical analyses (26.6 mg GAE/g DW). The characterization of non-volatile components, performed using HPLC–MS, showed similar chemical profiles in all the samples, which were characterized by phenolic compounds and steviol glycosides. The volatile compounds, evaluated using HS-SPME coupled with GC–MS, showed sesquiterpene hydrocarbons as the main class in all the analyzed samples (80.1–93.9%). However, the control plants were characterized by a higher content of monoterpene hydrocarbons (12.1%). The underwater biosphere environment did not alter S. rebaudiana micro-morphological characters, although slight qualitative changes were evidenced for the compounds produced as a response to the growth conditions.

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Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants

Cited by 20 publications

References 55 publications

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Critical Review on Key Approaches to Enhance Synthesis and Production of Steviol Glycosides: A Blueprint for Zero-Calorie Sweetener

Geographical equations of Swertia mussotii bioactivities: evidence from the western Sichuan region of China

Resilience of Stevia rebaudiana (Bertoni) Bertoni in the Underwater Biospheres of Nemo’s Garden®: Adaptation to New Cultivation Systems

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