Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Sláma, Petr; Kabourková, Eliška; Sládek, Z.; Zavadilova, Terezie; Kratochvilova, Lucie; Kharkevich, Kristina; Roychoudhury, Shubhadeep; Pavlík, Aleš; Roztočilová, Andrea; Uhrinčať, M.; Tančin, V.; Kimura, Kazuhiro; Konečný, Roman; Kiku, Yoshio; Watanabe, Atsushi; Kwak, Jong‐Young; Zouharová, Monika

doi:10.3390/ani10060990

Cited by 3 publications

(10 citation statements)

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“…The lymphocyte portion gradually increased following S. uberis instillation into the mammary glands compared to in the controls (instillation of PBS). A similar result was noted in a previous study using S. aureus , S. uberis , lipopolysaccharides, and muramyl dipeptide to induce an inflammatory response in bovine mammary glands [ 9 , 12 , 23 ].…”

Section: Discussionsupporting

confidence: 88%

“…Contrary to that, the peptidoglycan of Staphylococcus aureus is able to induce lymphocyte apoptosis during the initial stages of mastitis [ 11 ]. Similar results have been obtained in experiments involving the lipopolysaccharide of E. coli or muramyl dipeptide [ 12 ].…”

Section: Introductionsupporting

confidence: 87%

“…The mammary gland sinuses were washed out with PBS to obtain a cell suspension, that was similar to the one that was used in a previously reported upon procedure [ 8 , 9 ]. In short, the first sample was obtained by a lavage of one quarter after 24 h following instillation with PBS, and the remaining quarters were washed out at 48 h, 72 h, and 168 h following the use of PBS [ 9 , 12 ]. The percentage of lymphocytes obtained from the lavages was analyzed by flow cytometry in dot plots with forward scatter and side scatter [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies [ 9 , 11 , 12 ], we have only investigated whole lymphocyte populations. Each s lymphocyte subpopulation may have a different sensitivity level for the induction of apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Sláma

Zavadilova

Pavlík

et al. 2021

Animals

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In this study, we focused analyzing γδ T cells during bovine mammary gland inflammation induced by Streptococcus uberis. A mammary gland cell suspension was obtained using lavage 24, 48, 72, and 168 h after intramammary-induced infection. The proportion of lymphocytes increased during the entire week in which inflammation was present. The γδ T cells were also elevated during inflammation, reaching their peak at 72 h following induced inflammation. The percentage of apoptotic lymphocytes continually increased, with the highest proportion occurring 168 h after S. uberis infection. The results show that γδ T cells may be involved in the resolution of inflammation in bovine mammary glands, with the apoptosis of those cells potentially playing an important role.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Sláma

Zavadilova

Pavlík

et al. 2021

Animals

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“…The apoptosis of lymphocytes is a process regulating the development of lymphocytes, as well as the functional potential of lymphocytes in diseases [ 86 , 87 ]. The same phenomenon occurs in granulocytes [ 88 , 89 , 90 ].…”

Section: Process Of Eosinophil Apoptosismentioning

confidence: 99%

Apoptosis of Eosinophil Granulocytes

Zustakova

Kratochvilova

Sláma

2020

Biology

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In the past 10 years, the number of people in the Czech Republic with allergies has doubled to over three million. Allergic pollen catarrh, constitutional dermatitis and asthma are the allergic disorders most often diagnosed. Genuine food allergies today affect 6–8% of nursing infants, 3–5% of small children, and 2–4% of adults. These disorders are connected with eosinophil granulocytes and their apoptosis. Eosinophil granulocytes are postmitotic leukocytes containing a number of histotoxic substances that contribute to the initiation and continuation of allergic inflammatory reactions. Eosinophilia results from the disruption of the standard half-life of eosinophils by the expression of mechanisms that block the apoptosis of eosinophils, leading to the development of chronic inflammation. Glucocorticoids are used as a strong acting anti-inflammatory medicine in the treatment of hypereosinophilia. The removal of eosinophils by the mechanism of apoptosis is the effect of this process. This work sums up the contemporary knowledge concerning the apoptosis of eosinophils, its role in the aforementioned disorders, and the indications for the use of glucocorticoids in their related therapies.

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Protein arginine N-methyltransferase 4 (PRMT4) contributes to lymphopenia in experimental sepsis

Lai

et al. 2022

Thorax

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BackgroundOne hallmark of sepsis is the reduced number of lymphocytes, termed lymphopenia, that occurs from decreased lymphocyte proliferation or increased cell death contributing to immune suppression. Histone modification enzymes regulate immunity by their epigenetic and non-epigenetic functions; however, the role of these enzymes in lymphopenia remains elusive.MethodsWe used molecular biological approaches to investigate the high expression and function of a chromatin modulator protein arginine N-methyltransferase 4 (PRMT4)/coactivator-associated arginine methyltransferase 1 in human samples from septic patients and cellular and animal septic models.ResultsWe identified that PRMT4 is elevated systemically in septic patients and experimental sepsis. Gram-negative bacteria and their derived endotoxin lipopolysaccharide (LPS) increased PRMT4 in B and T lymphocytes and THP-1 monocytes. Single-cell RNA sequencing results indicate an increase of PRMT4 gene expression in activated T lymphocytes. Augmented PRMT4 is crucial for inducing lymphocyte apoptosis but not monocyte THP-1 cells. Ectopic expression of PRMT4 protein caused substantial lymphocyte death via caspase 3-mediated cell death signalling, and knockout of PRMT4 abolished LPS-mediated lymphocyte death. PRMT4 inhibition with a small molecule compound attenuated lymphocyte death in complementary models of sepsis.ConclusionsThese findings demonstrate a previously uncharacterised role of a key chromatin modulator in lymphocyte survival that may shed light on devising therapeutic modalities to lessen the severity of septic immunosuppression.

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Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Cited by 3 publications

References 50 publications

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Apoptosis of Eosinophil Granulocytes

Protein arginine N-methyltransferase 4 (PRMT4) contributes to lymphopenia in experimental sepsis

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