Effect of linolenyl alcohol on the in-vitro growth of the oral bacterium Streptococcus mutans

Crout, Richard J.; Gilbertson, John R.; Gilbertson, John D.; Platt, D; Langkamp, H.H.; Connamacher, Robert H.

doi:10.1016/0003-9969(82)90008-5

Cited by 9 publications

(10 citation statements)

References 22 publications

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“…In our laboratory, S. mutans BHT has been studied in detail as an example of the viridans group streptococci. The procedures used to culture this bacterium and to estimate growth from turbidity measurement have been published (4). The effect of the long-chain alcohol, an aminoglycoside, or a combination of these two substances on the growth of S. mutans BHT and other species of streptococci was determined by the following method.…”

Section: Methodsmentioning

confidence: 99%

“…The cultures were incubated at 37°C under aerobic conditions, and growth was assessed by turbidity measurements and confirmed by determination of the number of CFU. Colony counts were assessed before and at 4,8,24, and 48 h after the addition of the long-chain alcohol or the aminoglycoside or both. A 0.…”

Section: Methodsmentioning

confidence: 99%

“…Prep, Inc., Elysian, Minn. Purity was assessed by thin-layer chromatography and gas-liquid chromatography (4). In each instance the alcohols were at least 99% pure.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, low concentrations of linolenyl alcohol have been shown to inhibit growth of Streptococcus mutans and other gram-positive bacteria in cultures (4). In this instance, the alcohol was proposed to act as a molecular spacer between the hydrocarbon chains of the acyl-phosphatides, changing the structure of the plasma membrane and increasing its permeability (4).…”

mentioning

confidence: 99%

“…In this instance, the alcohol was proposed to act as a molecular spacer between the hydrocarbon chains of the acyl-phosphatides, changing the structure of the plasma membrane and increasing its permeability (4).…”

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Use of lipids to potentiate the antibacterial activity of aminoglycosides

Gilbertson¹,

Langkamp²,

Connamacher³

et al. 1984

Antimicrob Agents Chemother

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Linolenyl alcohol has been shown to inhibit the in vitro growth of several species of gram-positive bacteria. Since the double bonds in linolenyl alcohol could undergo autooxidation, the antimicrobial activities of saturated primary alcohols of similar molecular sizes against Streptococcus mutans BHT were evaluated. Tridecan-l-ol was identified as the most active compound, eliciting a bacteriostatic effect at concentrations at which growth occurred in the presence of other saturated alcohols or linolenyl alcohol. Evidence is also presented that the combined use of tridecan-l-ol and gentamicin sulfate produces a synergistic effect that is bactericidal to S. mutans BHT. A similar bactericidal response was observed when tridecan-l-ol was combined with other aminoglycosides or tested alone against other strains of S. mutans.When used alone, the aminoglycosides are not active in vitro against enterococci (2, 3). However, when an aminoglycoside is combined with penicillin or other cell-wallactive agents, a bactericidal response is achieved (3, 7). The enhancement by penicillin of the effectiveness of aminoglycosides is due to increased intracellular penetration by the aminoglycoside (7). This permeability change could result from the binding of the ,-lactam antibiotic to proteins associated with cell wall synthesis (7, 10) or from a change in the cell wall structure (8).Recently, low concentrations of linolenyl alcohol have been shown to inhibit growth of Streptococcus mutans and other gram-positive bacteria in cultures (4). In this instance, the alcohol was proposed to act as a molecular spacer between the hydrocarbon chains of the acyl-phosphatides, changing the structure of the plasma membrane and increasing its permeability (4).Since the combination of penicillin with an aminoglycoside elicits an enhanced antibacterial response, perhaps a similar potentiation could be obtained upon combination of an aminoglycoside and a long-chain primary alcohol. S. mutans BHT or other strains were used as examples of the streptococcal species. Several primary aliphatic alcohols were compared with linolenyl alcohol to identify the compound with the greatest antibacterial activity. This compound, tridecan-1-ol, was combined with gentamicin and several other aminoglycosides to ascertain whether antibacterial activity could be potentiated.MATERIALS AND METHODS Growth assay. In our laboratory, S. mutans BHT has been studied in detail as an example of the viridans group streptococci. The procedures used to culture this bacterium and to estimate growth from turbidity measurement have been published (4). The effect of the long-chain alcohol, an aminoglycoside, or a combination of these two substances on the growth of S. mutans BHT and other species of streptococci was determined by the following method.Stock solutions of the aminoglycosides were prepared in deionized water and sterilized by filtration through a membrane filter (pore size, 0.22 ,um; Millipore Corp., Bedford, * Corresponding author. t Deceased.Mass.). A sample of the stoc...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Prep, Inc., Elysian, Minn. Purity was assessed by thin-layer chromatography and gas-liquid chromatography (4). In each instance the alcohols were at least 99% pure.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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