Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested
            <i>Corynebacterium glutamicum</i>
            R

Sakai, Shinsuke; Tsuchida, Yoshiki; Nakamoto, Hiroka; Okino, Shohei; Ichihashi, Osamu; Kawaguchi, Hideo; Watanabe, Takashi; Inui, Masayuki; Yukawa, Hideaki

doi:10.1128/aem.01858-07

Cited by 19 publications

(20 citation statements)

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“…These growth-arrested but metabolically active cells permit high product yields while suppressing byproduct formation and can be used at higher cell densities than is possible under aerobic conditions, leading to high volumetric productivities (17,18,20,32). In addition to high resistance to lignocellulose hydrolysate-derived fermentation inhibitors during oxygen deprivation (37), C. glutamicum strains with the added capability of utilizing pentose and hexose sugars simultaneously have been constructed (39,40). Based on these observations, an innovative and versatile bioprocess that is simple, economical, and environment friendly can be developed to produce commodity chemicals.…”

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confidence: 99%

Improvement of the Redox Balance Increasesl-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions

Hasegawa

Uematsu

Natsuma

et al. 2012

Appl Environ Microbiol

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119

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Production of L-valine under oxygen deprivation conditions by Corynebacterium glutamicum lacking the lactate dehydrogenase gene ldhA and overexpressing the L-valine biosynthesis genes ilvBNCDE was repressed. This was attributed to imbalanced cofactor production and consumption in the overall L-valine synthesis pathway: two moles of NADH was generated and two moles of NADPH was consumed per mole of L-valine produced from one mole of glucose. In order to solve this cofactor imbalance, the coenzyme requirement for L-valine synthesis was converted from NADPH to NADH via modification of acetohydroxy acid isomeroreductase encoded by ilvC and introduction of Lysinibacillus sphaericus leucine dehydrogenase in place of endogenous transaminase B, encoded by ilvE. The intracellular NADH/NAD ؉ ratio significantly decreased, and glucose consumption and L-valine production drastically improved. Moreover, L-valine yield increased and succinate formation decreased concomitantly with the decreased intracellular redox state. These observations suggest that the intracellular NADH/NAD ؉ ratio, i.e., reoxidation of NADH, is the primary rate-limiting factor for L-valine production under oxygen deprivation conditions. The L-valine productivity and yield were even better and by-products derived from pyruvate further decreased as a result of a feedback resistance-inducing mutation in the acetohydroxy acid synthase encoded by ilvBN. The resultant strain produced 1,470 mM L-valine after 24 h with a yield of 0.63 mol mol of glucose ؊1 , and the L-valine productivity reached 1,940 mM after 48 h.

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confidence: 99%

Improvement of the Redox Balance Increasesl-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions

Hasegawa

Uematsu

Natsuma

et al. 2012

Appl Environ Microbiol

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119

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“…RSY Production (%) = 100 (RSY /Total theoretical RSY) (1) Total theoretical RSY = (Biomass concentration) (dry biomass) (hemicellulose) (2) For enzyme hydrolysis filter paper procedures were used to determine the activities of the cellulase and cellobiase. Comercial cellulose 1500 and multifect CXB enzymes were used for the enzymatic hydrolysis step.…”

Section: Methodsmentioning

confidence: 99%

“…The chance to develop a sustainable economic bioethanol project implies fast growing crop adapted to the climatic and soils conditions of a specific area and a lignocellulosic biomass efficient technology conversion to fermentable sugars at lower costs [1,2]. It means to compete with gasoline; the cost production of ethanol from biomass should be competitive [3].…”

Section: Introductionmentioning

confidence: 99%

Efecto del tratamiento previo de ácido diluido - peróxido en la hierba napier (Pennisetum purpureum schumach) para mejorar la reducción Producción de azúcar por hidrólisis enzimática

