Effect of Lipid a and Fatty Acids on Transport Activities of Membrane Vesicles From Escherichia Coli

Lai, Jiunu-Shyong; Okuda, Shinichi; Abe, Hiroshi; Takahashi, Hiroki

doi:10.2323/jgam.22.85

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…The lack of inhibition in these experiments means that the phosphotransferase system is not directly affected by parabens, and that membrane permeability-at least for glucose-is not reduced. No explanation is offered for the stimulatory effect of propyl and butyl paraben on glucose uptake, but a similar observation has been made by Lai et al (1976), who noted a slight stimulation of glucose uptake in Esch. coli vesicles subjected to the action of different fatty acids.…”

Section: Discussionmentioning

confidence: 67%

Inhibition of Growth and Uptake Processes in Bacteria by Some Chemical Food Preservatives

Eklund¹

1980

Journal of Applied Bacteriology

106

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The effect on growth and uptake processes of some common chemical food preservatives [benzoate, sorbate, propionate and alkyl esters of p‐hydroxybenzoic acid (parabens)] has been studied in strains of Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. For parabens. the inhibitory action on growth and amino acid uptake in whole cells and bacterial membrane vesicles followed similar dose‐response curves. Growth inhibition caused by parabens appears to be a consequence of transport inhibition. For benzoate, sorbate and propionate, uptake inhibition seems inadequate to explain growth inhibition.

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Section: Discussionmentioning

confidence: 67%

Inhibition of Growth and Uptake Processes in Bacteria by Some Chemical Food Preservatives

Eklund¹

1980

Journal of Applied Bacteriology

106

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“…Fatty acids not only inhibit growth (10,20,21,27), but they also kill (11,21,22) and lyse (21) gram-positive and, to a lesser extent, gram-negative bacteria. The bacteriostatic activity of fatty acids has been ascribed to destruction of the proton motive force across the cell membrane (24,25), leading to the loss of respiratory activity (13,34,36), the inhibition of substrate uptake (13,23,35,36), or the decrease in intracellular content of ATP (34,36).…”

mentioning

confidence: 99%

Involvement of autolysin in cellular lysis of Bacillus subtilis induced by short- and medium-chain fatty acids

Tsuchido¹,

Hiraoka²,

Takano³

et al. 1985

J Bacteriol

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The addition of saturated C6, C8, C1o, and C12 fatty acids appeared to lyse actively growing cells of Bacillus subtilis 168, as judged by a decrease in the optical density of the culture. Of these fatty acids, dodecanoic acid was the most effective, with 50% lysis occurring in about 30 min at a concentration of 0.5 mM. These conditions also decreased the amount of peptidoglycan estimated by the incorporated radioactivity of N-acetyl-D-[1-'4C]glucosamine. At concentrations above 1 mM, however, bacterial lysis was not extensive. Dodecanoic acid did not affect autolysis of the cell wall. The Iytic action of dodecanoic acid was greatly diminished in cells in which protein synthesis was inhibited and in an autolytic enzyme-deficient mutant. The results suggest that fatty acid-induced lysis of B. subtilis 168 is due to the induction of autolysis by an autolytic enzyme rather than massive solubilization of the cell membrane by the detergent-like action of the fatty acids.

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“…The ability of purified M. pneumoniae membranes to induce cell damage has been evaluated with several different assay systems and membrane preparation methods (10,12,14; M. G. Gabridge and R. B. Polisky, submitted for publication). In view of the wide variety of enzymatic activities associated with mycoplasma membrane proteins (28,30), as well as the cytotoxic activity of lipids (23,25), some form of membrane-associated cytopathic activity was not surprising. Still to be resolved, however, was the manner in which mycoplasma membranes physically interacted with epithelial cells.…”

mentioning

confidence: 99%

Differences in the attachment of Mycoplasma pneumoniae cells and membranes to tracheal epithelium

Gabridge¹,

Barden-Stahl²,

Polisky³

et al. 1977

Infect Immun

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Hamster trachea organ cultures were exposed to isolated membranes of Mycoplasma pneumoniae, PI 1428. Attachment, monitored by the uptake of tritiated membranes, was relatively insensitive to neuraminidase pretreatment, unlike the attachment of viable cells. Membrane attachment was optimal when explants were incubated with 50 to 100 ug of membrane protein per 'ml in minimal essential medium broth while gently being rotated (1 rpm) in a roller apparatus for 90 to 120 min at 3700. Saturation of the receptor sites with viable cells failed to inhibit subsequent membrane attachment. Induction of squamous metaplasia by extended cultivation of tracheal explants in a vitamin A-free medium reduced the content of ciliated cells without significantly affecting total cell viability, but did not alter the attachment of M. pneumoniae membranes. Collectively, the data indicate that the mechanism of attachment ofM. pneumoniae membranes to respiratory epithelium is distinct from the receptor sitemediated attachment of M. pneumoniae cells.

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Effect of Lipid a and Fatty Acids on Transport Activities of Membrane Vesicles From Escherichia Coli

Cited by 5 publications

References 6 publications

Inhibition of Growth and Uptake Processes in Bacteria by Some Chemical Food Preservatives

Inhibition of Growth and Uptake Processes in Bacteria by Some Chemical Food Preservatives

Involvement of autolysin in cellular lysis of Bacillus subtilis induced by short- and medium-chain fatty acids

Differences in the attachment of Mycoplasma pneumoniae cells and membranes to tracheal epithelium

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