Effect of Lignin-Containing Media on Growth of Medicinal Mushroom Lentinula Edodes

Matjuškova, Nataļja; Okmane, Laura; Zaļā, Dzintra; Rozenfelde, Linda; Puķe, Māris; Krūma, Irēna; Vederņikovs, Nikolajs; Rapoport, Alexander

doi:10.1515/prolas-2017-0007

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…Dyes decolorization in agar medium Decolorization of RBBR and AB129 dyes in twolayered agar media containing lignin in bottom layer and dyes in top layer were preliminary evaluated to screen the dye degradation activity of the fungal strains. Addition of lignin in cultivation media can increase mycelium biomass and laccase activity in Basidiomycota (Matjuškova et al, 2017). Fungal growth and dye decolorization were assessed during the screening.…”

Section: Resultsmentioning

confidence: 99%

Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi

et al. 2021

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Water pollution by dyes represents from dyestuff industry becomes an environmental concern. Finding new isolates capable of decolorizing these dyes is important. The study aimed to assess the new isolates of white-rot fungi (WRF) as decolorizing agent of anthraquinone and azo dyes. Decolorization assay were conducted in Agar plates and liquid medium. During the decolorization, laccase activities produced by the fungal strains were analyzed. Identification of the fungal strains were investigated using molecular DNA analysis. The results showed that isolates M3, H18, and GP1 were able to decolorize anthraquinone and azo dyes in Agar and liquid medium. Based on DNA analysis, isolates M3, H18, and GP1 have the similarity to Trametes sanguinea, Trametes polyzona, and Neofomitella guangxiensis, respectively. Among the fungi, T. polyzona H18 exhibited high decolorization ability (70â€“90%) to the dyes (100 mg/L) after 96-hours incubation. Laccase activity was fluctuated during the reactions with tendency to increase at the beginning until its peak, then decreased at the end of incubation. This study demonstrated the potential of the new isolates from Indonesia to decolorize anthraquinone and azo dyes. The results of the study can provide an alteranative for bioremediation agents of contaminated water by synthetic dyes.

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Section: Resultsmentioning

confidence: 99%

Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi

et al. 2021

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“…It was shown that the addition of lignocelluloses residues to culture media could influence the increase in laccase activity of white-rot fungi by several-fold [10,30,46,47]. Additionally, our experiments showed that laccase production increased under submerged fermentation with L. edodes 3565 after the addition of pretreated wheat straw [5]. At the same time, we must note that in previous experiments, where all of the results were obtained with the addition of lignocellulosic residues, we used lignin waste obtained after furfural and bioethanol production from colza straw.…”

Section: Native Pagementioning

confidence: 57%

“…This process generates the most recalcitrant lignin residues that must undergo further biodegradation to make the production of furfural and bioethanol or lipids as a waste-less process. The lignin waste may be used to improve the growth of medicinal mushrooms and to produce laccase-containing enzymes complexes [5,6].…”

Section: Introductionmentioning

confidence: 99%

Effect of Pretreated Colza Straw on the Growth and Extracellular Ligninolytic Enzymes Production by Lentinula edodes and Ganoderma lucidum

et al. 2021

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Lentinula edodes 3565 and Ganoderma lucidum 9621 were compared for their ability to produce lignocellulolytic enzymes in submerged (SM) and surface liquid (SL) fermentation of hydrolysed colza straw lignin waste that remained after the production of furfural and bioethanol (CS lignin). Application of cultivated mushrooms to dispose of pretreated colza straw agricultural waste is an approach to decrease the quantity of residual lignin while simultaneously obtaining active substances, e.g., the ligninolytic enzyme complex from mycelium. The effect of adding CS lignin to culture media on the yield of L. edodes and G. lucidum mycelium and extracellular laccase activity was studied. It was revealed that the mycelial growth of G. lucidum on solid media was significantly improved by adding CS lignin. Laccase activity during SL cultivation of L. edodes on medium with CS lignin gradually increased over the experiment starting on day 21 and peaked at 520 U/mL on day 28. G. lucidum expressed the maximum laccase activity, 540 U/mL, during the first 14 days of mycelium SM cultivation. Extracellular laccase activity was enhanced about 35- to 40-fold at cultivation of L. edodes and about 10- to 15-fold in the case of G. lucidum by supplementing liquid culture media with CS lignin.

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“…In this screening process, the lignin-containing in the medium will trigger the fungal isolate extracellularly produce the ligninolytic enzyme and oxidize the lignin. The previous study reported that lignin addition in media increased the fungal cell mass and ligninolytic enzyme activity of whiterot fungi (Thorn et al 1996;Ferrara et al 2002;Anita et al 2011;Matjuškova et al 2017). The ligninolytic enzyme extracellularly released by fungi in the medium then decolorized the RBBR dye via an oxidizing process (Moreira-Neto et al 2013;Falah et al 2018).…”

Section: Isolation and Screening Of Fungi Ligninolytic Enzymeproducermentioning

confidence: 96%

Bioprospecting three newly isolated white-rot fungi from Berbak-Sembilang National Park, Indonesia for biodecolorization of anthraquinone and azo dyes

et al. 2022

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Abstract. Nurhayat OD, Ardiati FC, Ramadhan KP, Anita SH, Okano H, Watanabe T, Yanto DHY. 2021. Bioprospecting three newly isolated white-rot fungi from Berbak-Sembilang National Park, Indonesia for biodecolorization of anthraquinone and azo dyes. Biodiversitas 22: 613-623. Extensive use of textile dyes without proper wastewater treatment may jeopardize the water environment. In this study, bioprospecting newly isolated white-rot fungi from Berbak-Sembilang National Park for decolorizing four synthetic dyes was investigated. A total of 108 wood-decaying fungi were screened by using selective media, resulting in three isolates as the most promising fungal strains (BRB 11, BRB 73, BRB 81). BRB 81 had the best ability to decolorize 91.4% of AB129 and 77.8% of RB5 within 96 hours while the highest removal of RBBR and AO7 was performed by BRB11 around 60% and 37.6%, respectively. The enzymatic degradation was assumed to involve the decolorization process as laccase activities were observed with the highest around 116 UL-1. Based on molecular identification, these three fungal isolates were identified as Phellinus noxius BRB 11, Ceriporia lacerata BRB 81, and Leiotrametes menziesii BRB 73, respectively. In conclusion, P. noxius BRB 11, L. menziesii BRB 73, and C. lacerata BRB 81 could be used as biological agents in textile wastewater treatment and thus, it is important to conserve them as a part of the biodiversity within the local biosphere reserve.

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Effect of Lignin-Containing Media on Growth of Medicinal Mushroom Lentinula Edodes

Abstract: Contributed by Alexander Rapoport

Cited by 5 publications

References 17 publications

Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi

Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi

Effect of Pretreated Colza Straw on the Growth and Extracellular Ligninolytic Enzymes Production by Lentinula edodes and Ganoderma lucidum

Bioprospecting three newly isolated white-rot fungi from Berbak-Sembilang National Park, Indonesia for biodecolorization of anthraquinone and azo dyes

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