Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

Souza, Denys Matheus Santana Costa; Fernandes, Sérgio Bruno; Silva, Eduardo Oliveira; Duarte, Vinícius Politi; Gonçalves, Douglas Santos; Carvalho, Dulcinéia de; Teixeira, Gustavo Leal; Brondani, Gilvano Ebling

doi:10.1007/s11627-021-10237-6

Cited by 9 publications

(1 citation statement)

References 62 publications

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“…Reports are available regarding the early phases of in vitro introduction (Avelar et al, 2020;Molinari et al, 2021), bud multiplication (Máximo et al, 2018;Carvalho et al, 2019;Souza et al, 2020a), shoot elongation (Souza et al, 2020b;Zorz et al, 2020), rooting and acclimatization of microplants (Souza et al, 2019;Miranda et al, 2020). Tissue rejuvenation/reinvigoration can be optimized through asexual propagation, which provides higher levels of clonal productivity and adventitious rooting (Wendling et al, 2014;Brondani et al, 2018;Abiri et al, 2020;Souza et al, 2022).…”

Section: Clonal Propagation By Micropropagation Technique Of Eucalypt...mentioning

confidence: 99%

In Vitro Multiplication of Eucalyptus pilularis and Eucalyptus grandis x E. urophylla (Urograndis Eucalypt): Effect of Light Quality in Temporary Immersion Bioreactor

Souza,

Martins,

Fernandes

et al. 2022

MJST

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Light quality is an important factor for in vitro development of explants in a bioreactor system. Based on the need to optimize, this study aimed to evaluate the in vitro multiplication of Eucalyptus pilularis and urograndis eucalypt using different light quality in a temporary immersion bioreactor (TIB) system. Different spectral qualities on in vitro multiplication were evaluated using three light sources, namely fluorescent lamp (white light), red and blue. Shoot length, number of buds, fresh weight per explant, vigor and hyperhydricity were evaluated according to an established scoring scale at 30 days. The results showed that fluorescent white light was the most appropriate for use in the in vitro multiplication of E. pilularis, and blue light for the urograndis eucalypt clone resulting in a greater shoot length (1.46 cm; 2.41 cm), number of buds (2.25; 10.20), vigor (1.8; 1.3) and fresh weight per explant (86.9 mg; 449 mg). The results can be applied to optimize clonal microplant production on a commercial scale.

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