Effect of Lipid-Hydroperoxide-Induced Oxidative Stress on Vitamin E, Ascorbate and Glutathione in the Rabbit Retina

Abstract

Background: It is possible that oxidative stress causes several retinal diseases. However, the natural biogenic role of antioxidants in the retina is not clear. Purpose: This study investigates the change in concentration of vitamin E (VE), ascorbate and glutathione (GSH) in the retina following vitreous injection of 600 µg 18:2 linoleic acid hydroperoxide (LHP) in male New Zealand rabbits. Method: LHP was injected above the retinal surface. The animals were sacrificed and the eyes enucleated before LHP injection, 1, 3, 6, 12, 24 h and 4 and 7 days after LHP injection. Retinas were removed, VE and ascorbate measured by HPLC, and GSH determined by a fluorometric method. Results: The concentration of VE in the retina decreased from pretreatment levels of 154.6 ± 29.7 nmol/g wet weight (n = 7) and was lowest at 6 h (61.1 ± 18.1 nmol/g wet weight, n = 4, p < 0.05), then increased gradually, returning slowly to pre-LHP levels by 7 days. The concentration of ascorbate in control retinas decreased at 6 h from pretreatment levels of 7.33 ± 0.93 µmol/g wet weight (n = 7) to 2.74 ± 0.16 µmol/g wet weight (n = 4, p < 0.05) and returned to pretreatment levels rapidly by 24 h after injection. The concentration of GSH in retinas decreased from baseline levels of 109.53 ± 8.19 µg/g wet weight (n = 9), was lowest at 12 h (72.40 ± 11.17 µg/g wet weight, n = 5, p < 0.05) and returned to pretreatment levels by 7 days. Conclusion: The results suggest that intravitreous LHP injection is a contributor to oxidative stress in the rabbit retina by causing a reduction in antioxidant capacity.

Panton-Valentine Leucocidin of Staphylococcus aureus Induces Oxidative Stress and Neurotransmitter Imbalance in a Retinal Explant Model

“…12 The drop of these antioxidants could reveal the switch from their reduced form to their oxidized form. Kamegawa et al 26 similarly reported a fall of the reduced form of several antioxidants, such as glutathione, ascorbate, and vitamin E in rabbit retinas a few hours after a pro-oxidative molecule (lipid hydroperoxide) was injected intravitreally. The present study showed that the antioxidant decrease is an early event after PVL treatment, because glutathione, taurine, and ascorbate dropped strongly only two hours after incubation with the toxin.…”

Metabolomics Analysis of the Aqueous Humour Uncovers Potential Metabolite Biomarkers for Early Differential Detection of Macular Edema

“…The structure and function of the retina rely largely on fatty acid composition [27] . According to evidence from epidemiological studies and animal experiments, the fatty acid composition of the retina is in uenced by diet [28][29][30] . The ndings in this study seem to suggest that nutrient intake and metabolic organ function may both affect ocular health through metabolism, providing new ideas for intervention strategies.…”

Puerarin prevents cataract development and progression in diabetic rats through Nrf2/HO‑1 signaling

“…Additionally, the groups treated with puerarin exhibited significantly higher AOC compared with the untreated diabetic group. GSH is an important antioxidant agent in the retina and serves a role similar to vitamins A, E and C, and selenium in the defense against cataract formation (46). In the present study, the levels of GSH in puerarin-treated rats were higher compared with untreated diabetic rats.…”