Bohórquez

González

Reina

2014

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The napier grass (Pennisetum purpureum Schumach.) is a potential feedstock biomass for conversion to ethanol, bio-energy or high added value products. Variation in prehydrolysis parameters (time, acid strength, biomass %, particle size) and enzymatic saccharification conditions were examined for conversion of napier grass into fermentable sugars. A pretreatment of the water insoluble substrate (WIS H 2 SO 4 ) at 15.1 % of reducing sugar yield (RSY) was done with peroxide (H 2 0 2 ) at 122 °C. The relationship between RSY and the acid process parameters are described by a mathematical model derived from the experimental data.The peroxide pretreatment at 0.7 % (w/w), 4.75 mm particle size, 8% (w/v) biomass concentration, improved the RSY production from enzymatic hydrolysis in 97.6 % after 75 min. The enzymatic hydrolysis produced 287.81 mg/(g initial dry sample) of glucose and 245.81 mg/(g initial dry sample) of Xylose. Therefore, it was concluded that combination peroxide -acid pretreatment is an effective and environmentally friendly method for the enzyme hydrolysis of napier grass.

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“…Available from and , Cited 18 March 2014). Besides amino acids, the diamines cadaverine and putrescine (Mimitsuka et al, 2007; Schneider and Wendisch, 2010) and the alcohols ethanol and isobutanol (Sakai et al, 2007; Blombach and Eikmanns, 2011), among others, can be produced from sugars by recombinant C. glutamicum strains. Furthermore, access of C. glutamicum to alternative feed stocks like glycerol from the biodiesel process (Meiswinkel et al, 2013), pentoses from lignocellulosics (Gopinath et al, 2011), amino sugars (Uhde et al, 2013; Matano et al, 2014), starch (Seibold et al, 2006), and β-glucans (Tsuchidate et al, 2011) has been engineered.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the IPP Precursor Supply for the Production of Lycopene, Decaprenoxanthin and Astaxanthin by Corynebacterium glutamicum

Heider

Wolf

Hofemeier

et al. 2014

Front. Bioeng. Biotechnol.

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The biotechnologically relevant bacterium Corynebacterium glutamicum, currently used for the million ton-scale production of amino acids for the food and feed industries, is pigmented due to synthesis of the rare cyclic C50 carotenoid decaprenoxanthin and its glucosides. The precursors of carotenoid biosynthesis, isopenthenyl pyrophosphate (IPP) and its isomer dimethylallyl pyrophosphate, are synthesized in this organism via the methylerythritol phosphate (MEP) or non-mevalonate pathway. Terminal pathway engineering in recombinant C. glutamicum permitted the production of various non-native C50 and C40 carotenoids. Here, the role of engineering isoprenoid precursor supply for lycopene production by C. glutamicum was characterized. Overexpression of dxs encoding the enzyme that catalyzes the first committed step of the MEP-pathway by chromosomal promoter exchange in a prophage-cured, genome-reduced C. glutamicum strain improved lycopene formation. Similarly, an increased IPP supply was achieved by chromosomal integration of two artificial operons comprising MEP pathway genes under the control of a constitutive promoter. Combined overexpression of dxs and the other six MEP pathways genes in C. glutamicum strain LYC3-MEP was not synergistic with respect to improving lycopene accumulation. Based on C. glutamicum strain LYC3-MEP, astaxanthin could be produced in the milligrams per gram cell dry weight range when the endogenous genes crtE, crtB, and crtI for conversion of geranylgeranyl pyrophosphate to lycopene were coexpressed with the genes for lycopene cyclase and β-carotene hydroxylase from Pantoea ananatis and carotene C(4) oxygenase from Brevundimonas aurantiaca.

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Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

Cited by 19 publications

References 0 publications

Improvement of the Redox Balance Increasesl-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions

Improvement of the Redox Balance Increasesl-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions

Efecto del tratamiento previo de ácido diluido - peróxido en la hierba napier (Pennisetum purpureum schumach) para mejorar la reducción Producción de azúcar por hidrólisis enzimática

Optimization of the IPP Precursor Supply for the Production of Lycopene, Decaprenoxanthin and Astaxanthin by Corynebacterium glutamicum

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